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EMAGE:21745

Slc27a6 solute carrier family 27 (fatty acid transporter), member 6 ( MGI:3036230)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21745 EMAGE:21745 EMAGE:21745 EMAGE:21745 EMAGE:21745
"Pseudo-wholemount" of euxassay_018867. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018867_01 euxassay_018867_02 euxassay_018867_03 euxassay_018867_04
EMAGE:21745 EMAGE:21745 EMAGE:21745 EMAGE:21745 EMAGE:21745
euxassay_018867_05 euxassay_018867_06 euxassay_018867_07 euxassay_018867_08 euxassay_018867_09
EMAGE:21745 EMAGE:21745 EMAGE:21745 EMAGE:21745 EMAGE:21745
euxassay_018867_10 euxassay_018867_11 euxassay_018867_12 euxassay_018867_13 euxassay_018867_14
EMAGE:21745 EMAGE:21745 EMAGE:21745 EMAGE:21745 EMAGE:21745
euxassay_018867_15 euxassay_018867_16 euxassay_018867_17 euxassay_018867_18 euxassay_018867_19
EMAGE:21745 EMAGE:21745 EMAGE:21745 EMAGE:21745 EMAGE:21745
euxassay_018867_20 euxassay_018867_21 euxassay_018867_22 euxassay_018867_23 euxassay_018867_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21745Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21745_wholemount_strong.wlz
21745_wholemount_moderate.wlz
21745_wholemount_weak.wlz
21745_wholemount_possible.wlz
21745_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21745_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T51037
Entity Detected:Slc27a6, solute carrier family 27 (fatty acid transporter), member 6 ( MGI:3036230)
Sequence:sense strand is shown

>T51037
CCTGGTCTCCTCATTTCTCGTGTGAATAAGAAGAATCCCTTCTTCGGCTACGCTGGCTCGGACACGCACA
CGAAAAGCAAGCTGCTCTTTGATGTGTTTCGGAAGGGAGACGTGTACTTCAACACCGGAGACCTAATGTT
CCAAGACCAGGAGAACTTCGTTTATTTTTGGGACCGTCTTGGAGACACTTTCAGGTGGAAAGGAGAAAAC
GTCGCAACCACAGAGGTCGCTGATGTGCTTGGGAGGTTGGACTTCATCCAGGAAGCAAACGTGTACGGTG
TGCGTGTGCCAGGTTATGAAGGAAAAGCAGGAATGACTTCTGTTATCCTAAAACCAAACAAGTCTTTAGA
CTTAGAAAAAATGTACAACCAAGTGGTGACATCTCTGCCAGCTTACGCCTGCCCACTGTTTTTAAGAATT
CAGGACAAAATGGAAACTACAGGGACATTCAAGCTAAAGAAGCTACAACTGGTGGAAGAAGGATTTGATC
CGCTGAAGATTTCTGACCCGCTTTACTTTATGGATAACTTGAAAAAATCTTATGTCCCATTGACCGAAGA
GATTTATAATCAGATAATGTCAGAGGAAGTTAAACTGTAAAGACTGTCTTTGTGGGACTTTGATGACTTC
CTGGGAAAGATGAAGGAAGATTCGTGAGCTTCCTTGTGGACAATCAGGGAGGAGTTCCTTGCATTAATCA
ATCATAAATAGAAAGAAAATTGTACATGAAGAATGCTTTGTAGACCCAAGCTTTCAAGGCCCCCACTCTG
CAGCACAGTGGGCCATGAAGTGGGAGCTGTTTGCCTCGTGGTTATGTTATGAGGCTGGCTTGAGGATGCC
GCTTACAGACGCTGCCCGGAGTCACCAGGAAGCAGCATACTTCATAGCATGAGCCCAGAGTACAGCGATT
TCTGAATGCGTTTGGGAAGTAAAAGCCA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CCTGGTCTCCTCATTTCTCG; Reverse Primer - name:unspecified, sequence:TGGCTTTTACTTCCCAAACG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_018867 same experiment
 EMAGE:21746 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS