EMAGE mouse gene expression spatial database

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EMAGE:31186

C230094A16Rik ( MGI:2442895)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002686_01	euxassay_002686_02	euxassay_002686_03	euxassay_002686_04	euxassay_002686_05

euxassay_002686_06	euxassay_002686_07	euxassay_002686_08	euxassay_002686_09	euxassay_002686_10

euxassay_002686_11	euxassay_002686_12	euxassay_002686_13	euxassay_002686_14	euxassay_002686_15

euxassay_002686_16	euxassay_002686_17	euxassay_002686_18	euxassay_002686_19	euxassay_002686_20

euxassay_002686_21	euxassay_002686_22	euxassay_002686_23	euxassay_002686_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

weak

regional

weak expression: see section 01 02 03 22 23 24

ventral grey horn

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

olfactory cortex marginal layer

strong

regional

strong expression: see section 10 11 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 17

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 18 19

thoracic ganglion

moderate

regional

moderate expression: see section 11 12

not examined

regional

not examined expression: see section 01 02 03 22 23 24

midbrain

strong

regional

strong expression: see section 08 09 moderate expression: see section 06 07 10 11 12 13 14 15 16 17 18 19

diencephalon

strong

regional

strong expression: see section 08 09 moderate expression: see section 07 10 11 12 13 14 15 16 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 21 22 weak expression: see section 03 04 05 06 07 08 09 17 18 19 20

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 14 15 16 17

telencephalon marginal layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16

cervical ganglion

moderate

regional

moderate expression: see section 09 17

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 21 22 23 24 moderate expression: see section 18 19 20

hindbrain

strong

regional

strong expression: see section 08 09 moderate expression: see section 06 07 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5293
Entity Detected:	C230094A16Rik, ( MGI:2442895)
Sequence:	sense strand is shown >T5293 CGTCTGAGAAGCAAGAGAAGCAAGGACCTCCCTGGCTCTGGCTTTAGCTAGCTGCCGGGCCAGCAGCCTT TTATTCAACCCTGTGCTGCTTCTGACCTCTGTTTCAGACCACCCGTTAGCCGTGCAGCCCCCCAGCCCGA GAAACTCCAGAAGAACAACATCACCAAGAAAAAGAAGCTGGTTGAGGAGCTGGCTCTGGACCACGTGTTT GGCTACCGAGGATTTGACTGCCGCAACAACCTGCATTACCTTAACGATGGTGCCGACATCATTTTCCACA CTGCGGCAGCTGGCATTGTGCAGAACCTCTCCACAGGCAGCCAGAGCTTCTACCTGGAGCACACGGATGA CATCCTCTGCCTCACAGTGAATCAGCACCCAAAGTACAGAAATGTGGTGGCTACCAGCCAGATAGGGACA ACACCTTCAATTCATATCTGGGATGCAATGACCAAACACACCCTTTCCATGCTGCGGTGCTTCCACACCA AGGGGGTGAATTACATCAACTTCAGTGCAACCGGGAAGCTCCTGGTGTCTGTGGGCGTGGATCCAGAGCA CACCATCACTGTCTGGAGATGGCAAGAAGGTACCAAGGTTGCCAGCCGAGGGGGCCACCTGGAACGAATA TTTGTGGTGGAATTTCGCCCTGACTCAGACACACAATTTGTATCTGTTGGAGTCAAACATATGAAGTTCT GGACTCTGGCAGGAAGTGCTCTGCTGTACAAGAAAGGGGTCATCGGGTCTATGGAAGCTGCCAAGATGCA AACGATGCTGTCCGTGGCTTTTGGTGCTAACAACCTTACTTTTACTGGTGCCATCAATGGAGATGTCTAT GTCTGGAAGGAGCACTTCCTCATCCGGCTGGTGGCCAAGGCTCACACAGGTCCTGTCTTTACAATGTACA CCACCCTCCGAGATGGGCTTATTGTGACTGGCGGAAAAGAACGACCGACTAAGGAAGGAGGAGCTGTAAA GTTGTGGGACCAGGAGATGAAGCGCTGTCGGGCCTTCCAGCTGGAAACAGGGCAGCTGGTGGAATGTGTG CGCTCGGTGTGCCGTGGCAAGGGAAAAATCTTAGTAGGAACCAAAGATGGTGAAATAATTGAAGTTGGGG AAAAAAGCGCTGCTTCAAACATCCTGATTGATGGACACATGGAGGGGGAGATCTGGGGCCTAGCCACACA CCCCTCCAAGGACATGTTCATCTCAGCCAGCAATGATGGCACAGCAAGGATCTGGGACCTGGCTGACAAG AAACTGTTAAACAAGGTGAACCTGGGCCATGCGGCCAGGTGTGCAGCCTATAGCCCTGATGGGGAGATGG TGGCCATTGGCATGAAGAATGGAGAGTTTGTCATCTTGCTGGTGAACACCCTGAAAGTTTGGGGGAAAAA ACGAGACCGGAAATCTGCCATCCAAGATATCAGAATCAGCCCAGACAACAGATTCTTAGCTGTGGGGTCT TCTGAGCAGACAGTTGACTTCTATGACCTTACTCAGGGCACAAGTCTGAACCGGATCGGCTACTGCAAAG ACATCCCAAGCTTTGTCATTCAGATGGACTTTTCTGCAGACAGCAAATACATTCAGGTGTCAACAGGAGC CTATAAGCGCCAAGTACATGAAGTTCCCCTGGGGAAGCAAGTAACTGAAGCCATGGTTGTTGAGAAGATC ACCTGGGCTTCCTGGACAAGTGTTCTCGGAGATGAAGTCATTGGAATCTGGCCACGAAACGCAGACAAGG CTGATGTCAACTGTGCATGTGTGACCCATGCTGGCTTGAACATTGTCACAGGGGATGACTTCGGGCTGCT GAAGCTCTTTGATTTTCCATGCACAGAAAAATTCGCCAAGCACAAACGTTACTTTGGTCACTCTGCTCAT GTGACGAACATCCGCTTCTCTTCCGATGACAAGTATGTGGTCAGCACAGGAGGAGACGACTGCAGTGTAT TTGTGTGGCGATGTCTGTAAACACTGGAACCCCGTGCACTATGGCTGCTTCTCCCAAGCTACTTGTAGGC AGTGCAGGGGCACCTCCTCGACGCCAACTGCTGTGGGACACACAAGCTCTGAACAGAGATGTTGTAGGAC TCTCATAATCTGAACTTTGCAAGATGGTGATGCCAAGAATGAATACTAAGACTGTTTGCTTCTGTTCTGG AGACTAAAACCTATACTTATTGTAACTTCACTAACGGCAAAATTGGGATGATGTAGCCCACAGATAAAAT TTACATCCTCAATTACCCTAACCAATATGTAGCGTTTAATTTGCATTAAGACTTTTACAAAGGTGTTTTG CTATTCTGTTTCTATGTATTTTAAGAATGTTCACTGGACACAATGTAAGTTGGAAGCACTTAAGTACAGA AATACCCTGTTTGCCACATTTAGCTTTCAAAACTAAATGAGCCATGTATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5362808 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5362808 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002686 same experiment
	EMAGE:30280 same embryo
	EMAGE:31193 same embryo
	EMAGE:30266 same embryo
	EMAGE:31212 same embryo
	EMAGE:29636 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source