Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30668

Trim6 ( MGI:2137352)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30668
euxassay_003612_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6299
Entity Detected:Trim6, ( MGI:2137352)
Sequence:sense strand is shown

>T6299
GAATTCAGTAATTTTTTTTCTCTAGTTACTGAGATACTTGTGTATCCTTTCTATTCTGCTCCTGGGTCAG
CCTCACTGAGTTGTCTCAGTTGTTACGTTTACCACTGATTCCTGAAGCAAAACTGTCATGTGTTACATTC
AGCACTTTATGTTTGCTGACTCTAGGTTTGGTTTTTGTCTCTTTATAAACAAACTACCTCTAAGTTTGCA
TTCTTTGTTATCTGTCAAGGCATATCAGAAAGAAATTATGGGGAAGGGCTTATGTATGTGTATTTAGGAT
CACAATAAATCCTAAGTATGTATATTTAGGATTACAGTAAAAATAATAAAATCTTTGGATGGTTTCAAGA
AGAGATTGTAGCTTTGGTTTTAACAATAAATGCTACCACAACCTTCAGCCTTCAGCATAAAATTTTAAGA
GTGGTAATAAAAACTAGAAAATGCTCTTGAAAGGGAGATAAAGTAAGATAAGGAGAAGACTTCAATCAAC
CAGTATCAGTTATTATTTAGGAGGAGGAAGAAATTCAGTTACCCATTCTCACAGTGAGTTATTCTGGTCC
CCCCAGCCCCTCTTATTTCTTCTGTAGAGCTAG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers.. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003612 same experiment
 EMAGE:29628 same embryo
 EMAGE:32105 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS