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EMAGE:31215

Capza1 (Capza1)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31215 EMAGE:31215 EMAGE:31215 EMAGE:31215 EMAGE:31215
euxassay_000273_01 euxassay_000273_02 euxassay_000273_03 euxassay_000273_04 euxassay_000273_05
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euxassay_000273_06 euxassay_000273_07 euxassay_000273_08 euxassay_000273_09 euxassay_000273_10
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euxassay_000273_11 euxassay_000273_12 euxassay_000273_13 euxassay_000273_14 euxassay_000273_15
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euxassay_000273_21 euxassay_000273_22 euxassay_000273_23 euxassay_000273_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
head mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
lung
moderate moderate
spottedmoderate expression: see section 05 06 07 09 10 11 12 13 16 17 18 19 20 21 22 23
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3626
Entity Detected:Capza1, (Capza1)
Sequence:sense strand is shown

>T3626
GTTTTTTTTGCTTTTTGCTCTCTGGCAGGATTCGTCTGCTTACAGAACAATCCTGCCAGAGAGCATAAAG
CAAAAATACCTATGATTCCTTCAATGTCTGTGTTTTCAAGTTTTATATTCTACTACATTGATAGTATGTC
AGCAGTTCTCAACTATGAAAGAGCAATACTAATGGGCAAAACTCTTCTAAAAATGCCAACATCTGTTGAG
GCGTTTCTGCAGGTATTGCCACTGGCAGGAAAAGTGGACCCAGGATAGATAAGAATGAAAACTTGGTATA
GCCAGACTTGGTCAGACTGGATCAAGACTTCTGGACTCTGATCCTATGTTTTAATTTTTCCACACATTTA
AACATAGTAGAACTCTGGGGAAAAGTCTCTGTGTGACAACTCTTATAACAAAGCTTCAGGGCCGGCTACA
TCTAAAGTCCCTTGCAAGGTAGTAAAGCACCTGTGACCTTTACAATAAACATGAACTCTATTGACTGAAA
AACAGAACTTAGGACGAGGGCCCAGA
Notes:The probe template was PCR amplified from IMAGE:3167992 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3167992 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000273 same experiment
 EMAGE:30087 same embryo
 EMAGE:31197 same embryo
 EMAGE:31195 same embryo
 EMAGE:29401 same embryo
 EMAGE:29302 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS