EMAGE mouse gene expression spatial database

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EMAGE:31059

C530008M07Rik ( MGI:2685362)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_010345_01	euxassay_010345_02	euxassay_010345_03	euxassay_010345_04	euxassay_010345_05

euxassay_010345_06	euxassay_010345_07	euxassay_010345_08	euxassay_010345_09	euxassay_010345_10

euxassay_010345_11	euxassay_010345_12	euxassay_010345_13	euxassay_010345_14	euxassay_010345_15

euxassay_010345_16	euxassay_010345_17	euxassay_010345_18	euxassay_010345_19	euxassay_010345_20

euxassay_010345_21	euxassay_010345_22	euxassay_010345_23	euxassay_010345_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

skin

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

weak

regional

weak expression: see section 05 06 07 08 09 10 23 24

stomach

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10

midgut

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37610
Entity Detected:	C530008M07Rik, ( MGI:2685362)
Sequence:	sense strand is shown >T37610 GGGTGACAGATTTGAAGGCTACGAAAACCCTGAGGCCGTGAAAGCCAATGCACTCTATACTCACTCGAGG CTCCGCTCCTCGTTTGTGTTTAAACCAACTTTACCGGAACAAAAAGAAGTCAACAGCTGTACAACTGGCT CTTCAAATTCCACCATCATCGGTTCTCAAGGAAGCGATACACCTAATGAGGTCCCAGATACCTCCACAAA TGCACCGCCCTTGACAGAAAAACCTTTGCCTGAACCAAGCTCCAAGCTCCCAACACAACAAGAGGAACCA AAAATGCACAACTTGCAGGTTCCCGAAAAGCAACCAGAAGCCTTAAACCAAAGAACAAATGGCCGAGCAG AATTAAACAACTGTATCTATACAAACCTGTGTGTCAATAAGCAGAGGGAGAACACAGAAAATCAGCAAAA TGACAATCTGCTCAAAAGGCGCAGTTTCCCGTCCTTTGACCACTCGAAGGTCAATTTAGAGCATGGGAAT AGCAAGAATTATGTTTATAGTACCCTTACCAGAAATCGGATTCGACAACCAGAAAAACCCAAGGAGGTTG TGCTGAAGAGCTCTAAAAGCATGCACAACGTGACCCACAGCGCAGAGGAAGATGATGACGAGGTTATCGA GAGAGACCCTCCAAGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 82061. Forward Primer - name:082061_F_cDNA_C530008M07Rik, sequence:GGGTGACAGATTTGAAGGCTAC; Reverse Primer - name:082061_N_SP6_cDNA_C530008M07Rik, sequence:ACTTGGAGGGTCTCTCTCGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_010345 same experiment
	EMAGE:30119 same embryo
	EMAGE:31022 same embryo
	EMAGE:31021 same embryo
	EMAGE:30146 same embryo
	EMAGE:30145 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source