EMAGE mouse gene expression spatial database

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EMAGE:31216

Tspan6 ( MGI:1926264)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004870_01	euxassay_004870_02	euxassay_004870_03	euxassay_004870_04	euxassay_004870_05

euxassay_004870_06	euxassay_004870_07	euxassay_004870_08	euxassay_004870_09	euxassay_004870_10

euxassay_004870_11	euxassay_004870_12	euxassay_004870_13	euxassay_004870_14	euxassay_004870_15

euxassay_004870_16	euxassay_004870_17	euxassay_004870_18	euxassay_004870_19	euxassay_004870_20

euxassay_004870_21	euxassay_004870_22	euxassay_004870_23	euxassay_004870_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw molar

moderate

regional

moderate expression: see section 09 19 20

upper jaw molar

moderate

regional

moderate expression: see section 09 18 19 20

maxilla

moderate

regional

moderate expression: see section 09 10 11 19 20 21 weak expression: see section 07 08

mandible

moderate

regional

moderate expression: see section 09 10 11 18 19 20 21 22 23 weak expression: see section 04 05 06 07 08

lower jaw incisor

moderate

regional

moderate expression: see section 14 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 13 14 15 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6528
Entity Detected:	Tspan6, ( MGI:1926264)
Sequence:	sense strand is shown >T6528 CGGAAACTCTCGCGCCTGGCAAGCTCGAGCCGATCGCCCGGTGCATAGGCGCAGGGTCTCCGGGTAGTCA TGGCGTCCCCATCTCGGAGGCTGCAGACTAAGCCGGTCATTACTTGTTTGAAAAGCGTTCTCTTGATCTA CACTTTCATCTTTTGGATCACTGGTGTTATCCTTCTTGCCGTTGGCATTTGGGGCAAGGTGAGCCTGGAA AATTATTTTTCCCTCTTAAATGAGAAGGCCACCAATGTGCCTTTTGTGCTCATTGGCACAGGCACTGTCA TTATTCTCTTGGGCACCTTTGGTTGTTTCGCTACCTGTCGAGCCTCTGCCTGGATGCTGAAACTGTACGC GATGTTTCTGACACTCATTTTTTTGGTCGAACTAGTTGCCGCCATTGTTGGATTTGTTTTCAGACATGAG ATTAAGAACAGCTTTAAAAGTAACTATGAAAACGCTCTGAAGGAGTACAACTCCACAGGAGACTATAGAA GTGAAGCTGTAGACAAGATCCAAAGTACGTTGCATTGTTGCGGTGTCACCAATTACGGAGATTGGAAAGG TACGAACTATTACTCAGAAACAGGATTTCCCAAGAGCTGCTGTAAACTGGAGGGCTGTTATCCACAGAGA GATGCAGATAAAGTCAACGAAGAGGGTTGTTTCATAAAGGTAATGACAACTATAGAGTCAGAAATGGGAG TCGTTGCTGGAATTTCTTTTGGAGTTGCTTGCTTTCAGCTAATTGGAATCTTTCTTGCCTACTGCCTGTC TCGTGCCATAACGAATAACCAGTATGAGATAGTATAATCAGTACACACGGGCTCATATCTCCTCAGTTTT AAGGTTACCTTTGTGAGTTATAACAGCGCCTGGATGGAGACTGACATCTGCTTAGCCTGAAAAGCTACAC CAGTAGGCTGATTCAATCAAGATACTTGTGTATTGTATTCTGTGTCCACTTTCTGTCCTATTCTTACACC CCTGTATCTCTGGAACACTGGAAGTGCTAGTAAATTGTAAATGAAATAATACCCTGTTTCCCTTGGATTT GATTTCTCAGTGTAGGAATTGTGAGAAGTGCTGTGAACTTGCCATTTCCCTAACAGTGTCACTGGTGGAA CTTTAACCCCTATGAATTTGTCGTTGATGTAGTGTGATAGAAAATTGGTCTCCTTGAGTGGGCCTTGTAC TAGTCAAGATTGTCTGGCTTGATTATATAATCTGCTCCAAAAAAAGGAAAACATTTCCTTAGCCTCTGCT GGTAGGCAGAGTTGCATTGTATAATTTCATCCCAGCTGGTCATCAGATGGTGCCCATCAATGGCGTTCCT GCGTGGTGGTACCAGTCTCAGGCTTTGGGTGCAAAGGACAGAGACTAAGAGCCACCACTTGTATTCTGTG ATACATCAGCTTTCAGCACACAATGTATGTGCTTTTGTCACCAAACTAAGGCATTCATACGGATTCTACT TTATTATTTGAAAGGTTATAATTACTTTAATGGCATCAGAACTCTTGTATTTTCCTTATGAATTTACTGG AACTTACTTGATTAACTTTAGCTAGCTATTCCTCACCAAGCAAATCATACTTCATACCAACTGTCAGCAT TTTGGTTAATTTTAATAGTATTCATTTTGTTTTGTATCCTTGATTAATAAATATAAATCCTTTACACAAT AAATACTGCATTTCCTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3590862 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3590862 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004870 same experiment
	EMAGE:30229 same embryo
	EMAGE:31241 same embryo
	EMAGE:31213 same embryo
	EMAGE:30227 same embryo
	EMAGE:29347 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source