EMAGE mouse gene expression spatial database

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EMAGE:29714

Il17e ( MGI:2155888)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000411_18	euxassay_000411_01	euxassay_000411_02	euxassay_000411_03	euxassay_000411_04

euxassay_000411_05	euxassay_000411_06	euxassay_000411_07	euxassay_000411_08	euxassay_000411_09

euxassay_000411_10	euxassay_000411_11	euxassay_000411_12	euxassay_000411_13	euxassay_000411_14

euxassay_000411_15	euxassay_000411_16	euxassay_000411_17	euxassay_000411_19	euxassay_000411_20

euxassay_000411_21	euxassay_000411_22	euxassay_000411_23	euxassay_000411_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3826
Entity Detected:	Il17e, ( MGI:2155888)
Sequence:	sense strand is shown >T3826 TGGCCTCGAGCCAGATTCGGACGAGGCGTCACGAGGCCCGGAGGAGTGGCTGAAGTGGAGCTCTGCATCT GTGTCCCCCCCAGAGCCTCTGAGCCACACCCACCACGCAGAATCCTGCAGGGCCAGCAAGGATGGCCCCC TCAACAGCAGGGCCATCTCTCCTTGGAGCTATGAGTTGGACAGGGACTTGAATCGGGTCCCCCAGGACCT GTACCACGCTCGATGCCTGTGCCCACACTGCGTCAGCCTACAGACAGGCTCCCACATGGACCCGCTGGGC AACTCCGTCCCACTTTACCACAACCAGACGGTCTTCTACCGGCGGCCATGCCATGGCGAGGAAGGTACCC ATCGCCGCTACTGCTTGGAGCGCAGGCTCTACCGAGTCTCCTTGGCTTGTGTGTGTGTGCGGCCCCGGGT CATGGCTTAGTCATGCTCACCACCTGCCTGAGGCTGATGCCCGGTTGGGAGAGAGGGCCAGGTGTACAAT CACCTTGCCAATGCGGGCCGGGTTCAAGCCCTCCAAAGCCCTACCTGAAGCAGCAGGCTCCCGGGACAAG A
Notes:	The probe template was PCR amplified from IMAGE:419419 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:419419 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000411 same experiment
	EMAGE:29716 same embryo
	EMAGE:29809 same embryo
	EMAGE:29808 same embryo
	EMAGE:29709 same embryo
	EMAGE:29663 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source