EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29622

Trpc5 ( MGI:109524)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007415_01	euxassay_007415_02	euxassay_007415_03	euxassay_007415_04	euxassay_007415_05

euxassay_007415_06	euxassay_007415_07	euxassay_007415_08	euxassay_007415_09	euxassay_007415_10

euxassay_007415_11	euxassay_007415_12	euxassay_007415_13	euxassay_007415_14	euxassay_007415_15

euxassay_007415_16	euxassay_007415_17	euxassay_007415_18	euxassay_007415_19	euxassay_007415_20

euxassay_007415_21	euxassay_007415_22	euxassay_007415_23	euxassay_007415_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35305
Entity Detected:	Trpc5, ( MGI:109524)
Sequence:	sense strand is shown >T35305 TGGTTCAACAACACCTTCTGTCCCAAAAGAGACCCTGATGGAAGACGAAGGAGGCACAACTTGAGAAGCT TCACAGAACGTCATGCTGACAGCCTGATACAAAATCAACATTATCAGGAAGTTATCAGAAATTTAGTCAA GAGATATGTGGCTGCAATGATAAGAAACTCCAAAACAAATGAGGGGCTAACAGAAGAAAATTTTAAGGAA TTAAAGCAGGACATTTCCAGCTTTCGATATGAAGTGCTTGACCTCTTGGGAAACAGGAAACATCCAAGAA GAAGCCTCTCCACCAGCAGTGCTGATTTCTCTCAAAGGGATGATACCAATGACGGCAGTGGTGGGGCTCG GGCCAAGTCTAAGAGTGTCTCTTTCAATGTAGGCTGCAAGAAAAAGGCCTGTCACGGTGCACCGCTCATC AGAACCGTGCCAAGAGCTAGTGGCGCCCAAGGAAAGCCAAAATCCGAGTCGTCGAGCAAACGGTCCTTCA TGGGTCCGTCTTTCAAGAAATTGGGTCTCTTCTTCTCCAAGTTCAATGGTCAAACATCTGAACCTACTTC GGAGCCAATGTACACAATCTCTGATGGCATCGCACAGCAGCATTGTATGTGGCAGGACATCCGCTATTCT CAGATGGAGAAAGGGAAGGCAGAGGCCTGTTCTCAAAGTCAGATGAACCTTGGCGAGGTAGAGCTAGGTG AAATCCGGGGGGCTGCTGCTCGGAGCAGTGAGTGCCCCCTAGCCTGTTCCAGCTCCCTTCACTGTGCATC CGGCATCTGCTCCTCAAATTCGAAACTTTTGGACTCGTCAGAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89676. Forward Primer - name:089676_F_cDNA_Trpc5, sequence:TGGTTCAACAACACCTTCTGTC; Reverse Primer - name:089676_N_SP6_cDNA_Trpc5, sequence:CCTCTGACGAGTCCAAAAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007415 same experiment
	EMAGE:29342 same embryo
	EMAGE:30051 same embryo
	EMAGE:30047 same embryo
	EMAGE:30940 same embryo
	EMAGE:30048 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source