EMAGE mouse gene expression spatial database

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EMAGE:30944

Iars2 ( MGI:1919586)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005121_01	euxassay_005121_02	euxassay_005121_03	euxassay_005121_04	euxassay_005121_05

euxassay_005121_06	euxassay_005121_07	euxassay_005121_08	euxassay_005121_09	euxassay_005121_10

euxassay_005121_11	euxassay_005121_12	euxassay_005121_13	euxassay_005121_14	euxassay_005121_15

euxassay_005121_16	euxassay_005121_17	euxassay_005121_18	euxassay_005121_19	euxassay_005121_20

euxassay_005121_21	euxassay_005121_22	euxassay_005121_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8585
Entity Detected:	Iars2, ( MGI:1919586)
Sequence:	sense strand is shown >T8585 CTCGCCGCCGAGCTCTTCAAGGGTCTCTACCTTTAGGTACCGGGCGGCTGCCGTGGGCCGTGTGCGGAGG GCTCCGCCCGGCTCGTCCACCATGCACTGGGGGCTGTGCCCTCGCGGGCCCGGGGCGGCGGCGGTCGCCG CGGCTGGGAGCTTCTGGGGGCCAGCCCGTTTGCCCAGCCGCCTAGGGTGTCTGGGGATGACGAGGAGGCT CGTGGTACGATCCGTGGCCGGGGCGGACAGCCCCCAGAGCAGCTCGAAAGGCGGCAGATACCGTGACACG GTGCTGCTGCCGCAGACCAGCTTCCCGATGAAGCTGCTGGGCCGCCAGCAGTCCGACATGGAGCTGGAGA TCCAGCAGAAATGTGGCTTTTCAGAGCTTTATTCCTGGCAAAGAGAAAGAAAAGTAAAGACGGAATTTTG CCTTCATGACGGGCCTCCTTATGCCAACGGTGACCCTCATGTTGGGCATGCCCTGAACAAGATTTTGAAA GACATTGCCAATCGATTCCATATGATGAGAGGTTCCAAGGTACACTTTGTGCCTGGCTGGGATTGCCATG GCTTGCCCATTGAAACAAAAGTGTTATCAGAGCTCGGTGTAGATGCCCAGAGTCTTTCAGCCATGGAGAT CAGGGAGAAAGCCAGATCATTTGCTCAGGCAGCCATTGAAAAGCAGAAATCGGCGTTTGTCCGCTGGGGA GTGATGGCAGACTGGAATAACTGCTATTACACATTTGACCCAAAGTACGAAGCCAAGCAGCTGAGAGTAT TCTACCAAATGTATGAGAAGGGCTTGGTTTATCGATCTTACAAACCAGTATATTGGTCTCCCTCATCAAG GACTGCGTTGGCTGAAGCAGAGCTTGAATACAACCCTGAGCATGTCAGTCGGTCGATATACGTAAGATTC CCCCTCCTGAGACCTCCTCCGAAGTTGGAGTCTCTTACAGATGCCTCATCCCCTGTTAGCGTTTTAGTTT GGACCACCCAGCCTTGGACAATTCCCGCCAACCAGGCAATTTGCTATATGCCAGAGGCAAAGTATGCTGT GGTGAAATGTTCTGCCTCTGGGCACCTCTACATCCTGGCTGAAGATAAAATAGCACCTGTCGCTTCTGCC TTGGAAACAACATTTGATGTTGTTGCAGCATTTTCAGGTGTGGATCTGGAAGGTGGCACTTGTAGTCACC CTTTAACTCCCGATAAAGTGTCTCCTCTTTTACCTGCTACCCACGTGACTATGGCCAAAGGAACAGGGTT GGTTCACACAGCCCCAGCTCACGGTATGGAAGATTACAGCGTAGCATCTCAGCACAGCCTCCCTATGGAC TGTTTAGTGGATGAAGGCGGAATGTTCACCGATGCCGCAGGCCCTGAACTTCAGAACAAGGCTGTACTTA AAGAAGGAACAGATGTGGTTATAAAGATGCTTCAAGCTACCAAGAATGTGTTGAAAGAAGAGAACATTGT GCACAGCTATCCCTGTGACTGGAGAACTAAGACACCCGTGCTGATTCGAGCCAGCAAGCAGTGGTTTGTA