EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30264

2610312F20Rik ( MGI:1917711)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003526_01	euxassay_003526_02	euxassay_003526_03	euxassay_003526_04	euxassay_003526_05

euxassay_003526_06	euxassay_003526_07	euxassay_003526_08	euxassay_003526_09	euxassay_003526_10

euxassay_003526_11	euxassay_003526_12	euxassay_003526_13	euxassay_003526_14	euxassay_003526_15

euxassay_003526_16	euxassay_003526_17	euxassay_003526_18	euxassay_003526_19	euxassay_003526_20

euxassay_003526_21	euxassay_003526_22	euxassay_003526_23	euxassay_003526_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7420
Entity Detected:	2610312F20Rik, ( MGI:1917711)
Sequence:	sense strand is shown >T7420 CCCACGCGTCCGCGCGGAAGTTCAGTGAGAAGATCGCCCTGCACACGCAGCGGCAGGCCGAGGAGACGCG GGCCTTCGAGCAGCTCATGACCGACCTCACCCTGTCCCGGGTTCAGTTTCAGAAGCTTCAGCAGCTGCGC CTTACCCAGTACCACGGGGGATCCTTACCCAACGTGAGCCAGCTGCGGACCAGCGCGCCAGAGTTCCAGC CATCACTTCATCAAGCCGATAATGTTCGTGGAACCCGCCACCATGGGCTGGTGGAGAGGCCAGCCCGGAA CCGCTTCCACCCCCTTCACCGAAGGTCTGGGGACAAGCCAGGGAGACAGTTTGATGGGAATGCTTTCGCA GCCAGTTATTCTTCACAGCACCTGGATGAGAGCTGGCCACGGCAACAGCCTCCTTGGAAAGAAGAGAAGC ATCCTGGATTCAGGCTAACATCTGCACTTAACAGGACCAATTCTGATTCTGCTCTTCACACGAGTGCTCT GAGCACCAAACCCCAGGACCCCTATGGAGGAGGAGGCCAGTCAGCCTGGCCTGCCCCATACATGGGTTTC TGTGATGGTGAAAACGACGGCCATGCGGAAGTAGCCGCCTTCCCGGGTCTGCTGAAAGAAGAGAATCTGT TAAATGTCCCGAAGCCACTGCCTAAACACCTGTGGGAGAGCAAGGAGATCCAGTCCCTGTCTGGGCGCCC TCGATCCTGTGATGTTGGGGGCGGCAATGCTTTCCCACATAATGGCCAGAACACAGGCCTCTCACCGTTC CTGGGCACCCTGAACACCGGAGGGTCGTTACCAGATCTAACCAACCTCCACTACTCAGCACCCCTGCCAG CCTCCCTGGACACCAGCGACCACCTCTTTGGTAGCATGAGTGTGGGGAACAGCGTGGGCAACCTTCCAGC TGCCATGACTCACCTGGGGATAAGAACCTCCTCTGGTCTCCAAAGTTCTCGAAGTAACCCTTCCATCCAA GCCACACTCAGTAAGATGGCACTCTCCTCGTCCCTCAAGTGCCACCCGCAGCCGTCTGTGGCCAATGCCT CTGCTCTGCACCCCTCCCTCCGGCTCTTCTCCCTTAGCAACCCGTCTCTTTCCACCACAAACCTGAGTGG CCCCTCTCGGCGCAGGCAGCCTCCAGTCAGCCCTCTCACGCTCTCTCCTGGCCCTGAAGCACATCAAGGC TTCAGCAGACAGCTCTCTGCGACCAGCCCACTGAACCCGTATCCTGCCTCCCAGATGGTGACCTCAGAGC AGAGCCCACTTTCCTTCCTGCCCACAGACGCTCAAGCCCAAGTGTCCCCACCACCCCCTTACCCCACACC CCAGGAGCTGCCTCAGCCACTCCTACAGCAGCCCCATGCCCAGGAGCCCCCAACTCAGCAGCCCCAGGCA GCCCCCTCCCTGCCACAGTCAGACTTCCAGCTCCTCACTGCCCAGGGCTCAGCTTTGACCAGCTTCTTCC CGGATGTGCGCTTTGACCAACAGCCCATGAGGCCCAGCCCTGCCTTTCCTCAGCAGGTGCCTTTGGTACA