EMAGE mouse gene expression spatial database

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EMAGE:31026

Hcls1 hematopoietic cell specific Lyn substrate 1 ( MGI:104568)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006086_01	euxassay_006086_02	euxassay_006086_03	euxassay_006086_04	euxassay_006086_05

euxassay_006086_06	euxassay_006086_07	euxassay_006086_08	euxassay_006086_09	euxassay_006086_10

euxassay_006086_11	euxassay_006086_12	euxassay_006086_13	euxassay_006086_14	euxassay_006086_15

euxassay_006086_16	euxassay_006086_17	euxassay_006086_18	euxassay_006086_19	euxassay_006086_20

euxassay_006086_21	euxassay_006086_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 07 08 09 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6570
Entity Detected:	Hcls1, hematopoietic cell specific Lyn substrate 1 ( MGI:104568)
Sequence:	sense strand is shown >T6570 GGCAGCCGCTGGAGGGGGCGCCTGGTGTAGATGTGAAAAGCCGTAACCAGGAACCAGTAAAGATGTGGAA GTCTGTAGTGGGGCATGATGTATCGGTTTCCGTGGAGACCCAGGGTGATGACTGGGATACAGACCCTGAC TTTGTGAATGACATCTCCGAGAAGGAGCAACGGTGGGGAGCCAAGACCATTGAGGGCTCTGGACGCACAG AGCACATCAACATCCACCAGCTGAGGAACAAAGTGTCAGAGGAGCACGACATCCTCAAGAAGAAGGAGCT GGAATCGGGGCCTAAGGCATCCCATGGCTATGGCGGTCGGTTTGGAGTGGAGAGAGACCGGATGGACAAG AGTGCCGTGGGCCACGAGTATGTTGCTGATGTGGAGAAACACTCATCTCAGACTGATGCCGCCAGAGGCT TTGGGGGCAAATATGGAGTTGAGAGGGACCGGGCAGACAAGTCAGCGGTGGGCTTTGACTACAAAGGAGA AGTGGAAAAGCATGCATCTCAGAAAGATTACTCTCATGGCTTTGGTGGCCGCTACGGGGTAGAGAAGGAT AAACGGGACAAAGCAGCCCTGGGATACGACTACAAAGGAGAGACGGAGAAGCACGAGTCTCAGAGAGATT ATGCCAAGGGCTTTGGTGGCCAATATGGAATCCAGAAAGACCGAGTGGATAAGAGTGCTGTTGGCTTCAA TGAAATGGAGGCCCCAACCACGGCGTATAAGAAGACAACACCCATAGAAGCTGCTTCCAGTGGTGCCCGT GGGCTGAAGGCAAAATTTGAGTCCCTGGCTGAGGAGAAGAGGAAGCGAGAGGAAGAAGAGAAGGCACAGC AGATGGCCAGGCAGCAACAGGAGCGAAAGGCTGTGGTAAAGATGAGCCGAGAAGTCCAGCAGCCATCCAT GCCTGTGGAAGAGCCAGCGGCACCAGCCCAGTTGCCCAAGAAGATCTCCTCAGAGGTCTGGCCTCCAGCA GAGAGTCACCTACCGCCAGAGTCTCAGCCAGTGAGAAGCAGAAGGGAATACCCTGTGCCCTCTCTGCCCA CGAGGCAGTCTCCATTGCAGAATCACTTGGAGGACAACGAGGAGCCCCCAGCTCTGCCCCCTAGGACCCC AGAAGGCCTCCAGGTGGTGGAAGAGCCAGTGTACGAAGCAGCACCCGAGCTGGAGCCGGAGCCAGAGCCT GACTATGAGCCAGAGCCAGAGACAGAGCCTGACTATGAGGATGTTGGGGAGTTAGATCGGCAGGATGAGG ATGCAGAGGGAGACTATGAGGATGTGCTGGAGCCCGAGGACACCCCTTCTCTGTCCTACCAAGCTGGACC CTCAGCTGGGGCTGGTGGTGCGGGGATCTCTGCTATAGCCCTGTATGATTACCAAGGAGAGGGAAGCGAT GAGCTTTCCTTTGATCCAGATGACATCATCACTGACATTGAGATGGTGGATGAAGGCTGGTGGCGGGGCC AATGCCGTGGCCACTTTGGACTTTTCCCTGCAAACTATGTCAAGCTCCTCTAATGACCAGCCCATTGTCT TCCGACTTCCCGAATTCGAAGCTGCTCTGCCTCCCTCTTCCCACTCCATGGTACTGCTGCAAGGACCTGG CTGAACATCATGAGATGCCTGAAGTTCTGGCAGTCTGTCTCCCGCCTCTTTAAGAGCTTTAGGTAGAATC GCTCCAGGTGGGGGTGGGGGTGGGGGTGGGATCCCTCTGTCCCTCTGTGACCACTCTTCCCTGAGGTAGC TCATGAAATCATCTTGCAGACCTGCCTCCTTCAGCCGCACCCCAGCTCTGCCAACCTTGCTCTAGAGTGC TGGGATTCCCTTGCCCCGACCCTGGGTGCCAGCCTAGAGGGGAGGCTCTCACAGGGCTGCCTGATTCGCC CTGTTGTGCTTTTGCTCATTTTTCTTCCCTTAGCAGACAAATTGGAACTGCCCTTCTGTTTAGTCCTAAA ACTGAAAATAAAATGAGACTGTGGCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3593456 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3593456 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006086 same experiment
	EMAGE:31606 same embryo
	EMAGE:30901 same embryo
	EMAGE:31615 same embryo
	EMAGE:31184 same embryo
	EMAGE:30216 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source