Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29274

2210404O07Rik ( MGI:1919523)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29274 EMAGE:29274 EMAGE:29274 EMAGE:29274 EMAGE:29274
euxassay_006454_01 euxassay_006454_02 euxassay_006454_03 euxassay_006454_04 euxassay_006454_05
EMAGE:29274 EMAGE:29274 EMAGE:29274 EMAGE:29274 EMAGE:29274
euxassay_006454_06 euxassay_006454_07 euxassay_006454_08 euxassay_006454_09 euxassay_006454_10
EMAGE:29274 EMAGE:29274 EMAGE:29274 EMAGE:29274 EMAGE:29274
euxassay_006454_11 euxassay_006454_12 euxassay_006454_13 euxassay_006454_14 euxassay_006454_15
EMAGE:29274 EMAGE:29274 EMAGE:29274 EMAGE:29274 EMAGE:29274
euxassay_006454_16 euxassay_006454_17 euxassay_006454_18 euxassay_006454_19 euxassay_006454_20
EMAGE:29274 EMAGE:29274
euxassay_006454_21 euxassay_006454_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35023
Entity Detected:2210404O07Rik, ( MGI:1919523)
Sequence:sense strand is shown

>T35023
CTGGCTACACTGTTCCTGACACCCGCTGAGGCCCAGCAGGCCTCAGAGCGCCGCCTGAAGCCTTGGCTAG
TAGGCCTGGCTGCAGTAGTGGGCTTCCTGTTTATAGTCTTCATCCTCATGCTGGCCAATCGTGTCTGGTG
CGCCAAAGGCAGAGCAGAGGATGAGGAGGCTACATTCAGAATGGAGCACATTATGAACGAGAACTCACAG
CCAAGTAAAGCAGACAAGAAGCAGAAGAAGAAGGTGGACAGGAAGGGCGGACAGAGCAACGAGGCCTTGG
AACTAGAGGAAAAGGAGTCCTCAGATGAGGAGAGAGGCAAGAAAACAGCTTTGTAAAGCATCTTGTCACC
TGGGGGTGCTTCATCCACACACCTCAAGTTGCAGCCACCACCTGAAGCCGGACTGGTGTCATCTGGGTCT
GAAAACTGTCCTCATATGAGGAGGTCAGCTCTGCCTATGGCCTCCATACTCACCCCCACCTTCCCCTGAC
ACTCACCACTGGCCTGGTGTCCACTCCTATTCATCTGTCCCCCTTCTGCATCCTCTCTCACATCCAATCT
GCCCCCTCCAGGCATGCTCCTGGTGTCACCTGACTGTCCCCTATGTCTCTGAGCCCCAGTGCTGTACCCG
GGAGGCTTCTACCCTCTGTTTTGGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 82766. Forward Primer - name:082766_F_cDNA_2210404O07Rik, sequence:CTGGCTACACTGTTCCTGACAC; Reverse Primer - name:082766_N_SP6_cDNA_2210404O07Rik, sequence:TCCAAAACAGAGGGTAGAAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006454 same experiment
 EMAGE:29491 same embryo
 EMAGE:29243 same embryo
 EMAGE:29242 same embryo
 EMAGE:30894 same embryo
 EMAGE:31368 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS