EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29421

Gp9 ( MGI:1860137)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000271_01	euxassay_000271_02	euxassay_000271_03	euxassay_000271_04	euxassay_000271_05

euxassay_000271_06	euxassay_000271_07	euxassay_000271_08	euxassay_000271_09	euxassay_000271_10

euxassay_000271_11	euxassay_000271_12	euxassay_000271_13	euxassay_000271_14	euxassay_000271_15

euxassay_000271_16	euxassay_000271_17	euxassay_000271_18	euxassay_000271_19	euxassay_000271_20

euxassay_000271_21	euxassay_000271_22	euxassay_000271_23	euxassay_000271_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3724
Entity Detected:	Gp9, ( MGI:1860137)
Sequence:	sense strand is shown >T3724 TGGCCTCGAGGCNAGATTCGGACGAGGGCAGCCTTGAACAGGATGGTACCAGCCCACAAAAGGTGTGCCC CAGCCAAGGCCTCACAGCCTGCCTCCTGGATGACTACCTGGGGCCTCCTGTTTCTGCTCTGGCCTGCGAC CACAGATACTCAGGCTTGCCCCAGGCCCTGTACCTGCCAGTCCTTGGAAACCATGGGGTTAAAGGTGAAC TGTGAGGGGCAAGGCCTCACAGCCCTGCCCGTGATACCAGCGCACACTCGCCAGCTCCTGCTGGCCAACA ATAGCCTGCGTTCAGTGCCACCAGGTGCCTTCGACCACTTGCCTCAGCTGTGGGACCTGGATGTGACACA CAACCCCTGGCACTGTGACTGCAGCCTCACCTACCTGCGCCTCTGGCTGGAGGACCACATGCCCGAGGCC CTAATGCATGTGTACTGTGCCAGCCCTGACTTGGCCACCAGGCGCCCGCTGGGCCAGCTGACAGGTTATG AGCTGGGCAGCTGTGGCTGGAAGCTGCCACCATCCTGGGCCTATCCAGGGGTGT
Notes:	The probe template was PCR amplified from IMAGE:355508 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:355508 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000271 same experiment
	EMAGE:30977 same embryo
	EMAGE:29293 same embryo
	EMAGE:29296 same embryo
	EMAGE:31173 same embryo
	EMAGE:29851 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source