EMAGE mouse gene expression spatial database

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EMAGE:30128

2610024E20Rik ( MGI:2447834)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004910_01	euxassay_004910_02	euxassay_004910_03	euxassay_004910_04	euxassay_004910_05

euxassay_004910_06	euxassay_004910_07	euxassay_004910_08	euxassay_004910_09	euxassay_004910_10

euxassay_004910_11	euxassay_004910_12	euxassay_004910_13	euxassay_004910_14	euxassay_004910_15

euxassay_004910_16	euxassay_004910_17	euxassay_004910_18	euxassay_004910_19	euxassay_004910_20

euxassay_004910_21	euxassay_004910_22	euxassay_004910_23	euxassay_004910_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6924
Entity Detected:	2610024E20Rik, ( MGI:2447834)
Sequence:	sense strand is shown >T6924 CCCGCTCGCCGTTAGGGAGGCTCTGCGCCTCAGCCGCCACCGCCTCCGCCTCCGCCTCCGCCGCTCCCGC CCACTTCTCCGCGTCTGCCGCCGCCGCTGCCCGGGGTCTCCGGGGGGTCGCTACCGGGCTGGCCCCGCGC CACTGCAGTCGCTTCAGCCTCGCGCTAGAGGCCGCTTCAGGAGGAGACCCGCCGGCCGCCGTGCCACTTA GGGCCTCAGTTCTCGCCTCGTCGACGTCGCCAAGGGTTCTGGAAAAGCGGCCAAGGCCGGGGAACGGGGC TTCGCGGTTCCAACGATTAAGTTCTGAAGTACTGATCGAGTTCTGCGTTTCTTCAATGAGGAACTACAGG CTGATCTTCTGCCATAATCTCAAACAGCCATAAATGACAGAACGATGCTTGCTCAGTGAGGGAAGCTGGT GCAGAAGCCGTGTTTTAAACTTAGGTGTTTAAGTACTCAAAGAAAAGACAGCTTTGATTTCTGGCTGCAA AAAAGCTATGAGGAAGAGCTCCTCCCCTTCCTTGAGTAACTGCAACTCCGATCTTGCTAGCAAAATATTT GGAATTCCACTTGATGAGCTGCAGCAGGGAGGACATCCAGACAATGAGGTTCCGTTCATAGTCCGCCACG TTGTGGACTATATTGAGGAACATGGAGGTCTGGAGCAACAAGGGCTGTTTCAAGTCAATGGGAATGCTGA GACAGTGGAGTGGCTTCGGCAGAGATACGACAGTGGAGAAGAGGTGGACTTGGTTAAGGAAGCGGACGTC CCCTCAGCCATTAGTCTCCTTAGGTTTTTCCTGCAGGAGCTCCCTGAGCCCGTCATACCTGGCAGCTTGC ATATTCACTTGCTGCAGCTTTCTCAAGATTATAATAATGAAGATGAATTTGGAAGAAAGTTGAGGTTCCT CTTGCAACAGCTTCCACCTGTTAATTACAGTTTGTTAAAGTTTCTGTGTAGGTTTTTAGCCAATGTAGCA TCACATCATGAAGAAATTTGGTCTGCAAATTCTTTGGCTGCTGTTTTTGGTCCAGATGTCTTCCACATTT ACACGGATGTGGAAGATATGAAAGAGCAGGAAATTGTGAGCAGGATAATGGCGGGACTTCTAGAAAATTA TTACGAGTTTTTTGAGAATGAAGAGGAAGATTTTTCATCAAATGATTTGAGTTCAATTACTGAGCAGGTT AATGAACTTTCTGAGGAAGAAGAGGAAGATGAAAAGCTGGAGCATATAGAAGAACTTCCAGAAGAAGGTG TAGAAAAGTCAGCTGGCATGCCAGAGGTGCTGCAGTTAAGGATGACTGAAAACCTTCTGGACTCAGACAG TGTTACAGCATCAACAAGGATTGATGCTGCTGCTGCTACTACTACTAATGCCAGTGATGGTAACATTAAG TGTTCAAAACCTGTGGCTGGTACTACTGCAGATAATGAAGTCATGCAGCAAGATTTTGTATTTGAGGATC