AATATCACGGACATCAAGGCTGCAGCTAAGGAGTCGTTGAAGACGGTGAAGTTCATTCCCGGAGCCGCAC TGAACAGCATGACGGACATGCTGGACAGACGGCCGTACTGGTGCATATCCCGGCAGAGAGTCTGGGGGGT TCCCATTCCTGTGTTTCATCATAAGACAAAAGATGAATATTTGATCAACAGCCAAACCACTGAGCATATT ATTAAACTCGTCGAGCAGCACGGCAGTGATGTGTGGTGGACCCTCCCTGCTGAGCAGCTTCTCCCGGCAG AAGTCCTAGCTCAGGCTGGTGGCCCTGGGGCTTTGGAATACGCGCCAGGGCAGGACATTTTGGACATCTG GTTTGACAGTGGAACTTCATGGTCTTGTGTTCTTCAAGACACGCAGCAGAGGGCAGACTTGTACCTGGAG GGAAAGGACCAGCTCGGGGGCTGGTTCCAGTCTTCCTTACTAACAAGTGTGGCCACCAGGTCAAAAGCAC CTTTCAGGACAGTGATGGTTCATGGATTTACACTTGGAGAAAAAGGAGAAAAGATGTCCAAGTCCCTCGG GAATGTCATTAATCCTGACACTATCATCAGTGGAGGAAAAGACCACAGCAAAGAACCTCCCTACGGAGCT GACATCCTGCGCTGGTGGATAGCTGAGTCCAATGTCTTCACGGAGGTGACCATTGGCCCCTCTGTACTCT CTGCTGCCAGAGATGACATCAGCAAGCTCAGAAACACACTCCGCTTTCTCTTGGGAAATTTGACCGGTTT TAACCCCGAGACAGATTCTGTTCCCGTGAAGAATATGTATGTCATAGACCAGTATATGCTGCACTTAATT CAGGATTTTGCGACAAAGATTACTGACTCATACAAACAGTATGACTTTGGGAAAGTTGTCAGGCTGTTGA AAGCCTTCTACACCAGAGAACTGTCCAGCTTTTACTTCAGCATAGTCAAGGATAGGCTCTACTGCGAAAA CGAGAAGGATCCCAAGCGCCGCTCGTGTCAGACAGCATTGGCAGAAATTTTGGATGTGTTGGTCCGTGCT TTTGCCCCCATTCTTCCTCACTTGGCAGAAGAGGTGTTTCAGCACATTCCCTATGTTACAGAACCCAAGA GTGTTTTTCGCACTGGCTGGATTAATACAAGCTCTATTTGGAAGAAGCCAGGACTGGAGGAGGCAGTGGA AAGTGCGTGCGCCATGAGGGACTCCTTTCTTGGCAGCATCCCTGGCAAAAATGCTGCTGAGTACGAGGTC ATCATAGTGATCGAGCCAGGGCTGCTGTTTGAGATCATGGAGATGCTCCAAGCTGAGGAGACCTCTAGCA CGTCTCAGCTGAATGAGCTGATGATGGCTTCCCAGACGACATTGCTGGCTCAGGAGCCACGGGAAAGAAC TGCAGGTGACATTGAGCTCACGGGGACATTCGTCATCAATCTAGAAGGTGGTGACATTCGTGAGGAGTCT TCATATAAAGTCATTGTGGTACCGACTGCCAGAGAGAAGTGCCCTCGATGTTGGAAGCATACCTCTGAGA CTGCAGACGCTCTCTGTCCTCGGTGTGCAGAGGTCATTGGTGCAAAGTAGCAACCTGATGTCAACAGACG ACGACACTCCCCACAGTACTGACTGGCTGAGGAGATGAGCTGTTAGACAGTTCCCTAGAAAGAAATCCAA GGCTTACACAGAAAGTAGAGGGGCAGATAGATGGTAGCAGATGGGGTAAGAATCAGAAGATTAAAAGAAT ATTTCTTGTAACTATAGAAAGAATAATGTATGTATATATATATATATGGAGATATATATGCAGATATACA TATATACAGGGCACACACACACACACACTCATGACTTCTTCAGAGACAGACACTGAATATGGTTACTTTC AATGTGGCAGAATGTTTTATATTTTATTAAAACTTTTTTGACTCAGTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5715723 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5715723 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005121 same experiment
	EMAGE:29482 same embryo
	EMAGE:30916 same embryo
	EMAGE:29485 same embryo
	EMAGE:29577 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source