EMAGE mouse gene expression spatial database

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EMAGE:29728

4921509E05Rik ( MGI:2441675)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007043_01	euxassay_007043_02	euxassay_007043_03	euxassay_007043_04	euxassay_007043_05

euxassay_007043_06	euxassay_007043_07	euxassay_007043_08	euxassay_007043_09	euxassay_007043_10

euxassay_007043_11	euxassay_007043_12	euxassay_007043_13	euxassay_007043_14	euxassay_007043_15

euxassay_007043_16	euxassay_007043_17	euxassay_007043_18	euxassay_007043_19	euxassay_007043_20

euxassay_007043_21	euxassay_007043_22	euxassay_007043_23	euxassay_007043_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9438
Entity Detected:	4921509E05Rik, ( MGI:2441675)
Sequence:	sense strand is shown >T9438 AAACTTTTAAGCAGCAAATAGACACCCTTAGCTGCTGGGCGCTGGCGTATATAATTTTAGCGTAGATCTG GGCAGGTTAGAGCCCTGGCGAAGGCACTACAGGTACATCTATTACGTTCGTCCGGTCTTTGTTGGGTATC AGATGTTCATCTAGAAATGCATTTCTGAAACACAGACTTCAGATAGCTGAGCTGTTCTGCAGCTTCTAAG TTCAACATGTATGAAGTTTTGCTGGGTTCTGCTCCTGAGAATGAAGGCCGCCTTGTGACAGTGAAGGTGG AAGATCCCACCTGGGGACAGCCAGGCAACTCACAGGAGGGCAGGGCGCATGCTCAGGAACTTTACCGCCT GCGATTTCGGCACTTCTGTTACCATGAGGCAGACATATCCTCTGCAGAGAGGTGATGAAGCGGCGACATT ACTGGAGAATCTAGCCTCGGAACGTGGAGACACAGGGCAGCAGGCTACTCTCTGTGCTCAGGAACAGGAC ATGCACCTAGTGGTGACAGAGTATCCAGGAGCCTCTATGGAGGAGTATCAGAGCCTCCATCTCCTGCAGT CTTCGGTCACTCCCTGGACAAGAGACTTTCCAGAGCTCCCCCTGGGATGTGAGAGAGATTCTCCTTCCCC CACTGAAAAGGCAGTCTCCACTTTGAACACTGTCGGGAGCTGCCACCCACCCCATGACTCCTGGGCCAAG ATGCACATTGCGTCCTTGCAGTACATTACTCAGAAAGGGAGGGAAAAAGCCAAATTTAGAGTGATCGAGC TGCTCCAAGGCCATGCGTTTTCTGATGAGTCAGAGACAGAGGAAGACAATGAGCCTGAGGTACAGCCTGA GAGAAAGAAGATAAAGGCATCCAGCATCCCTGAGAAGAGCTGGACCAAACGAGACAGTAAGCCTAACTTT CCAAGCTGGTCAGCCCTGGATTCTGGACTTTTGAATCTTAAGAGTGAGAAGTTGAACCCAGTAGAGCTCT TTGAATTATTTTTTGATGGTGAAACATTCAACTTGATTGTCAATGAAACCAATAATTATGCTTCTCAGAA AAATGTCAGCCTAGAAGTCACAGTGCAGGAAATGAGGTGTGTGTTTGGTGTTCTGCTCTGCAGTGGATTT GTAAGGCATCCCAGAATGGGGATGTATTGGGAAATCTCTGATTCTGATCAGACCCTGGTTAGAAATGAAA TTAGAAGGGACAGATTTGAATTAATTTTCTCATGCCTGCATTTTGCAGATAATAAGCACCTCGATCAAAA AGATAAGTTTTCAAATTTGAGGCCTCTCATAAAGCAAATGAAAAATTTTCCTCTTGTATGCCCCCCTGAG GAATACTATTGTTTTGACAAGTCAATGTGTGAATGCTTTGATTGTGACCAATTTCTGAATGGGAAGCCTC TTCAAATTGGCTATAAAATTTGGTGTGGTACAACTAACTACGCAAGGCTATCTGGTTTGGTTTGAGCCTT ACCAAGAAGACTCGGGTGTGGATAAGGAGCTGGACCTTGGGCCAGGTGGAAATCTAATAATGAGTTTTGC TGATGTTCTTCTGGAGAAAGGTCACCATCCCTATCACTTGAGTTTTGATAGCTTCTTTACACCTGTTAAA CTGATGTCAGCTTTGAAGAAGAAGGGAGTGAAGGCTACAGGATCGCTTTGTGAAAACAAAACAAAAATGT CCACTTATGAATGTGGAACTTATGAAAAAGATGAACCGGGGGTATTTTGATTTCATAGTGGAAGAAAAGG AGGAGATATTTTTATGCCGTTGGCATGGTGATGGCTTTATTAGTCTGTGTTCAAATGCTGTAGGCATAGA ACCAGTCAGCAAAGCAAGCTGTGTAGCTGACGATAAGGAGTTCCTTCCTTCAGGTAACATCCATTGTGAA GTTATGTGAGGAATGCAGGAAAGGTGTAGCTAAAATGGATCAAATTATTTCCAAATACAGGGTGGGATTA AGAAGTAAGAAGCGGCTCTGGGTTTTGGTGAGTTACATGGTCGATGTAGCCATGAGCAATGCGTGGCAGT TGCACAGAATCTGTAACCCAGGCTCCCCTTTAGATCTCCCAGGTTTCCAGAAATGTGTGGCATGTTTTCG CTTGGGACATGATGCTAATCTGTCTGATTAGGACGCGTAAAGAACACAGTAACAACACACACACACAAAT TATAAGTGTAGTAAATCTACATTACCAAAGCAAATGTAATGGAGAAGGTGTGATTATGTCACTGGTATTG TTTACATCAAGGATTGTTACAGCGAACAATGCTTTAAAAATAACATTCTGTGAGTGTTGGATTTGTGTTG CTTTTATGTCTAGTTTTGGGTCAGATATATGAAATGACTTACTTGTTAAATATTTTATATATTAAAAGAG CAAAAACCTACCATTTCCTCACTTTCAGTTTTGTTTTAGAGATTGACATTTTGAAAATGCAGGCAATATC ACACTCTATAGAATGACACTGAATTCATAGTCCAGAATAGATACAAAACAGATATACAACTCATGCGGGA GGATATGCTAACGGATATAGGTATAAGGGAGTAAATATTTCAGCTGGATCACACTATATGTATATAGTTG AGACAGGGCCAGGCATGAAAATTGCTCTGTGTGTGTGTGAGTGTGTGTGTGTGTGTGTGTGTAGCTGTGC CTTTGAATACTGAAGAAAATGTTATGAGTTGGGAGAAGTCAGAGAGAGGAAAGTAATGAAGCAATCTGTG TAACAGTTATCCTGAGAAGAAAGCAAGTATAAGGTGATTGGACATAGTCTAGATTGAGGTCATCAGTGGT TACTCATCCAATGAGAGACATTTGGAGCAAGTGAACTATGACTCCATAATGCTGAAGATTTGTAGGGGTA AATTAAACTAAAGGCAAGGAGGATTCGACACCATTTCTGTGATTATTATTTAATTATTTATATAAAATAA ACAAACACATCTCATTAGTTCTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30613267 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30613267 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007043 same experiment
	EMAGE:31121 same embryo
	EMAGE:30859 same embryo
	EMAGE:31730 same embryo
	EMAGE:31821 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source