EMAGE mouse gene expression spatial database

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EMAGE:30830

Lrrc5 ( MGI:1922368)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006270_01	euxassay_006270_02	euxassay_006270_03	euxassay_006270_04	euxassay_006270_05

euxassay_006270_06	euxassay_006270_07	euxassay_006270_08	euxassay_006270_09	euxassay_006270_10

euxassay_006270_11	euxassay_006270_12	euxassay_006270_13	euxassay_006270_14	euxassay_006270_15

euxassay_006270_16	euxassay_006270_17	euxassay_006270_18	euxassay_006270_19	euxassay_006270_20

euxassay_006270_21	euxassay_006270_22	euxassay_006270_23	euxassay_006270_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 10 moderate expression: see section 19 20 21 22

metanephros

moderate

regional

moderate expression: see section 04 05 06 07 08 weak expression: see section 09 10 15 16 17 18 19 20 21

spinal cord

weak

homogeneous

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 19 20 21 22 23 24 weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 09 moderate expression: see section 21 22

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 08 moderate expression: see section 23 24

lower jaw molar

moderate

regional

moderate expression: see section 08 21 weak expression: see section 09 22

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 11 12 13 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 10 11 moderate expression: see section 19 20

upper jaw molar

moderate

regional

moderate expression: see section 08 21 weak expression: see section 09 22

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 moderate expression: see section 19 20 21 22 23 24

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 14 17

vibrissa

weak

regional

weak expression: see section 05 06 07 08 09 10 11 21 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 15 16 17 18 19 20

