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EMAGE:30396

Depdc5 DEP domain containing 5 ( MGI:2141101)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30396 EMAGE:30396 EMAGE:30396 EMAGE:30396 EMAGE:30396
euxassay_001934_01 euxassay_001934_02 euxassay_001934_03 euxassay_001934_04 euxassay_001934_05
EMAGE:30396 EMAGE:30396 EMAGE:30396 EMAGE:30396 EMAGE:30396
euxassay_001934_06 euxassay_001934_07 euxassay_001934_08 euxassay_001934_09 euxassay_001934_10
EMAGE:30396 EMAGE:30396 EMAGE:30396 EMAGE:30396 EMAGE:30396
euxassay_001934_11 euxassay_001934_12 euxassay_001934_13 euxassay_001934_14 euxassay_001934_15
EMAGE:30396 EMAGE:30396 EMAGE:30396 EMAGE:30396 EMAGE:30396
euxassay_001934_16 euxassay_001934_17 euxassay_001934_18 euxassay_001934_19 euxassay_001934_20
EMAGE:30396 EMAGE:30396 EMAGE:30396 EMAGE:30396
euxassay_001934_21 euxassay_001934_22 euxassay_001934_23 euxassay_001934_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3982
Entity Detected:Depdc5, DEP domain containing 5 ( MGI:2141101)
Sequence:sense strand is shown

>T3982
TTTTTTTTTTTTTTGAGGGAGGGAGGGCTTTATTAGATTCTGCTATGTACACATATGTACAGCTGGTGTG
AGCCAATGGTACATTATGCCACAGATTTACCTCATACACACACTCTTGGGCAGAAAGCAGGAGGTTCTGT
TCCGAATATCCCACTGCTGGTGAAGGAAAAGGACCCTAATGGAAGCTGCCTACCCCACTCCCAGACACTG
ACTCACTCCCACGGACAATCTGTGCCTCCCCCTGCTTCAGGGACAGGATTGGCAAGATTTGGTTGGCGGT
AGCGGCAGCAGCTGCTTCCAATCTTTCTCTTCACTCACACTTGTGCAGAGGCCAAATATCAGCAGTGGCC
CCACTCACTGATAGGAGCAGCCTGACACCTGCTGCCCAGAGCCTGACCGGAAGGCCAGCTCCAGCATACC
CACAGCCTCTCTTAAGAGAAGCAGCCTGGCTTCATGGAGCACTGGCATGCATCTTCTCCAGGCAGTTTGT
CCAGAAC
Notes:The probe template was PCR amplified from IMAGE:865305 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:865305 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001934 same experiment
 EMAGE:29507 same embryo
 EMAGE:30421 same embryo
 EMAGE:30423 same embryo
 EMAGE:30400 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS