EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31060

Olfr877 ( MGI:3030711)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_013858_01	euxassay_013858_02	euxassay_013858_03	euxassay_013858_04	euxassay_013858_05

euxassay_013858_06	euxassay_013858_07	euxassay_013858_08	euxassay_013858_09	euxassay_013858_10

euxassay_013858_11	euxassay_013858_12	euxassay_013858_13	euxassay_013858_14	euxassay_013858_15

euxassay_013858_16	euxassay_013858_17	euxassay_013858_18	euxassay_013858_19	euxassay_013858_20

euxassay_013858_21	euxassay_013858_22	euxassay_013858_23	euxassay_013858_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

heart ventricle

weak

spotted

weak expression: see section 08 09 10 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39138
Entity Detected:	Olfr877, ( MGI:3030711)
Sequence:	sense strand is shown >T39138 CCTGCATATCCCCATGTACTTTTTCCTCTTCAATTTGTCCTTCATAGATTTCAGTTACTCCACCACACTC ACCCCTAAAATGCTGGTGGGGTTTGTTCTGAGGAAGAACATCATCTCCTATGCAGGGTGTATGACACAGT TTTTCTTCTTCTGCTTCTTTGTCTTTTCTGAGTCTTACATCCTGTCAGCAATGGCATATGACCGCTATGT TGCCATCTGTAAACCACTGTTGTATAGTGTCACCATGTCTCCCCAAGTGTGTTCATACCTTTTGTCAGGT GTCTATGGGATGGGAGTTTTTGGGGCTGTGGCCCATATGGGAAACCTACAGTTTATAAGCTTCTGTGCTG ACAACATCATTAACCACTATATGTGTGACATAATTCCCCTCCTTGAGCTGTCCTGCAATAGCTCATACAT CAATTTGTTGGTGGTTTTTATTGTTGTGACCATTGGCATTGGAGTGCCCATTGTTACCATTTTTATATCT TATGGTTTCATCCTCTCTAGCATTCTCCACATTAGTTCTAAAGAAGGCAGGTCGAAAGCCTTTAGTACAT GTACGTCCCATATTATTGTAGTGTCTCTCTTTTTTGGGTCAGGAGCTTTTATGTACCTCAAACCACCTTC TAGTTTGCCTCTGGATCAGGGAAAAGTAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 152337. Forward Primer - name:152337_F_cDNA_Olfr877, sequence:CCTGCATATCCCCATGTACTTT; Reverse Primer - name:152337_N_SP6_cDNA_Olfr877, sequence:ATACTTTTCCCTGATCCAGAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_013858 same experiment
	EMAGE:32065 same embryo
	EMAGE:32067 same embryo
	EMAGE:30098 same embryo
	EMAGE:30088 same embryo
	EMAGE:31809 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source