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EMAGE:31119

Ysk4 ( MGI:1203481)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31119 EMAGE:31119 EMAGE:31119 EMAGE:31119 EMAGE:31119
euxassay_012730_01 euxassay_012730_02 euxassay_012730_03 euxassay_012730_04 euxassay_012730_05
EMAGE:31119 EMAGE:31119 EMAGE:31119 EMAGE:31119 EMAGE:31119
euxassay_012730_06 euxassay_012730_07 euxassay_012730_08 euxassay_012730_09 euxassay_012730_10
EMAGE:31119 EMAGE:31119 EMAGE:31119 EMAGE:31119 EMAGE:31119
euxassay_012730_11 euxassay_012730_12 euxassay_012730_13 euxassay_012730_14 euxassay_012730_15
EMAGE:31119 EMAGE:31119 EMAGE:31119 EMAGE:31119 EMAGE:31119
euxassay_012730_16 euxassay_012730_17 euxassay_012730_18 euxassay_012730_19 euxassay_012730_20
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euxassay_012730_21 euxassay_012730_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
choroid invagination
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 14 15 16 17 18
medulla oblongata part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
midbrain floor plate
moderate moderate
regionalmoderate expression: see section 11
metencephalon part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
medulla oblongata floor plate
moderate moderate
regionalmoderate expression: see section 11
metencephalon floor plate
moderate moderate
regionalmoderate expression: see section 11
diencephalon roof plate
moderate moderate
regionalmoderate expression: see section 11 12 13
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 10 12 13
spinal cord ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 11
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38096
Entity Detected:Ysk4, ( MGI:1203481)
Sequence:sense strand is shown

>T38096
GACCACAGATGTTACCGTGAGATACGTACGGCTCCATCAGGCCGATGTGTCGTTAATAAATGCCAGTCTT
CCGAAAGTGACAGGTGCAGCAACAGCAGGGCAAGACTTCTTCAGAAGAGACAACACATTAAACCCCCAAA
GCCATTGCATGGTCTCAGGCAAAAGCACAGAGGGTTCATTTCTAAAGACAAAGGTTGCAAGGATATGGGG
GGCCATACAGAGGATAGCGTTTCAGAACCTGATGGGCAGATGAAATCTCCAGGAAATGACTTTCTATCTT
CCAAAGATGACGCTCAACTCATGCACTTGATCCCCATCCCTGAACTGTCCCCCGAACAGAAAGCACCCGC
TCCAGTCTCAGATCTATCCATTGTTGAGGAAATCTTCACAGAGGAGTGTGCAGACGAAGAAGGCATTCTC
AACGATGACAGCCTTACCCAAAGCCTTGGCGATCTGAAAGAGCCTGAAGGGTTGCACCCCCAGGCCCCTC
TCGTTCCTTCAGAAAACAGCTGGGCTGTGCTTAGCGAGAAGCGTTCGGGCAAGCGTGTGTCTCCAGAAAA
GCACAATGTAGAACCTCTTGATAAAATAAATGCTGAGCAGATGTTCCCGGGTTACCTGGAGTTTGATAGC
CTTTCAGAGAAGTCTAAAACACTGGTGAGCTTTTCCAGTTGCTCTTTCCAGGAGAACCTGGAAAGAGCCC
CTTCCCCTACAGAACAACACTGGGCACGTTCTTTGGAGCAGGACAGCTTAGAAAATAACTCCACCACATA
CCAGACATTTGGAAAGATTTCCCAGGAAATCTTAGACCCAGGAAAGAATGAAGAGTTGACAGACGAGTTG
CTGGGTTGTCTAGTTGAAGAACTGTTAGCACTCGATGAGAAAGATAACAACTCTTGCCAAATAATGACAA
ATGAGGCAGACGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 79663. Forward Primer - name:079663_F_cDNA_Ysk4, sequence:GACCACAGATGTTACCGTGAGA; Reverse Primer - name:079663_N_SP6_cDNA_Ysk4, sequence:GCGTCTGCCTCATTTGTCAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012730 same experiment
 EMAGE:29286 same embryo
 EMAGE:29366 same embryo
 EMAGE:31147 same embryo
 EMAGE:31146 same embryo
 EMAGE:29299 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS