EMAGE mouse gene expression spatial database

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EMAGE:30976

Tfap2a transcription factor AP-2, alpha ( MGI:104671)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004935_01	euxassay_004935_02	euxassay_004935_03	euxassay_004935_04	euxassay_004935_05

euxassay_004935_06	euxassay_004935_07	euxassay_004935_08	euxassay_004935_09	euxassay_004935_10

euxassay_004935_11	euxassay_004935_12	euxassay_004935_13	euxassay_004935_14	euxassay_004935_15

euxassay_004935_16	euxassay_004935_17	euxassay_004935_18	euxassay_004935_19	euxassay_004935_20

euxassay_004935_21	euxassay_004935_22	euxassay_004935_23	euxassay_004935_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

oral epithelium

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

metencephalon lateral wall

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21

metanephros

strong

spotted

strong expression: see section 05 06 07 08 09 14 15 16 17

medulla oblongata lateral wall

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 16 17 18

lower jaw molar

strong

regional

strong expression: see section 06 18

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 19 20 21 22 23 24

upper jaw molar

strong

regional

strong expression: see section 06 18

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 10 11 13 14 15

vibrissa

strong

regional

strong expression: see section 02 03 04 05 06 07 17 18 19 20 21 22

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 15 moderate expression: see section 09 16 17

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22

naris

strong

regional

strong expression: see section 09 14 15

hand

strong

regional

strong expression: see section 01 02 03 04 05 06 24

midbrain mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

nasal septum

moderate

regional

moderate expression: see section 12

spinal cord mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14

tongue epithelium

strong

regional

strong expression: see section 10 11 12 13 14

leg

strong

regional

strong expression: see section 22 23

urethra of male

strong

regional

strong expression: see section 11 12 13

retina

moderate

regional

moderate expression: see section 01 02 03 22 23 24

conjunctival sac

moderate

regional

moderate expression: see section 01 02 03 04 05 20 21 22 23 24

foot

strong

regional

strong expression: see section 05 06 07 08 22 23 24

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 15 16 17 18

naso-lacrimal duct

strong

regional

strong expression: see section 07 08 18

physiological umbilical hernia epidermis

strong

regional

strong expression: see section 12 13

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 09 10 11 12 14 15 16

lower jaw incisor

strong

regional

strong expression: see section 09 10 11 13 14 15

upper jaw incisor

strong

regional

strong expression: see section 09 10 11 13 14 15

glans of male genital tubercle

strong

regional

strong expression: see section 11 12 13 14 15

pharynx

strong

regional

strong expression: see section 10 11 12 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6939
Entity Detected:	Tfap2a, transcription factor AP-2, alpha ( MGI:104671)
Sequence:	sense strand is shown >T6939 GCCGATTTGCACAGGAAGAGTGGTTCCTAATGTTTTATTTTGATGCATGTACGACACCGCATGGAGAGTC GCAGGAGCTCATGGGTCTGCGGTTTGGCTCACTCCAGAAGGGGTTGTGCATCCCCATCCGAATTCTGTGT TGTTGTCCACGTTGCACCTGGGAAACCTTACAGTCCAAACGCCCGGATGAATAGTCAGTCCGATTCAGCT TCATACAAACCGGAGGAAAATCATTTAACCCCCGGATCTGTCGAGTAACCTCTTCAGGCCCGCACTTTGC GCTAACCCAGAGAGTAGCTCCGCTGGGGTGCGAGGCGCAGAGGGGCAAATCCGATCACGCCGATCCATGA AAATGCTTTGGAAACTGACGGATAATATCAAGTACGAAGACTGCGAGGACCGTCACGACGGCACCAGCAA CGGGACGGCAAGGTTGCCCCAGCTGGGCACTGTAGGTCAATCTCCCTACACCAGCGCCCCGCCGCTGTCC CACACCCCTAATGCCGACTTCCAGCCTCCCTACTTCCCCCCGCCCTACCAGCCTATCTACCCCCAGTCGC AAGATCCTTACTCCCACGTCAACGACCCCTACAGCCTGAATCCTCTGCACGCCCAGCCGCAGCCGCAGCA CCCGGGCTGGCCCGGCCAGAGGCAGAGCCAGGAATCTGGGCTCTTACACACACACCGGGGCTTACCCCAC CAACTGTCGGGCCTGGACCCTCGCAGGGACTATCGGCGGCACGAGGACCTCTTGCACGGCCCGCACGGGC TCGGCTCTGGACTCGGGGACCTCCCGATCCACTCCTTACCTCACGCCATCGAGGACGTCCCGCACGTAGA AGACCCGGGTATTAACATCCCAGATCAAACTGTAATTAAGAAAGGCCCCGTGTCCCTGTCCAAGTCCAAC AGCAATGCTGTCTCCGCCATCCCTATTAACAAGGACAACCTCTTCGGTGGCGTGGTGAACCCCAACGAAG TCTTCTGTTCAGTTCCGGGTCGCCTGTCGCTCCTCAGCTCCACCTCGAAGTACAAGGTCACGGTGGCGGA AGTACAGCGGCGCCTCTCACCGCCCGAGTGTCTTAACGCGTCGCTCCTGGGCGGAGTACTGCGGAGAGCG AAGTCTAAGAATGGAGGGAGATCTTTAAGAGAAAAACTGGACAAGATAGGATTGAATCTGCCAGCAGGGA GACGTAAAGCTGCCAACGTTACCCTCCTCACGTCACTAGTGGAAGGAGAAGCCGTCCACCTAGCCAGGGA CTTTGGGTACGTGTGCGAAACTGAATTTCCTGCCAAAGCAGTAGCAGAATTTCTCAACCGACAACATTCC GATCCCAATGAGCAAGTGGCAAGAAAAAACATGCTCCTGGCCACAAAACAGATCTGCAAAGAGTTCACTG ACCTGCTGGCTCAGGACCGATCTCCCCTGGGGAACTCGCGGCCCAATCCTATCCTGGAGCCTGGCATCCA GAGTTGCTTGACCCACTTCAACCTCATCTCCCATGGCTTCGGCAGCCCGGCGGTGTGTGCAGCGGTTACG GCCCTGCAGAACTATCTCACCGAGGCCCTCAAGGCCATGGACAAAATGTACCTCAGCAACAACCCCAACA GCCACACGGACAACAGCGCCAAAAGCAGTGACAAAGAAGAGAAACACAGAAAGTGAGGCTCTCCTCCCGC CCCGCCCCCGCCCGCCTCACCGGCCCGCGTCCCCCCACTCGCCCCTCCCCCACCCGGCAGGTGACAGCTC CGGGACCAGCCACTCCTACTGCTGCTGCTACTCTGCGTTGCCGCGCCGCCCTTCGTCCCCGGAGTCCTCC GGATTGCTCTCTCGACTGCCAGTGGGGCAGCCGCTGCCTACTCAGCGCCTTGCCTCAACTTCCCCATCAG CACCAACACCCCCTTTACCCTCGTGTGGAGCCTAAGAGAACAGAACAGGTCGTGAAGCCAGCAAAGAAAA GTTCTGTCGCGTTTGTGAACCTTTTTTTTTAAAATCAAATCCACAACAAACATTAAAACTTTTTTTTTAA AAAGGACGTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3983850 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3983850 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004935 same experiment
	EMAGE:30184 same embryo
	EMAGE:30685 same embryo
	EMAGE:30079 same embryo
	EMAGE:30077 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source