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EMAGE:21684

Cnot3 CCR4-NOT transcription complex, subunit 3 ( MGI:2385261)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21684 EMAGE:21684 EMAGE:21684 EMAGE:21684 EMAGE:21684
"Pseudo-wholemount" of euxassay_005118. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005118_01 euxassay_005118_02 euxassay_005118_03 euxassay_005118_04
EMAGE:21684 EMAGE:21684 EMAGE:21684 EMAGE:21684 EMAGE:21684
euxassay_005118_05 euxassay_005118_06 euxassay_005118_07 euxassay_005118_08 euxassay_005118_09
EMAGE:21684 EMAGE:21684 EMAGE:21684 EMAGE:21684 EMAGE:21684
euxassay_005118_10 euxassay_005118_11 euxassay_005118_12 euxassay_005118_13 euxassay_005118_14
EMAGE:21684 EMAGE:21684 EMAGE:21684 EMAGE:21684 EMAGE:21684
euxassay_005118_15 euxassay_005118_16 euxassay_005118_17 euxassay_005118_18 euxassay_005118_19
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euxassay_005118_20 euxassay_005118_21 euxassay_005118_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21684Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21684_wholemount_strong.wlz
21684_wholemount_moderate.wlz
21684_wholemount_weak.wlz
21684_wholemount_possible.wlz
21684_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21684_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6339
Entity Detected:Cnot3, CCR4-NOT transcription complex, subunit 3 ( MGI:2385261)
Sequence:sense strand is shown

>T6339
ATACTCAAGCTATGCATCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAA
TTCGCCCTTCAACCAGTCTAGCAGTACACCCACCTCAACAACCTCCAGCTCTCCCATCCCACCCAGCCCA
GCAAACTGCACTACGGAAAACTCTGAAGATGATAAGAAGAGAGGCCGATCTACAGATAGTGAAGTCAGCC
AGTCTCCAGCCAAAAATGGCTCCAAGCCTGTCCACAGCAACCAGCACCCCCAGTCCCCAGCTGTGCCGCC
CACCTACCCCTCTGGCCCCCCACCTACCACTTCTGCCTTGAGCTCCACCCCTGGCAACAATGGGGCCTCT
ACCCCAGCAGCACCTACAAGTGCCCTGGGCCCTAAGGCCAGTCCAGCTCCCAGCCACAACTCGGGTACTC
CTGCACCCTATGCCCAGGCTGTGGCCCCACCTAATGCCAGCGGGCCCAGCAATGCCCAGCCACGGCCCCC
CAGTGCCCAGCCAAGCGGGGGAAGTGGTGGTGGCAGCGGAGGGAGCAGCAGCAATAGTAACAGTGGCACA
GGCGGAGGGGCTGGCAAGCAGAACGGTGCCACAAGCTATAGTTCGGTTGTGGCAGACAGCCCTGCA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAACCAGTCTAGCAGTACACC; Reverse Primer - name:unspecified, sequence:ACAATGGTATGTTCACCTCTG. The reverse primer contains a 5' extension containing an T7 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using T7 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_005118 same experiment
 EMAGE:21685 same embryo
 EMAGE:21683 same embryo
 EMAGE:21682 same embryo
 EMAGE:21681 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS