EMAGE mouse gene expression spatial database

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EMAGE:30923

Gcat glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) ( MGI:1349389)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005596_01	euxassay_005596_02	euxassay_005596_03	euxassay_005596_04	euxassay_005596_05

euxassay_005596_06	euxassay_005596_07	euxassay_005596_08	euxassay_005596_09	euxassay_005596_10

euxassay_005596_11	euxassay_005596_12	euxassay_005596_13	euxassay_005596_14	euxassay_005596_15

euxassay_005596_16	euxassay_005596_17	euxassay_005596_18	euxassay_005596_19

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

reproductive system

moderate

regional

moderate expression: see section 08

liver right lobe

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 weak expression: see section 17 18 19

stomach

moderate

regional

moderate expression: see section 03 04 weak expression: see section 05 06 07 08

midgut

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18

esophagus

moderate

regional

moderate expression: see section 07 08 09

pancreas

moderate

regional

moderate expression: see section 07 08 09 10 11 12

submandibular gland primordium

strong

regional

strong expression: see section 13 moderate expression: see section 04 05 06 11 12

thymus primordium

strong

regional

strong expression: see section 07 moderate expression: see section 06 08 09 10

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 16 17 18

epidermis

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 01 02 03

naris

moderate

regional

moderate expression: see section 09 10 11 12 13

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

lower jaw incisor

moderate

regional

moderate expression: see section 08 09 10 11 12

upper jaw incisor

moderate

regional

moderate expression: see section 09 10 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4935
Entity Detected:	Gcat, glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) ( MGI:1349389)
Sequence:	sense strand is shown >T4935 GTCACGTGACGGGGAGCACTAGACTTCAGGCTGGCCAGCGCACTTTAGCCTGTGGGCGCGATGTGGGCCA GCTTCATGTGGCACGGGGCGCTGTCCCCTGGCCGCCGCGCACATTCAGCGCTGGCTCAGTTGCGCTGCAT CCTGGACAGCGAACTGGAAGGGATCCGCGGAGCCGGCACCTGGAAGAGTGAGCGTGTGATCACGTCCCGC CAGGGACCGAGCATCCGCGTGGACGGCATCTCGGGAGGAATCCTCAACTTCTGTGCCAATAACTACCTGG GCCTGAGCAGCCACCCTGCAGTGATCCAGGCAGGTCTGCAGACTCTGGAGGAGTTTGGAGCTGGACTCAG TTCTACTCGATTTATCTGTGGGACCCAGAGCATCCATAAGAATCTAGAAGCCAAGATAGCCCACTTCCAC CAGCGTGAGGACGCCATCCTCTATCCCAGCTGTTTCGATGCCAACGCTGGCCTATTTGAGGCTTTGCTGA CCCCAGAGGATGCGGTTCTGTCAGATGAGCTGAACCATGCTTCCATCATTGACGGCATCCGACTGTGCAA GGCCCACAAGTACCGTTACCGCCACCTGGACATGGCTGACCTGGAAGCCAAGCTGAAGGAGGCTCAGAAG CACAGGCTGCGCCTGGTGGCCACAGATGGAGCTTTCTCCATGGATGGTGACATCGCTCCCCTACAGGACA TCTGCCGCCTTGCCGCCCAATATGGTGCCCTGGTCTTTGTGGATGAATGCCATGCCACTGGCTTTCTTGG GCCCACAGGACGGGGCACAGATGAGCTGCTAGGTGTCATGGACCAGGTCACCATCATCAACTCCACCCTG GGGAAGGCGCTGGGTGGAGCATCAGGGGGATATACCACCGGGCCTGAGCCACTGGTGTCCCTGCTACGGC AGCGTTCTCGGCCCTACCTCTTCTCCAACAGCCTGCCCCCTGCTGTAGTCGGCTGTGCTTCTAAGGCCCT GGACCTGCTCATGGAGAGCAATGCCATCATCCAGTCTATGGCTGCCAAGACCCGGCGGTTCCGCAGTAAG ATGGAAGCTGCTGGTTTTACTGTCTCAGGAGCCGATCACCCCATCTGCCCTGTGATGCTGGGAGATGCAC GGTTGTCTTCTCAAATGGCAGATGACATGTTAAAGAAAGGTATCTTTGTCATCGGATTTAGCTACCCTGT GGTCCCTAAGGGCAAGGCCCGGATCCGGGTGCAGATCTCAGCTGTACACAGTGAGGAAGACATTGACCGC TGTGTGGAAGCCTTCGTGGAGGTGGGGCGGCTGCATGGAGCTCTGCCCTAAGCTCTTGGTAGGGACCTGC AGAGCCAAAGTTCCTCCACTGCCGCTGACATAGGGCTCCTCTGGTCAGCCCAGATCAGAGGCTGTGACTC AACCCAGACTTCTAGAGCCTGGTTGAAAATGGGCCTGTACTTGTCATCAGAGAGACAACAATGTGGAATT TGGCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5125361 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5125361 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005596 same experiment
	EMAGE:29251 same embryo
	EMAGE:29256 same embryo
	EMAGE:29796 same embryo
	EMAGE:31963 same embryo
	EMAGE:31962 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source