lower jaw incisor

weak

regional

weak expression: see section 12 13 14

upper jaw incisor

weak

regional

weak expression: see section 12 13 14

lung

moderate

regional

moderate expression: see section 03 04 22 23 24 weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7519
Entity Detected:	Lrrc5, ( MGI:1922368)
Sequence:	sense strand is shown >T7519 CCACGCGTCCGGCTCTTGGGAACGGGACTTCTGCTTGATTCTGGTTTAGGGGCTTGAAAAAGAGGAGAAC TGGGGTGCCTTCCCAGAGGACTGTCGCCTCCAGTTACAGTCGATGGGGGACACTCTGTCTCTGTCCCCCT TTATGTGGGGTATCCCTAACAGATAAGGAAATGAGATTTTTTTGTTGTTGTTCTTTGATTTTTTTTTTTA AGCCATCCGCAGTGAAGGAGAACTGCTTCGGGTCGAGGGGAGGAGAGGAAGTGCACTGCTGACTGCAACG TGCTGCCGGCTCCCAGGATGGAGGAGTGAAGTCTCGCGTCGCAGCGGTTCCAGCCCCCGGAGCCTGCCCG CAGCCGAGTCCTCGAGAGCGCCCTGAGGGAGCAGGGTGGCCACCTGGTCCAGGAATGTTTACCCTTGCGG AAGTTGCTTCACTTAATGACATTCAGCCAACTTACCGAATCCTGAAGCCATGGTGGGACGTGTTTATGGA TTACCTGGCAGTCGTTATGCTGATGGTAGCCATCTTTGCAGGGACCATGCAACTTACCAAAGATCAGGTG GTCTGTTTGCCAGTGTTGCCGTCGCCTGCAAATTCAAAAGCGCACACACCACCCGGAAATGCTGACGTCA CCACCGAAGTCCCCAGGATGGAAACAGCCACACACCAAGACCAAAACGGGCAGACGACAACGAATGACGT TGCCTTTGGCACATCCGCTGTGACCCCTGACATACCTCTCCAAGCCACCCATCCTCATGCAGAGTCCACC CTTCCCAATCAGGAGGTGAAGAAGGAGAAGAGAGACCCAACGGGCCGAAAAACCAACTTGGATTTTCAGC AGTACGTATTTATCAATCAGATGTGTTACCATCTGGCCCTCCCTTGGTACTCCAAGTACTTTCCATACCT TGCCCTTATACACACCATCATCCTTATGGTCAGTAGCAACTTTTGGTTCAAATATCCCAAAACGTGCTCC AAGGTTGAGCATTTTGTTTCAATATTAGGAAAGTGTTTCGAATCTCCCTGGACAACTAAGGCGCTGTCAG AGACGGCCTGTGAAGACTCTGAAGAGAACAAGCAGCGGATAACGGGCGCCCAGACCCTGCCGAAGCATGT GTCCACCAGCAGCGATGAGGGGAGCCCCAGCGCCAGCACCCCCATGATCAACAAAACTGGCTTCAAGTTC TCTGCTGAGAAACCGGTGATCGAAGTTCCCAGCATGACAATCCTGGACAAGAAAGACGGGGAGCAGGCCA AAGCCCTGTTTGAGAAAGTAAGGAAATTCCGTGCCCACGTGGAAGACAGTGACTTGATCTATAAGCTCTA TGTGGTCCAAACGCTCATCAAAACTGCCAAGTTCATTTTTATCCTCTGCTACACTGCGAACTTTGTCAAC GCCATCAGCTTCGAGCACGTCTGCAAGCCAAAAGTCGAGCACCTCACGGGGTACGAGGTGTTCGAGTGCA CTCACAATATGGCCTACATGTTGAAAAAGCTGCTCATCAGCTACATCTCCATCATCTGCGTCTACGGCTT TATCTGCCTCTACACTCTCTTCTGGCTATTCAGGATCCCTCTGAAGGAGTACTCGTTTGAGAAAGTCCGG GAGGAGAGCAGCTTCAGCGACATCCCGGATGTCAAGAACGACTTTGCGTTCCTTCTGCACATGGTCGACC AGTACGACCAGCTCTATTCCAAGCGTTTCGGTGTGTTCTTATCCGAAGTCAGTGAGAACAAACTGAGGGA AATCAGCCTGAACCATGAGTGGACTTTCGAGAAACTCAGGCAGCACGTGTCCCGCAATGCCCAGGACAAG CAGGAGCTGCACCTCTTCATGCTGTCCGGGGTGCCCGACGCTGTCTTTGACCTCACAGACCTGGATGTGC TAAAACTTGAACTGATCCCCGAAGCAAAAATTCCTGCCAAGATCTCTCAGATGACTAACCTCCAAGAGCT CCACCTCTGCCACTGCCCTGCAAAGGTGGAACAGACTGCCTTTAGCTTCCTCCGAGATCACTTGAGATGC CTTCACGTGAAGTTCACGGATGTGGCTGAAATCCCCGCCTGGGTGTATCTGCTTAAAAACCTGCGGGAGC TGTACTTGATCGGCAATTTGAACTCGGAAAACAATAAGATGATCGGCCTTGAGTCGCTGCGAGAGCTGCG GCACCTTAAGATCCTCCACGTGAAGAGTAATCTAACCAAAGTCCCCTCCAACATCACCGACGTGGCTCCG CACCTCACGAAGCTCGTCATCCACAACGACGGGACCAAGCTCTTGGTCCTGAACAGCCTGAAGAAGATGA TGAACGTGGCCGAGCTCGAGCTGCAGAACTGTGAGCTGGAGAGGATCCCACACGCCATCTTCAGCCTCTC TAATCTGCAGGAGCTGGATTTAAAGTCAAACAACATCCGGACGATCGAGGAGATCATCAGCTTCCAACAT CTGAAGCGACTGACTTGCTTAAAGCTGTGGCACAATAAAATTGTGGCCATCCCACCCTCCATTACCCATG TCAAGAACCTGGAGTCCCTCTACTTCTCCAACAACAAGCTCGAGTCTTTACCGACGGCAGTGTTTAGTTT ACAGAAACTCAGATGCTTAGACGTCAGCTATAACAACATTTCCACGATCCCCATAGAGATAGGTTTGCTC CAGAACCTGCAGCATTTGCACATCACAGGGAACAAGGTGGACATTCTGCCAAAACAGTTGTTTAAGTGCG TGAAGTTGAGGACTTTGAACCTGGGGCAGAACTGTATCGCCTCCCTGCCTGAGAAAATCAGTCAGCTCAC CCAGCTCACTCAGCTGGAGCTGAAGGGCAACTGCCTAGACCGCCTGCCAGCCCAGCTGGGCCAGTGTCGG ATGCTCAAGAAGAGCGGGCTTGTTGTAGAAGACCAACTGTTTGACACGCTGCCACTAGAAGTCAAAGAGG CATTGAATCAAGATGTCAATGTCCCCTTTGCAAACGGGATTTGAACTAAGCGAATGCAGGCACACCCACG TGCGGGGGGGACACCTTCCTAGATTGCAGATGCTCACTTGCAAGTTATTACAAGATAATGCATTTTAGGA GTAGATAAGCCCTTTAAACAGGACACGATGCATAGAGGCTGGTAGCAGACGAAACCGAATGTTCCATGTT TGTAGCATTTTAAGTCATTCATTTCCAGATCTTTTCTTTACTTTTTGTGGGTTGTTTTTTTGGGGGGGGG GTTGTTTCCTCTTCCTTCTCTTCTTCTTCCTCTTCCTCCTCCTCCTCCTCCTCTTTCTGGGGAAAGGGAA GGAAAAATCACAATCACTGATCTCAGTTCTTTTTAAATTGTAAGTCGGATGCGGCTGCTGGCTGAGTGTT TACACATTGCTTGCCTGCTAAAGTATATGATTAAACTGACTTTCTTCTTACTACATTGCAGTTTTTTGGT TTTTTGGTTTTTTTCTTTCTTTCTTTCTTGAGGTGTCCTGATTTATTTTTGCTTAGCTGGTGCAATATTG CTTAACCTTGGATCGTTGAAGACACAAGGGGCCAAACTCATAATCTTAGATTTGCTAAAATATTGACTTC TTTACTGTTTTAACTTAAAGCAAGTGAGTGGAGCTTCCTGGAGCATTTGATGGTACTGTACCTCTGCGAA TCCCACAGCAGACTTTACAGTCTTGGCGGCCTGGAATGAAGTAGAGCGTGTGGTGTGTCAAGACTCTTTG ATTGTTTCTTAAAACAAATACTAAAAATAAAATAACACATCCTTTAATAGTCACTTTATGTGCATAGGCT GGAGTTTTGGTTATACATGGTACACTTTTGGGCCAATATAATAGGATTTCCAATGAATAGAAAATGTGTT TTAAAAATAAATTTATAACAATAAAATACGTTTATCTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4239389 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4239389 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006270 same experiment
	EMAGE:31554 same embryo
	EMAGE:30158 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source