EMAGE mouse gene expression spatial database

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EMAGE:30884

9530091C08Rik ( MGI:2444034)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006470_01	euxassay_006470_02	euxassay_006470_03	euxassay_006470_04	euxassay_006470_05

euxassay_006470_06	euxassay_006470_07	euxassay_006470_08	euxassay_006470_09	euxassay_006470_10

euxassay_006470_11	euxassay_006470_12	euxassay_006470_13	euxassay_006470_14	euxassay_006470_15

euxassay_006470_16	euxassay_006470_17	euxassay_006470_18	euxassay_006470_19	euxassay_006470_20

euxassay_006470_21	euxassay_006470_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 06 15 moderate expression: see section 07 08 10 13 14 not examined expression: see section 09

adrenal medulla

weak

single cell

weak expression: see section 06 07 13 14

midbrain mantle layer

strong

single cell

strong expression: see section 06 07 08 moderate expression: see section 10 13 14 15 16 17 weak expression: see section 09

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20

pons ventricular layer

strong

single cell

strong expression: see section 05 16 17

pons mantle layer

strong

single cell

strong expression: see section 05 06 16 17 18 moderate expression: see section 07

thalamus mantle layer

strong

single cell

strong expression: see section 15 moderate expression: see section 08 09 16

dorsal grey horn

moderate

single cell

moderate expression: see section 07 08 13 14 weak expression: see section 09 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35787
Entity Detected:	9530091C08Rik, ( MGI:2444034)
Sequence:	sense strand is shown >T35787 TGACATCTCTCTAGGCTCTCCCTCTACAAAGCCTTCCCTGAGCCCACCACTGCTCACCCCACCCCATCCC CCACCTCTGTGCCTAGATTCCGGCATCTGTGCAATGGAGTTACAGAGCTGTTCCATTTTAAGTCCTCAAA AAAGTACAAACGAGATTGAGAAACTTATCTGAGAGGAATTTTGAAAGGTCTGCTCCAAACTGTTTAGCTA TTTTTCCTAAGAGCCAGAAGCACAAAAGAACACACTCATTATCCCATGGCTTCCCAGATCTCACCATGAA AAATAATCGCTGTCAGATTCTCTGGGCTTTCGGTTCATGGGGCTCTTTCTTAAGGAGGAAACGGGACTGG AGTCCTGGCTCACAAGAAACATCACCCTTTCCACCTACACCCTGTTCCTCACTCCATCCACAGCATCTGT TTATCTCCTGTAGGGTTATGCTAACACTACACACACTGTAGGATGATTGGCACTGCCTTCTCTCCCCTTC GCTCTGGGCTCGTTGAGAGGAGAGACTGTATTCTAGTGTGTGCCCAGCCTGGTTCCTTAACCTCAAAGTT ACAGTGTCTTTATTGAATGAGAAAATGAGTGCTTGAAGAAACAATGAAGGAGGTTTTATGGTTATCAAGC AGAAGAGACTAGAGCCCGGCTGTTTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 69617. Forward Primer - name:069617_F_cDNA_9530091C08Rik, sequence:TGACATCTCTCTAGGCTCTCCC; Reverse Primer - name:069617_N_SP6_cDNA_9530091C08Rik, sequence:GAAACAGCCGGGCTCTAGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006470 same experiment
	EMAGE:30889 same embryo
	EMAGE:31360 same embryo
	EMAGE:29484 same embryo
	EMAGE:29234 same embryo
	EMAGE:29245 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source