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EMAGE:30338

Nek8 ( MGI:1890646)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50735
Entity Detected:Nek8, ( MGI:1890646)
Sequence:sense strand is shown

>T50735
CGCACGCAGAAGGCTGGCGTTACGCGCTCTGGACGCCTCATCCTGTGGGAGGCCCCACCCCTGGGAGCTG
GAGGCGGCACACTCCTTCCTGGAGCCGTGGAGCTGCCTCAGCCCCAGTTTGTCTCTCGTTTCCTGGAAGG
CCAGTCAGGTGTGACCATCAAGCATGTGGCCTGCGGGGACCTGTTCACCGCTTGCTTGACAGACCGAGGC
ATTATCATGACGTTCGGCAGTGGCAGCAATGGGTGTTTAGGTCACGGCAACCTGACTGACATCAGCCAGC
CCACCATTGTAGAAGCCTTGCTGGGCTATGAGATGGTGCAGGTGGCCTGTGGGGCCTCTCATGTGCTGGC
CCTGTCCACAGATGGAGAGTTATTCGCCTGGGGCAGAGGAGATGGTGGTAGACTGGGACTAGGCACCAGG
GAGTCCCATAACTGCCCTCAGCAGGTGCCTGTGGCCCCAGGACAGGAAGCTCAGAGAGTTGTCTGTGGTA
TTGACTCTTCCATGATCCTCACCTCTCCAGGCAGGGTCCTGGCCTGTGGAAGTAACAGGTTCAACAAGCT
AGGCTTGGACCACCTATCCCTGGACGAGGAGCCTGTTCCCTACCAGCAAGTGGAGGAGGCCCTGAGCTTC
ACACCACTGGGCTCTGCACCTCTGGACCAGGAGCCCCTGCTGTGTGTGGACCTTGGCACTGCTCATTCAG
CTGCTATCACTGCTTCTGGGGACTGCTATACTTTTGGCAGCAATCAACATGGACAGCTGGGCACCAGTTC
CCGCAGGGTCAGCAG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. .
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_018630 same experiment
 EMAGE:29582 same embryo
 EMAGE:29608 same embryo
 EMAGE:30364 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS