EMAGE mouse gene expression spatial database

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EMAGE:30875

BC024139 cDNA sequence BC024139 ( MGI:2442591)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009734_01	euxassay_009734_02	euxassay_009734_03	euxassay_009734_04	euxassay_009734_05

euxassay_009734_06	euxassay_009734_07	euxassay_009734_08	euxassay_009734_09	euxassay_009734_10

euxassay_009734_11	euxassay_009734_12	euxassay_009734_13	euxassay_009734_14	euxassay_009734_15

euxassay_009734_16	euxassay_009734_17	euxassay_009734_18	euxassay_009734_19	euxassay_009734_20

euxassay_009734_21	euxassay_009734_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16

corpus striatum

weak

regional

weak expression: see section 01 02 03 04 05 19 20 21 22

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

pons ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13

spinal cord ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30586
Entity Detected:	BC024139, cDNA sequence BC024139 ( MGI:2442591)
Sequence:	sense strand is shown >T30586 GCCCAGCTTGAACTGGAAGGCTCTGCCTCAACACCTCCACAGCTCAAGGATCTGAAGCAGCTGAGTGTCC AGCTCGTCCAGTTAGCAGAACGACTTGAGCAGCTGGCTCAGTGGGCAGAGACTTCTAGTCAGGTAGCAGC ACCCGCAGTGACCATCTGGGAACAGAGTCTCTCATCAGCCATGCTTCAGGCCGATACGAAGCCCCTGGAA GGGCACTGGTTAGAGACTCGAGAACAAGACTTTGGGCCGAAGGCCATATGGGAGCCAGCTGTGCAGATCT CATGTGGCCTGGCAGATCAGGCAGCCATTGAGAAGCTAAAGCCTGAGAGGGACTGGAACCCAAGTTTACA GGAGTTCCTGTGGGAACAGCAAACCCTGGCAGTGTCATGGCTAGGAAGGAGAGGAGCTGCTGACTATGGG AGCACCTGCCACTTGGGCCTGCTGATGGACCTGCAGGGCTGCCGGGCTGCCATGTCTAGCCCCTCCTGCG AGAGGATACACACCCTGGAAGGGGTGCTGCTGCTTGTGGGCCACCTTGTAGAGAAGTTGGTCTTGTGCCC CCATAGACTGAGCCAAGCGGAACCCTTGGGGATCAAGGTGGTACAAGCCCACAGCTTGTTAGAGGAGATG CCTCCGGCCCTCACCCCAAAGTACTGGACTCGAGCCCCAAGGGAGATACTGGCTTTGATGAGTCTTGACC AGGAGCCGCCTGGGGATCTGGCGAGTAGAGCTAAGGCTTGCCTCCTGGGGAAGATTCCAAAAGAACTGAG CTGGAGGCCATGGTGGTCAGAGGTCATGGAGAACTGGAGTCTTAGGAAGCCAGAGCATAGCAAGAGGAGC GAGGACAAAAGGGACCAGTGGGAACGGCTGGAACAGGGCCACCAGGTAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5694464), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59901. Forward Primer - name:059901_F_IRAV113_f03_BC024139, sequence:GCCCAGCTTGAACTGGAA; Reverse Primer - name:059901_R_SP6_IRAV113_f03_BC024139, sequence:TGTACCTGGTGGCCCTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009734 same experiment
	EMAGE:30868 same embryo
	EMAGE:30010 same embryo
	EMAGE:30013 same embryo
	EMAGE:30027 same embryo
	EMAGE:30015 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source