AGAAAAATAATGAGTCTGTAGGTATACTGTTAGAGCCTTGTAGTGACCACGGGGATAGTGAAGATGGCTG TCCTGAGAGGAAGGAATATTTATTATGTGACAGTGATAAATTGCCGCACTTGATTCTGGATTCTAGTAGC AAGATACGTGATTTGAATGCCAACACTGAATTGGAAGTAACGGAAGGTCAGAGTGTTGGTGTTCAAGGAG AAGCAGCGTGTATACAGATTGCGCAGTTAGATCTGAAGAACGTTTCTGATGGTGATAAATGGGAAGAGCC GTTTCCTGCATTTAAGTCTTGGCAGGAAGACTGTGAGTCTGGAGAAGCTCAGCTGTCTCCACAGGCTGCC AGGATGACTCACCACCCCCTGGGAGAGGACTGCCCTCCAGTATTATCTCATCGTAGTTTAGATTTTGGGC AAAGCCAACGTTTCCTACATGATCCAGAAGCGTTGGATTTCTCGTCAAAGGCACTTTCCTTCACTAGAAT TCGAAGGTCATCCTTCAGTTCAAAAGATGAGAAAAGAGAAGACAGAACACCATATCAGTTGGTCAAGAAA CTTCAGAAAAAAATCAGACAATTCGAGGAGCAATTTGAAAGGGAAAGAAATAGCAAGCCCTCCTACAGCG ACATTGCAGCCAATCCGAAGGTATTAAAATGGATGACAGAGCTTACGAAATTGCGGAAGCAAATTAAAGA TGCAAAACACAAAAACTCTGATGGAGAATTTGCGCCTCAGACACGTCCTCGGAGTAACACTCTGCCAAAA AGTTTTGGCTCTTCTCTAGACCATGAGGACGGCGAGAGTGAAGGTGAGCCCAGAGTCATTCAGAAAGAGA AGACGCCATCGAAAGAAGCAACTCTTGAGCTGATTACCAAACGGTTGAAGGAGAACCGTGCCGAGCGGCA CCTTCCTGAGGATATCAAGAAAATGACAAAAGATCATTTGATAGAAGAGAAAACGTCTCTGCAGAAAAGT CTGCTTTACTATGAAAGTCAACACGGAAGGCCGGTGACCAGGGAAGAAAGGCACATTGTTAAGCCTCTCT ATGATAGATACAGGCTTGTAAAACAGATGCTGACAAGAGCTAGCATTACTCCTGTCCTTGGTTCTCCTTC CACGAAGCGCCGGGGTCAGATGTTACAGCCAATCATAGAAGGAGAAACGGCACACTTTTTTGAAGAAATC AAGGAAGAAGAAGAAGATGGTGTCAGCCTCTCCTCTGAGTTAGGTGACATCTTGAGCACAAGCGTGCACA CACAGTCGTCCTTGGAAAACTTAGAATCTGATGCTGAAGAAAATCAAGAAAAACTGGCTCGGGATCTCTG CTTATCAAGTACCCGGGCAGCTTCTGTGCCTGAATTACTGGAACAACTTTGGAAAGCCAGAGCTGAGAAA AAGAAACTACGTAAGATGCTACGGGAGTTTGAAGAAGCGTTCTATCAACAAAATGGAAGGAATGCCCAGA AGGAGGATCGTGTTCCGGTGCTTGAGGAATATAAGGAATACAAGAGAATCAAAGCCAAGCTTAGACTTCT TGAAGTTCTTATCAGCAAACAAGACTCTTCAAAATCCATATAAAATATGTTCCTTGAAAGTTGACGCCAT AAAGTGCTTGTGTTCCCTGACAGTTCACCATGTGTGCTTGGCTGTACTTGCGTTCACTTTGTCAGGCACT CAGAGAGTCCCATTGTGTGCTTGTTTGTGGTTCCAGTAGGCAAGGAAAGCTGTTCCACATTCCCGCAGAT CCTCTCCACACTTAGGAAACACGCAGGCTCCTTCATTGTTTTACTGCAGACACGGCTGTTAGCAAAGGAC AAGAGTTTTACTCTTTTACTTCAAGAACTTTGAAATAAAAAGCTCTTCTGTTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3981146 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3981146 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004910 same experiment
	EMAGE:30083 same embryo
	EMAGE:30966 same embryo
	EMAGE:30201 same embryo
	EMAGE:30951 same embryo
	EMAGE:30984 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source