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EMAGE:31254

Upk1a ( MGI:98911)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_012467_11 euxassay_012467_12 euxassay_012467_13 euxassay_012467_14 euxassay_012467_15
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euxassay_012467_21 euxassay_012467_22 euxassay_012467_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
heart ventricle
strong strong
spottedstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17
lung
strong strong
spottedstrong expression: see section 06 08 16 17 18 19 20 weak expression: see section 07 09
rectum
moderate moderate
regionalmoderate expression: see section 11
urethra of male
strong strong
regionalstrong expression: see section 11 12
bladder
strong strong
regionalstrong expression: see section 11 12 13
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38082
Entity Detected:Upk1a, ( MGI:98911)
Sequence:sense strand is shown

>T38082
GCTGATGCTCATCGTCTACATCTTCGAGTGTGCCTCCTGCATCACATCCTACACCCACCGCGACTATATG
GTGTCCAACCCATCCCTGATAACCAAGCAAATGTTGACCTACTACAGTGCAGACACTGACCAGGGCCAGG
AGCTAACCCGACTCTGGGACCGGATCATGATTGAGCAAGAGTGTTGTGGCACATCTGGCCCCATGGACTG
GGTGAATTACACATCAGCCTTCCGGGCAGCCACCCCGGAAGTGGTGTTCCCGTGGCCCCCACTGTGCTGT
CGTCGGACAGGCAACTTCATCCCCATCAATGAAGATGGCTGCCGAGTAGGCCACATGGACTACCTGTTTA
CCAAGGGTTGCTTCGAGCACATCGGCCATGCCATTGATAGCTACACGTGGGGCATATCGTGGTTTGGCTT
TGCCATCTTGATGTGGACCCTCCCTGTGATGTTGATAGCCATGTATTTCTACACCACTCTCTGAGGAGGA
AGGGGAGGCCATAGATGCACCTTGGCCTGAAGCTCCTGCCTCGCTGCTGCACATGCTTGGACAGCCTCAA
CCTCCTCCCCATCCCACCTCCGTGTACCTCAGCCTCAACCCACCCCATCCACCTCCCTCCATTTTCCCTT
CTTGTGATCCCAACATCCCTTTCTAGATGTAGGAGCTGCCTCGGGCCCTCCCTGTACCCCTGGGCCTGGA
TTTGTGTGCCCAGAACCTCTTGACCCTGCTTCACTTGGCTGACTACGGACAACAACATATCTTGTCCTTA
GAGGCCCTTCCGCTGCTCCTAACAACCCATCCTGCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 80328. Forward Primer - name:080328_F_cDNA_Upk1a, sequence:GCTGATGCTCATCGTCTACATC; Reverse Primer - name:080328_N_SP6_cDNA_Upk1a, sequence:GGCAGGATGGGTTGTTAGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012467 same experiment
 EMAGE:31670 same embryo
 EMAGE:31252 same embryo
 EMAGE:31251 same embryo
 EMAGE:29641 same embryo
 EMAGE:30532 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS