EMAGE mouse gene expression spatial database

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EMAGE:30009

Nadk ( MGI:2183149)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005377_01	euxassay_005377_02	euxassay_005377_03	euxassay_005377_04	euxassay_005377_05

euxassay_005377_06	euxassay_005377_07	euxassay_005377_08	euxassay_005377_09	euxassay_005377_10

euxassay_005377_11	euxassay_005377_12	euxassay_005377_13	euxassay_005377_14	euxassay_005377_15

euxassay_005377_16	euxassay_005377_17	euxassay_005377_18	euxassay_005377_19	euxassay_005377_20

euxassay_005377_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6102
Entity Detected:	Nadk, ( MGI:2183149)
Sequence:	sense strand is shown >T6102 ACAGCACACAGGTCTCAGGAGTTCCCACCAGAGAACATCCTGACTGCAGCAGGCACCAGAGTTTTTCTCA TATCAACTATGGAAATGGAACAAGAAAAGATGAACCTGAGTCAGGAGCTGAGTGCCGACTCAGCCTCCTA CAACTGCTCAGCATGTCATGGGGATGAGACCTGGAGCTACAATCACCCTATCCGGGGCCGGGCCAAGTCT CGGAGCCTGTCAGCCTCACCTGCCCTAGGCAGCACCAAGGAGTTCAGGAGGACCCGCTCTCTCCATGGAC CGTGCCCAGTGACAACATTTGGACCAAAGGCCTGCGTGCTGCAGAACCCGCAGACCATCATGCACATCCA GGACCCTGCAAGTCAACGGTTGACATGGAACAAGTCTCCCAAGAGTGTGCTTGTCATCAAAAAGATCCGA GATGCCAGCTTACTGCAGCCCTTCAAAGAGCTCTGCATATACCTCATGGAGGAAAACAACATGATCGTGT ATGTGGAAAAGAAAGTTCTGGAAGACCCTGCTATAGTGAGTGATGAAAACTTTGGACCAGTGAAGAAGAA GTTCTGCACTTTCCGTGAAGACTATGATGACATTTCCAACCAGATAGACTTCATCATATGCCTTGGAGGA GATGGCACCTTGCTCTATGCCTCCTCACTTTTCCAGGGCAGTGTGCCTCCTGTTATGGCCTTCCACCTTG GATCCCTGGGCTTTCTGACCCCCTTCAACTTTGAGAACTTCCAATCCCAAGTGAATCAGGTGATAGAGGG GAATGCCGCTGTCATCCTGAGGAGCCGGCTGAAGGTCAGGGTGGTGAAAGAGCCCAGAGACAAGAAGACA GCCATCCACAACGGCCTCAGTGAAAATGGTCTGGACACAGAGGGCGGAAAACAGGCTATGCAATACCAGG TCTTAAATGAAGTGGTGATCGACAGAGGCCCCTCCTCATATCTGTCCAACGTGGATGTCTACCTGGATGG ACACCTTATCACTACTGTGCAGGGCGATGGAGTGATCGTGTCCACCCCGACGGGCAGCACAGCATATGCA GCTGCAGCCGGGGCCTCCATGGTCCACCCCAACGTGCCGGCCATCATGGTTACACCCATCTGCCCCCACT CGCTGTCATTCCGGCCTATCGTGGTCCCTGCAGGGGTCGAGCTGAAGATTATGCTGTCACCGGAAGCCAG GAACACGGCATGGGTGTCTTTTGATGGACGGAAGAGACAGGAAATCCGCCATGGAGACAGCATCAGCATT ACCACCTCCTGCTACCCGCTGCCCTCCATCTGTGTGTGCGACCCTGTGAGTGACTGGTTCGAGAGCCTTG CCCAGTGTCTGCACTGGAATGTTCGAAAGAAGCAAGTTCACTTTCCAGAGGATGAGGAGGACAGCTAGAC TACATTTCCCAGCTTCCAACCTGAGCAGACCCTCAAGCTCTTGGGGGCCTTCCTTCTCTTGCTCTCCTGC TGGCCTTCTGGCTGACCTAGCCTTGACAAACCACATGGCTGTCCAGGCATCTATGTCCTGCCTGCCTTGC TGGGAGAGCTTGGGTCTGCCTTTTGCAGAACAGATCAGCTTTTTAATGTTTTTAAAACCTCTTTTTCATT TCTAAAAAAGCAAAATGAGAACTGGGCTGGGTGTCCTACATCTCTCCCATGGGCCCCCGTATCCCCCTGA GATTCCCAGGTCAGGAATTCCCATAAATCAGTCTGTTGAAATTCTGAGGGGGTCAGAATGAATTGATCTT CCTTTTGATACTGAAATAAATGTCTCAGCCAGAAATCTTCTTTTAACTGCAGTGCTTTTCCACAGGGCCT GTACCTTTCCCCCTGGCAGCTTTGCTATGGGTGGGCAAGGCAAGCTTGCTTTTTGTGTTTCTGGAGATGG CTAGGTCCCTCTCAGTCTGGCCCCCAGACCTCTGACTCCTTCCAGAGCAGACAGAGGCAACACTTGGCAC AGCCATCCAACAACCTCACATTCCTCACACAACAGGGCAGCAGCTCCTGGGGACAGTTTAAATCCTGTAT ACACTTTTTAGAGGTTATTTTAAACTTTCTGAAGTGATTATGTACATATTTTCTTGTACACACTTTGTGA AGATTTAGAGGGGACTGCCTGTTGTCCACTGTTTACATTGTGTCCAGGACAGGGCTCTGTGCTTCCACTC CTCCCTTACACAGTGGCCACAAGGGTTTTGAGACCAGCTCACTATTGACATCAGGCTCGTGGGCCCGCTC TCCTTCCATAAGAATCTTAGGTTTGATTCCATCTGAACAGCAGCCATGTGGAGCCTGTGTGCGAGATGCA TGGAGCCATGTAATGCAAGTAACATTAGCACGTGAGTGCCACACAGGCCGTGAGTGCCACACACGCCCTA GAGCCTTTGTGGAGTCAGTGGTCAGCCACGTTGTCTCAGCTCGCTAGCTACTTTCAGTTTTGTCCTCACC TCTTGCAGCAGGAGTCGGAAGGAAGGACTGCCTGTCCTTTGACTGGCTTTTGTCTCCTGCTTCTAGGAAA GGTTTGTCAGTCGGCTGCTGTAGAACCGACCCCACTGAGCTTTCCTACCTGTTAAAGTCAGGACCTACTG AGCACCCATGGAGCCCTAAGTGGTCTGGGTTCAGTGCTGTTATCATGGTGACTCGTCCCCACGGTCTGTG CTGTTCATCTGTGACTGAGCACAGCAGGCCTCTGCTATGATCCAGAGGAGAGCAGGGTCTGAAGTGTGTG CCACCCATCTCAAAAGCAAGTGTTGACAAATCTAGAAGCAGCTGCCACCAGACTGATCTAGAACACATGG AGTTTTCACGTAAATTCTTAATGTTTCATATTGTTAATATCTTAAAATTTTGTTAATTGTTAATGAAGGC CTTATTACTATTTTCAGATTCTTTCAATAAATAAACTTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:3493623 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3493623 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005377 same experiment
	EMAGE:30020 same embryo
	EMAGE:30036 same embryo
	EMAGE:30795 same embryo
	EMAGE:30881 same embryo
	EMAGE:30033 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source