EMAGE mouse gene expression spatial database

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EMAGE:31225

BC037527 ( MGI:1917789)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005932_01	euxassay_005932_02	euxassay_005932_03	euxassay_005932_04	euxassay_005932_05

euxassay_005932_06	euxassay_005932_07	euxassay_005932_08	euxassay_005932_09	euxassay_005932_10

euxassay_005932_11	euxassay_005932_12	euxassay_005932_13	euxassay_005932_14	euxassay_005932_15

euxassay_005932_16	euxassay_005932_17	euxassay_005932_18	euxassay_005932_19	euxassay_005932_20

euxassay_005932_21	euxassay_005932_22	euxassay_005932_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23 moderate expression: see section 02 03 04 15

hand mesenchyme

moderate

regional

moderate expression: see section 01 02 03 23

upper leg mesenchyme

moderate

regional

moderate expression: see section 22 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

spinal cord

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13

foot mesenchyme

moderate

regional

moderate expression: see section 06 07 20 21

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

tail paraxial mesenchyme

strong

regional

strong expression: see section 10 11 12 13 14 15

tongue muscle

strong

regional

strong expression: see section 11 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8371
Entity Detected:	BC037527, ( MGI:1917789)
Sequence:	sense strand is shown >T8371 GGGACAGGAGCTGCCCTGAGTCCCGCCCCCCACCTGCCCCTTCCTCGGACCCGGCAAGAGATGGGTGCCC CCATCAGCACATTGTTCTTTGGCCACCTGACATCATGCCTCCCAAGAAGGATGCTCCCGTGAAGAAACCA GCAGGGCCCTCCATCTCTAAGCCTGCTGCCAAGTCTACACCAGGGACCCCTCTAGCCAAGGCCAAGGCTG AGCCGGCTGCCCCCCAGGCACCTGCAAAATCCCAGGAGCCCCCTGTTGATCTCTCCAAAGTGGTGATCGA GTTTAACAAGGACCAGCTGGAGGAATTCAGAGAGGCCTTTGAGCTGTTTGACCGAGTAGGTGATGGCAAG ATCCTGTACAGCCAGTGTGGGGACCTGATGAGGGCCCTGGGCCAGAACCCTACCAACGCCGAGGTGCTCA AGGTCCTGGGGAACCCCAAAAACGAGGAGCTGAAGTCCCGACGTGTAGACTTTGAGACGTTCCTGCCCAT GCTGCAGGCAGTGGCCAAGAACCGGGACCAAGGCACATATGAGGACTACCTAGAGGGGCTTCGTGTGTTT GACAAAGAGGGCAACGGCAAGGTCATGGGTGCAGAGCTCAGACATGTTCTTACCACTCTCGGAGAGAAGA TGACTGAGGAAGAGGTAGAGACTGTTCTGGCAGGCCACGAGGACAGCAACGGCTGCATCAACTATGAGGC CTTCCTGAAGCATATCCTAAGCCTCTGAGCTCCGCAGGTGGCCCTCCAGCCAGCCCCTGGTATCCGCTTG AGAGGCAAGGGTCCTCGATCCACCAGATAGAAGCGCAGGCAGTGCGTCTCCGCGCCCGTCCGTAGCGCAG CCGCCAGGGCTGGACTATTTCCTTCCCCACCCCCCACCCCCTCCCACCCCCACCCCACCCCAGCCCCGAC TCCCACCCCACGTAGAACCTGCAGCGACGGAAACTTGCCCCACCTCCTGTGCGGCCAGAAGCGCGTTCCT GCCACTAGGGGGCACTTACAGTTTTGAAATAAAAACATGGTTCCTGAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:3468485 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3468485 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005932 same experiment
	EMAGE:29428 same embryo
	EMAGE:29423 same embryo
	EMAGE:31226 same embryo
	EMAGE:29438 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source