EMAGE mouse gene expression spatial database

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EMAGE:30904

Ahi1 ( MGI:87971)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005590_01	euxassay_005590_02	euxassay_005590_03	euxassay_005590_04	euxassay_005590_05

euxassay_005590_06	euxassay_005590_07	euxassay_005590_08	euxassay_005590_09	euxassay_005590_10

euxassay_005590_11	euxassay_005590_12	euxassay_005590_13	euxassay_005590_14	euxassay_005590_15

euxassay_005590_16	euxassay_005590_17	euxassay_005590_18	euxassay_005590_19	euxassay_005590_20

euxassay_005590_21	euxassay_005590_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

weak

single cell

weak expression: see section 02 03 04 05 06 16 17 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 12 15 16 17 weak expression: see section 11

cervical ganglion

moderate

regional

moderate expression: see section 06 weak expression: see section 13

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 06 10 11 12

spinal cord

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

glossopharyngeal ix ganglion

weak

single cell

weak expression: see section 05 14 15

trigeminal v nerve

weak

single cell

weak expression: see section 08 09 16

thoracic ganglion

moderate

regional

moderate expression: see section 07 08 09

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5545
Entity Detected:	Ahi1, ( MGI:87971)
Sequence:	sense strand is shown >T5545 AGCACGTGAAGATTCAGAGGACAATGTTAGTGTAGAGCCATACAAACAAGAAAAGACAAGTAAACACTAC CCAGATCGCACAGGAAATGGAGCCAGAAACTCCAGAGAAGGTGGATTCTGCACAAGAGAAAGTCAGAGGC AAGACCCCGACAGCAGATGATTCAGATGACAGCCGAGAAAAGACTGGCATAGAAGAGAAAGGAGAACTGA CCGACGCCTATCAGCTGCAGGTAGCTGAAGAAATGGCAAAGGAGATCAAGAAGAAAATAAGAAAGAAGCT AAAGGAACAGCTGACCTACTTTCCTCCAGACACTCTATTGCATGACGACAAGCTGGCCAGTGAAAAAAGA AAGAAGAAGAAGAAGAAAGTGCCAGTGCCCACTAAGCCTGAGTCAAGTCCCTCAGATGTCTGTGACAGTG CAGTTGAAGGGGAACAAAAGAAAGAAGGTACCCCTGAGGACTCTCAGCACATGGAGGGAATCTGCTCGAG AGAGCAGGATGTGGATGCCACTGTGCCAGAGAACGCAAAGCCCAAACCCAAGAAGACAAAGAAGAAGACT AAAGCAGTTTCAAATGATAATGAAGACACTAATGGAGATGGTGTTCATGAGATAACAAGCCGAGACAGCC CAGTTCATCCCAAGTGCTTGCTGGATGATGACCTCGTCATGGGAGTCTACATTCACCGAACCGATAGACT TAAATCTGACTTTATGATTTCTCACCCAATGGTAAAGATCCATGTGGTTGATGAGCACACTGGCCAGTAC GTCAAGAAAGATGACAGTGAACGTCCTGTTTCATCTTATTATGAGAAAGACAACGTGGACTATATTCTCC CTATTATGACACAGCCATATGACTTTAAAAAGCTAAAGTCAAGGCTTCCGGAGTGGGAGGAGCAAGTTAT TTTTAATGAAAATTTTCCCTATTTGCTTCGAGAGTTTGAAGAATGTCCAAAAGTCATCCTGTTCTTTGAG ATTCTTGACTTTTTAAGCATGGATGAAATCAAGAATAACTCTGAGGTTCAAAACCAAGAGTGTGGCTTTC GGAAAATTGCCTGGGCGTTTCTTAAGCTTCTGGGAGCCAATGGGAATGCAAACATCAACTCAAAACTTCG CCTGCAGCTCTACTACCCACCGACTAAGCCTCGATCCCAGCTAAATGTCGTCGAGGTTTTTGAATGGTGG TCCAAGTGTCCAAGAAATCGTTATCCATCAACATTGTATGTAACCGTACGAGGATTGAAAGTCCCGGACT GTATAAAGCCATCTTACCGCTCTATGATGGCTCTCCAGGAGGAAAGGGGTACACCAGTGTACTGTGAACG TCACCGTGAAACAAGTTCCGTGGACACAGAACCTGGACTAGAAGATTCAAAGGAGGAAGTGAAGTGGAAA CGTCTGCCTGGCCAGGCCTGTCGTATCCCAAACAAGCATCTCTTCTCACTGAATGCTGGAGAACGCGGCT GTTTCTGTCTTGATTTCTCTCACAATGGAAGGATATTAGCAGCAGCCTGTGCCAGCCGAGACGGATATCC GATTATATTATATGAAATTCCTTCTGGACGTTTTATGAGAGAATTGTGTGGCCACCTCAATATCATTTAT GATCTCGACTGGTCAAAAGACGATCGCTATCTCGTTACTTCATCCTCTGACGGCACTGCCAGAGTTTGGA AGAATGAAATCAATAGCACGAGCACGTTCAGAGTCTTACCTCACCCCTCCTTCGTCTACACGGCTAAATT CCACCCAGCCACACGGGAGCTGGTGGTTACAGGATGCTACGACTCTATGATAAGGATTTGGAAAATTGAT GCAAGGGAGGATGCTGCCATATTAGTGCGCCAGTTGGATGTGCACAAGAGCTTTGTCAACTCCATCTGTT TTGACGATGAAGGTCACCACATGTACTCAGGAGACTGCATCGGGGTCATCGTTGTCTGGGACACGTACGT CAAAGTGAATGATGTGCAACACTCCGTGCGCCACTGGACGATAAATAAGGAAATTAAGGAAACTGAATTC AGGGGAGTTCCAATAAGTTACCTGGAGGTTCATCCCAATGGAAAGCGTTTGCTAATCCACACCAAAGACA GTACTCTGAGGATTATGGACCTGCGGATATTGGCAGCCAGGAAATTTGTGGGTGCAGCAAATTACCGTGA GAAGATCCATAGCACCTTGACGCCGTGCGGGACTCTGCTCTTTTCTGGGAGTGAGGATGGGATAGTATAC GTTTGGAACCCAGAGACAGGAGAACAAGTGGCAATGTACTCTGACCTGCCATTCAAGTCCACAATCCGAG ACATCTCTTACCACCCCCTGGAGAATATGGTTGCCTTCTGTGCCTTTGGACAGAGCGAGCCGATTCTTCT TTATATTTATGATTTCCAGGTAGCCCAGCAGGAGGCTGAAATGTTGAAACGCTATAGTGGGACACTCCCG TTGCCTGGGATACATCAGAGTGAGGATGCTTTATGCACCTGCCCTAAGCTGCCCCAGCAGGGCTCTTTTC AGATTGATGAATTTGTCAACACTGAAAATAGTTCATCAAGAAAGATTCAACTTGTGAAACAGAGACTCGA AACTGTCACAGAGGTGATACGATCCTGTGCTGCAAAAGTCAACAAAAATCTCTCAATGACTTCGCCACCA CCAGGCCCTGCAAAGAAGCCCAGGGTAAAGCAGTCGTTCGTGCTGACCACTGATGAGATCATACATCAGT TTGGACTCCCCCAGACTGCATTTATCAGCATAGAGAGAGGGCCTTTTGTGCGCCATGTTGATCCACCACC AATGGTAGTGGCTCTTTATGACTACACAGCCAGCCGATCAGATGAACTAACCATCCATCGTGGAGACATT ATCCGAGTGTATTTCAAAGATAATGAAGACTGGTGGTACGGCAGCGTAAGAAAGGGGCAGGAAGGGTTCT TTCCAGCTAATCATGTGGCCAGTGAAACACTGTATCGAGACTCCCCTCCGAAGGTAAAGGAGCGCTCCCC TCCTTTAACTCCCAAGGAGAAAACTAAACCAGAAAAGCCTCTGGCTTCTCAAAAGGGTGGCCATGAAGAG GAGACGAAGTCACAAACCAACTGAGAGGTGTGTGTGGAAAGGAGGGAAGGAAGGGCCGGCTGCCATGCTC CTTGGCCTCGCTGATGGTGGGGGGGGCACTTGTGGGCTGATTGCGGTCCAGCCGTGGGGGCTTTTGTTTA AGATAAAATAAAACAGCTGACAGAGACAACTCCTTCTTCCCCCTCCTACTGCCTCCATGAGCACTTCCCA TATGGCTGCTGCTGCTGAGACCAAGGAATGACAGTCGCTCAGTAAGGGCAGACCCCTGGATCCCAGACTG GGCCCGCAGCCTGTGGGGCATTCTGAGAAGGGCAAAGATCAAAACGTGGAGGACCGAGGACACAAAGTAG ATATGGAGACAAAGAAAAGCGAGCCGGTGGTCCGCAAAGTCACCCTGATAGAGTAAAAAGCTGGAGACGA AGCAGAAGCCGCCTGATGAGTGCACATGATGTGACGTCCACGCCAGACGGAGTTCTCGTTAGATTTTGGG TGAAAAGAAGCAGTATTTATTGACCTTAAAAATCTGAAGCAAACTTAGCTCTAAAAAGAAAAAAATCACT GTGGCCTTTGGGCTTAATTGTTATGAACCATTGTGAGTGTTACTGGTCAAAGTATTGTGCTTGTGCTATT AGTAACTACATCAGAATTATATTGCTGCTGCTGGAAAAGCCAATAGAGAAAACCCCAGAACTGCTACTCA GTAAATGTGAATAACAGTGTGCGCAGTGTTAGTCATTCACTAAGCCATCATCCCGTGTACTTGTGGCCAT GATGTGGCTGTGCCGTAGGCCGTCATCCCGTGTACTTGTGGCCATGATGCAGATGCTCTGCTTTTGAAGT TAGCCGCGTGTAAAACCTAAGTAGCTGCTGGATATTGTGTCTTCAAACAGCCCCAGTAACAAACGCGGTA GAAACGACAGTTTGCTTTTGAGAACTAAATGTTTGTGTTTCCCTTGAGTGAATGTATGTCACTCAACACT CCTAGACTAAGAATGATTTTGGCTGGACTGACAACAGTACACCAAATTTATTTTTATTTTTTAGACAGAG TTTATATTTAACAGCATAAGTAATTTATGGATGTAAACTCTGTAGTGGTTATAGTTTAGTCTGAATATTT GTTTTTAAATTATTACACAGCATTATTTATGTCTTTAGTTTCAGAATAACAGTGCCATTACCATATGTTT GTATGTTGCAGTTGGATATAGCTGAGACTCCTCCATCTTGGTTTTCTCTTCACTCATCCATTTCTTCAGC TGCATGATAAGTCTGTCAGTAAAGCATTTAACCCCAACAAGGTCAGACTATGGTAAAGTTAGAAAAACAT ATTTACCGAAGTACACAATTGATCTCATTGAAATAAGAAGTGAGAACCGCTCTGTTGAGCACTGAGGTGT AAACGTGCCCAGATGCACAGCGTGAGTAGCTTCGAGTACGCATCTGTTTGCACATGTTGTAGAAGCTTAA AATGGGAACATGACCCTGCTGTGGAGAACCTTGGTAGAATTGTTGTTAGTACTAAAGGAGATTTCCAGCG CATACAGGGTGGCACTGAAAACTGCTGAAGCCCACAGCCCTCAGCCCCGAAGGCTCAGCGGCCAGTACCA GCGGCCTTGCTGTGGTTGTGTGTCTGCGTCCCTGGCACACTTGGGCAGGCAGCTCAGTCACTCTGAACTT GCTTCTCTTCTGTCTTGTGAGTGTGCTGCTTGAGACAATGAAAAATAAAGCTGTGTTCCTACCCCTTCAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6467369 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6467369 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005590 same experiment
	EMAGE:29919 same embryo
	EMAGE:29782 same embryo
	EMAGE:29781 same embryo
	EMAGE:29942 same embryo
	EMAGE:29798 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source