EMAGE mouse gene expression spatial database

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EMAGE:31198

Dpf3 D4, zinc and double PHD fingers, family 3 ( MGI:1917377)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003306_01	euxassay_003306_02	euxassay_003306_03	euxassay_003306_04	euxassay_003306_05

euxassay_003306_06	euxassay_003306_07	euxassay_003306_08	euxassay_003306_09	euxassay_003306_10

euxassay_003306_11	euxassay_003306_12	euxassay_003306_13	euxassay_003306_14	euxassay_003306_15

euxassay_003306_16	euxassay_003306_17	euxassay_003306_18	euxassay_003306_19	euxassay_003306_20

euxassay_003306_21	euxassay_003306_22	euxassay_003306_23	euxassay_003306_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

not examined

homogeneous

not examined expression: see section 01 02 03 04 23 24

neural retina

strong

regional

strong expression: see section 02 03 04 23 24 moderate expression: see section 01

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 15 16 18

telencephalon marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon mantle layer

strong

regional

strong expression: see section 04 07 08 10 11 12 13 14 15 16 19 20 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2112
Entity Detected:	Dpf3, D4, zinc and double PHD fingers, family 3 ( MGI:1917377)
Sequence:	sense strand is shown >T2112 TGGCCTCGAGCCAGATTCGGCACGAGGGCGACTGTCATTCACAACCCCCTGAAAGCGCTTGGGGACCAGT TCTACAAGGAAGCCATTGAGCACTGCCGGAGCTACAACTCGAGGCTGTGCGCAGAGCGGAGCGTGCGTCT CCCCTTCCTGGACTCGCAGACTGGGGTGGCTCAGAACAACTGCTACATCTGGATGGAGAAGAGGCACCGC GGCCCAGGCCTCGCTCCGGGCCAGTTGTACACATACCCTGCCCGCTGCTGGCGCAAGAAGCGACGATTGC ACCCACCAGAGGACCCAAAACTACGACTCCTGGAAATCAAACCCGAAGTAGAACTGCCCCTGAAGAAAGA TGGATTTACCTCTGAGAGTACCACACTGGAAGCCTTGCTTCGCGGCGAGGGAGTAGAGAAGAAGGTGGAT GCCAGAGAAGAGGAAAGCATCCAGGAGATACAGAGGGTTTTGGAAAATGATGAAAACGTAGAAGAAGGGA ATGAAGAGGAGGATTTGGAAGAAGATGTTCCCAAGCGCAAGAACAGGACCA
Notes:	The probe template was PCR amplified from IMAGE:851370 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:851370 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003306 same experiment
	EMAGE:31150 same embryo
	EMAGE:31152 same embryo
	EMAGE:29396 same embryo
	EMAGE:31466 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source