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EMAGE:30804

B3gat1 ( MGI:1924148)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30804 EMAGE:30804 EMAGE:30804 EMAGE:30804 EMAGE:30804
euxassay_012950_01 euxassay_012950_02 euxassay_012950_03 euxassay_012950_04 euxassay_012950_05
EMAGE:30804 EMAGE:30804 EMAGE:30804 EMAGE:30804 EMAGE:30804
euxassay_012950_06 euxassay_012950_07 euxassay_012950_08 euxassay_012950_09 euxassay_012950_10
EMAGE:30804 EMAGE:30804 EMAGE:30804 EMAGE:30804 EMAGE:30804
euxassay_012950_11 euxassay_012950_12 euxassay_012950_13 euxassay_012950_14 euxassay_012950_15
EMAGE:30804 EMAGE:30804 EMAGE:30804 EMAGE:30804 EMAGE:30804
euxassay_012950_16 euxassay_012950_17 euxassay_012950_18 euxassay_012950_19 euxassay_012950_20
EMAGE:30804 EMAGE:30804 EMAGE:30804
euxassay_012950_21 euxassay_012950_22 euxassay_012950_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
spinal cord mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
pons mantle layer
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38870
Entity Detected:B3gat1, ( MGI:1924148)
Sequence:sense strand is shown

>T38870
AGTCTTCAGGACACCACCTGATGGGATCAGACCCCACAGCCCCCTGTAGGACATCTCCCCCTAGCCATTT
ATACTTTGGATATTGTTTTGGAAATTTACTCCTTGGCATCAAATGAGAGGCACCGGGAATTTATCCGATG
GGTGAATTTATGTCTGGGCACCCTACTGCTCATTCATAGAAACTGTCAGTGAATGCCAAGGTCAAACGGG
CTGCTAAGGCTGCCTTCTTAGCCAAACTCAGTGCTCTTTTTAGGAAACTGAAAAAAAACAAAACAAAACA
AAACAAAAATGGGGATTTTTACTTTTCACGTTTTGGCAGATGCTACTTCAGTTGTTTTCATGTAATGTCC
TCCCTTCACTGCATGTGTATTTATTTAAAATTATAACCCTGGTGGACTGCGGCCTTCATCTCTGTTGGGA
ATGAGTTTGTTGCAAATCAGAACTGGGTCCATGATGACAACTTTCCCTCCAAACCCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 178521. Forward Primer - name:178521_F_cDNA_B3gat1, sequence:AGTCTTCAGGACACCACCTGAT; Reverse Primer - name:178521_N_SP6_cDNA_B3gat1, sequence:CTGGGTTTGGAGGGAAAGTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012950 same experiment
 EMAGE:29959 same embryo
 EMAGE:30162 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS