EMAGE mouse gene expression spatial database

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EMAGE:29379

Prdm5 PR domain containing 5 ( MGI:1918029)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_000286_11	euxassay_000286_12	euxassay_000286_13	euxassay_000286_14	euxassay_000286_15

euxassay_000286_16	euxassay_000286_17	euxassay_000286_18	euxassay_000286_19	euxassay_000286_20

euxassay_000286_21	euxassay_000286_22	euxassay_000286_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3677
Entity Detected:	Prdm5, PR domain containing 5 ( MGI:1918029)
Sequence:	sense strand is shown >T3677 TGGCCTCGAGCCAGATTCGGCACGAGGGGNCAAAAGGAGAAAGTCTGCCCATATTGTGGCCAGAAATTTG CCAGCAGTGGGACCCTGAGAGTTCACATCCGGAGCCACACAGGTGAGCGCCCCTATCAATGCCCGTACTG TGAAAAAGGTTTCAGTAAAAATGACGGACTGAAGATGCACATTCGTACTCACACCAGGGAGAAGCCCTAC CAGTGCTCAGAGTGCAGCAAGGCCTTCAGCCAGAAGCGGGGCCTCGATGAACACAAGAGGACACACACAG GAGAAAAGCCTTTTCAGTGTGACGTATGTGACTTGGCTTTTAGCCTGAAGAAAATGCTTATTCGACACAA GATGACACACAATCCTAACCGTCCGATGGCAGAGTGCCATTTCTGCCATAAGAAGTTTACAAGAAATGAC TACCTCAAAGTGCACATGGACAACATCCATGGGGTAGCTGACAGCTAAGAGGAGCGGCAAGGAACCACAC CATGTGAAAGAGCTTCTACTATGAATCCCAGATTCTTCTCACCTGATCGGCTTAACAGAAATAGC
Notes:	The probe template was PCR amplified from IMAGE:352184 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:352184 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000286 same experiment
	EMAGE:31181 same embryo
	EMAGE:29381 same embryo
	EMAGE:29850 same embryo
	EMAGE:29411 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source