EMAGE mouse gene expression spatial database

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EMAGE:30754

1700102P08Rik ( MGI:2148248)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_005894_06	euxassay_005894_07	euxassay_005894_08	euxassay_005894_09	euxassay_005894_10

euxassay_005894_11	euxassay_005894_12	euxassay_005894_13	euxassay_005894_14	euxassay_005894_15

euxassay_005894_16	euxassay_005894_17	euxassay_005894_18

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9792
Entity Detected:	1700102P08Rik, ( MGI:2148248)
Sequence:	sense strand is shown >T9792 GGGCACTCTTGTGCGGCAACGGCTGGGGAAATGTCTGAAAAACTACGAAGATGCAGAAAGGAGCTGACTG CTGCTATAGACCGAGCCTTTGAAGGAGTCAGGCATTCTCAGGAGTGCACAGCCCAGCAGAGGCTGGACGC CCCGTCGCTCACCTCCCAGCCGGTACACAGGCTCCTCTGCAGAAACCCACTGGCTGCCTGCCCCTCTGCT GCCCCATACTCTGGTGCCTCGTGTGCTCCTGAGAGTGAGAACCCGGCCTTCGGGACACACCATATTCCGG TTAATTCAAAACTTCAGCAGCCTCTATACCCCAAAAGGAAACCTCTGACCAGCAAGGAAAATGTCTTGAT GCAGTCTTCCATTTTGGCACGTGACAGACAGTTTTGGAGAGCTGCAGGTGATGGGGAAGACTGGAGAAAA GATAGTTTAAGGAAGGATATGGAGAGAGATTTAAAAGCTGACCCAAATGTACTGCTCAGCAGTTCTAGCC AAGAGGTCACAAAGGATCTGCTAGACATGATTGACCATACAAGTATCCGAACTATTGAAGAATTGGCTGG AAAACTAGAATTTGAAAATGAATTGAACCGTGTGTGTGGACACTGCCAAGATTCACCCTTCAAGGAGGAA GCCTGGGCCCTGCTTGTGGATGAGAGTCCTCAGAAGGCTCTGGATGCTGACCCTGGTAGCCTCAAGCAGG CTTTGGATGATCAGAATATAGTTGAGACTGTTCTGGACTTGGAAGAAGACTACAACTTGATGACTTCTTT TAAATACCAAATAGAGTGAGGAGAGTGGATGTCAGCAGCCACAGTGGTTACCAGAACAAGTCTTCACGAG GGGTTGGCAGAGCCCAAATTGCCACACCTTGTTTAATTTCTGCTGAATCCCATTTTGAGCTGAGAGCCCA GGTTTTAGTTCTGTTATTTTGAAATGTTTGAAAAGTTTTTTTTGGACTTGACATTTTTTTTAAAGTGTAT TCTTGGTTAGTGTTGAATATATACAGTCCCCAGGATAGGTTATAGGTTGTGCCTCTGCTCTTTGTCCTTG TATTCTTTTATATGAATCCCTGGAAAAATAAAAATCCAGGAACCATTAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6770968 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6770968 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005894 same experiment
	EMAGE:31698 same embryo
	EMAGE:31687 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source