GCAGAGCCACCGAGAACCACAGGACTCATTCCACTTGAGACCAAACCCATATTCCAGCTGTGGCAGCTTC CCAGGCACCATCCTGACAGAAGATACAAACAGCAACCTGTTCAAAGGCCTTAGCGGGGGGCTGTCGGGCA TGCCAGAGGTCAGCCTGGACATGGACACCCCGTTCCCTCTGGAAGAGGAGCTGCAGATCGAACCCTTGAG CCTCGATGGACTCAACATGCTAAGTGACTCCAGCATGGGCCTGCTGGACCCCTCTGTGGAGGAGACGTTT CGAGCTGACCGGCTATGAGCACAGTGCAGTGGAACTGGGGAACTGAAATGGCCAGAGAAAGCTGGGGTGG AGCCAGCAAACACCCTGCCGGTGGTCACAGCCAGCAAACACCCTGACAGTGGTCACAGCCAGCAAACACC TTGACGGTGGTTACAGCATAGAGGGAGCCGCTGCCCCGGGCTCTCCACTGAGCTCAGCCAAGGCTTTGCT AGACACGGCTGCACTCTTCGGCCCATGGCACCAGTTGTACTGCTGCAGGCCAGCTGCTTTGGAGCTTCCG GTGACCAGGAAGCTGACCATGTCACCCTTAATTCTCTTTATTGGCCATCCAGTCAGTCAGTGTGTAAATG TAGAAAATGCTGTGTAACACGTCAGTGCTCCAAACCTCAGATTCCCATCAGCACTGAAGATGGAACGCTA TAGGAGGCAGTTGGGGGCCTCGGGCCCGTGTAGTTGGCTCAGTGTCACACAACGGTACCAGAGCACCATG ATGCCTTTCTGTCTGACCGCCAAGGCCAGGCAGCCCTCCAGCTGGCGGGTATTCTGGGCCCAGCCATCAC TCTGTCCAATGGCTCACTTAAAATGAAGAAGCTTACCCAGCCGTCCCAGGGCAGGCTTAAGTTAGGCAAA CTGAAGGCAGTTTGCTTCCTCCCGGTTTATGATCTCTTTGCTGCTGCTTCCTGTATGCGCACCTTTCCCT CACAGACAGGAGGAGAGGGAGACCTGTTTGCTCACTGAAAAAAATGTCCTTGGAAACAGGATGAGCCTAA GGAGGACAGCGCTCCTAGGAGAATGGAGGAGGAAAGGGAGGCTACAGGTGTGTCACCTGCACAAGATGCC CTCCCTCTTAGGCCTGCCACCCTCCAGCCCACTGGAAGGAGCTGCTGGGACTCGTATTAGTGCAGGGCAG CTGAACTGCCCTTCTCCCCAGCTGCCCTGCAGACCTCAAGTTTAATGCAATGCCCACTAGACTGTAAGCT CCCAGAGGGCAGGAACTGTGCCTTGTTCTGTATTGACCCCTAGTGCCCACCAAGTGCCTGGTAGGAGTGC ATGTACCCAGGGATGGAGGAGAGAGGGACTGTGCCTCGTGGTGGGGCGGTCTAACCTGCAGCTCTCTCTG TCCCCTGCCTTAGCTCAGAGCTTCACCTTTCAGACTCACTTGAGTGTGCCTTAACACTAGGTGACCAGAC CTGCCACTAGGCTCAGCGCCACTCCTCCTAGGGAATATGTCCCTCAGCAGCTAGGTGGACATCATCCAGT GCATCCAGCATGGCTGATCACACCAAGCCCCAGGAGTGTGGGGCACCTGGCATCCAGACTGGGCCCCTAC AGCAAATTCTTCCTCTCGGACCTGTACTTTTTTTTTTTCTAAATTGCTTAAAATAGAGGTGCAAACTTTG AGGTTAAGAAATAAGGCCTGGCTGGGCGTGGTGGCACCCGCCTTTAATCCCAGCACTTGGGAGGCAGAGG CAGTAGGAATTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGATAGCCAGGGCTACGCAG AGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAACAAAAAAGAAAGAAAGAAAGAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4195538 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4195538 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003526 same experiment
	EMAGE:30168 same embryo
	EMAGE:30199 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source