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EMAGE:31273

Adamdec1 ( MGI:1917650)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_001799_01 euxassay_001799_02 euxassay_001799_03 euxassay_001799_04 euxassay_001799_05
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euxassay_001799_06 euxassay_001799_07 euxassay_001799_08 euxassay_001799_09 euxassay_001799_10
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euxassay_001799_11 euxassay_001799_12 euxassay_001799_13 euxassay_001799_14 euxassay_001799_15
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euxassay_001799_16 euxassay_001799_17 euxassay_001799_18 euxassay_001799_19 euxassay_001799_20
EMAGE:31273
euxassay_001799_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hindgut
strong strong
regionalstrong expression: see section 11 12 13 14 15
foregut-midgut junction
strong strong
regionalstrong expression: see section 12 13 14 15 16
stomach
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08
midgut
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2684
Entity Detected:Adamdec1, ( MGI:1917650)
Sequence:sense strand is shown

>T2684
GGCCTCGAGGCCAGATTCGGCACGAGGCCTGACTGCAGTGAAGCATCCAACCACATCACAGAATGATCCA
GTGTCTAATTCACAGAGAAGCTACTTTGCCAGGACAGAAGTCCAGACCCTGCCTCTGCCAGGAAGCCCGC
CTATCCTGTGCACCTATACAGACAAACCTATCTCTGTGTGCCATGTTCTCTGTGAACCCATGACTTCAAT
CACTGAAGAAGAAGAATGCCCCAAAATACGACTTGTACCTGTCATGAATATGTAGGCATTTTCTCCTCAG
TACTCCATAAATAATAGAGTATAACAGCTATTTGTATAGCGTTTTTGTAGATTAAGTTTTAGAAGTAGTC
TAGAGATGACAAGATACTTAGAAGAATTTTAGTAGTTGTATGCAAACATACTCCAGGTCTAGAAAGACTG
TTAGTCCTGAGATTTGGGAATCTGTGTAATCATGGAGCCGAATCCTGGTGAACAATGAAGGAAGATCTTT
GCTTTTCTCTTTCCTTTCTACTTTATGTGTTTCTACCAGTCCAGTCAACAGGTCAATAGACATGCTAAGG
TGATGCTGTCTGTTCAT
Notes:The probe template was PCR amplified from IMAGE:1511966 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1511966 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001799 same experiment
 EMAGE:29989 same embryo
 EMAGE:31795 same embryo
 EMAGE:31877 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS