EMAGE mouse gene expression spatial database

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EMAGE:31331

Crat carnitine acetyltransferase ( MGI:109501)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003394_21	euxassay_003394_22	euxassay_003394_23	euxassay_003394_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 14 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 09 10 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 07 08 09 18 19 20 21 22 23 weak expression: see section 05 06

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6217
Entity Detected:	Crat, carnitine acetyltransferase ( MGI:109501)
Sequence:	sense strand is shown >T6217 CGCGGGGCCGCCTCTACTGGCTAGCGGCAGTGACTCGGCGACCTTGAACCTAGAACCGGAGCCGCGAACC CACTGACCCCAGCACCCCAGCAGTGGCGCAGTCTTTACCCAGCGCGAGGGGCGGAGCGGACCGAGTGTCC GCAAACAGACTGGGACGATGTTAGCCTTTGCTGCCAGAACCGTGGTAAAACCCCTGGGCCTCCTCAAGCC CTCCTCCTTGATGAAGGTTTCTGGTCGCTTCAAGGCCCACCAAGATGCACTGCCAAGGCTGCCTGTGCCC CCACTTCAGCAATCCCTGGACTATTACCTCAAGGCACTGCAGCCCATCGTGAGTGAGGAAGAATGGGCTC ACACCAAGCAACTGGTGGATGAGTTTCAGACCTCAGGGGGCGTAGGGGAACGCCTACAGAAGGGACTGGA GCGCAGGGCCAAGAAAATGGAGAACTGGCTGTCCGAGTGGTGGCTCAAGACAGCCTATCTCCAGTTCCGC CAGCCTGTGGTCATCTACTCCAGCCCAGGAGTGATCCTGCCCAAGCAGGACTTTGTGGATCTACAGGGTC AGCTTCGGTTTGCTGCCAAACTCATCGAGGGTGTATTGGATTTCAAGTCCATGATTGACAATGAGACGCT GCCAGTTGAGTTCCTGGGAGGACAGCCGCTGTGCATGAACCAGTATTATCAGATCCTGTCTTCTTGCCGT GTGCCCGGCCCCAAGCAGGACTCAGTGGTGAACTTCTTAAAGTCAAAGAGACCACCCACGCATATAACCG TGGTGCATAACTACCAGTTCTTCGAGCTGGATGTGTACCACAGTGACGGAACACCGCTCACCTCAGATCA GATCTTTGTGCAGCTAGAGAAAATATGGAATTCCTCACTGCAATCCAACAAAGAGCCTGTTGGCATCCTA ACCTCCAACCACCGAAACACCTGGGCCAAGGCCTACAACAACCTCATTAAAGACAAAGTTAACCGAGAGT CAGTGAACTCCATACAGAAGAGCATCTTCACCGTGTGCCTGGACAAGCAAGTGCCTAGAGTCTCAGATGA TGTCTACCGAAACCACGTAGCAGGCCAGATGCTACATGGTGGTGGTAGCAAGTTCAACAGTGGCAACCGC TGGTTTGACAAGACACTGCAGTTCATCGTGGCAGAAGACGGCTCCTGTGGGATGGTGTATGAGCATGCAG CTGCAGAAGGGCCCCCCATAGTTGCACTTGTGGACCATGTCATGGAGTATACAAAAAAGCCTGAGCTTGT GCGGTCTCCTATGGTGCCCTTGCCAATGCCCAAGAAGCTGCGGTTCAACATCACACCTGAGATCAAGAAC GACATAGAGAAAGCCAAACAGAACCTCAGCATCATGATCCAGGACCTGGACATCATGATGCTGACGTTCC ATCACTTTGGAAAGGACTTCCCCAAGTCCGAGAAGCTAAGCCCTGATGCCTTCATCCAGGTAGCCCTGCA GCTGGCATACTACAGGATCTATGGGCAGGCGTGTGCCACGTATGAAAGTGCCTCTCTGCGCATGTTTCAC CTGGGCCGCACTGACACCATCCGCTCAGCCTCCATAGACTCGCTGGCCTTTGTCAAAGGCATGGGTGACT CCACAGTGCCGGAGCAGCAGAAGGTGGAGCTGCTCCGGAAGGCTGTGCAGGCCCACCGAGCCTACACTGA CCGGGCCATCCGAGGGGAGGCCTTTGACCGGCACCTGCTGGGTCTGAAGCTGCAGGCCATCGAGGACCTG GTCAGCATGCCTGACATCTTCATGGACACCTCTTACGCCATTGCTATGCACTTCAACCTCTCTACCAGCC AGGTCCCTGCCAAGACAGACTGTGTCATGTTCTTCGGACCTGTGGTCCCAGATGGTTATGGCATCTGCTA CAACCCCATGGAGGCCCATATCAACTTCTCCGTGTCAGCCTACAACAGCTGTGCTGAGACCAACGCTGCC CGCATGGCTCACTACTTGGAGAAAGCTCTGCTGGACATGCGCACCCTACTCCAGAACCACCCCAGGGCCA AGCTCTGAGCCCAGGCCCAGACCTGCCACTTCCACAGCCAAACCCACTTTGGGATGGACACTTGCCATGG CTCACTTCCTTGGCTCCCGTGCCCTCCAGTTTGAGCCAGAAAAGCATTCTCTAGCAAGGCCCCAACGTCC ACACCAAGTGCCTTCCAGGCCCTGTCCTTAGCACCTGCCGGAGTCCATGTGCAGGGCTGAGTCCAGAGGC TGAACAAGACTACGGCCCAGGTTCCCAATGTATCATCCACTCCATGAACCATGGAGAAGAGAGCCAGTCC AGCCCATCCCTTCTCACAGCAGGAGCCGGGAAGAGTTCCTTTCCTCAGCCTGGCCAATCTCTTGTCATCT CCACAGCCAGGCCAAAATCTGTCTTCATTGGTAAATGCCCAAGGACCAGCGGTGGGAGGATTATCTATCA GTGAGGGCTCTCAACCTCCCCATAGCTAAGGGTTAAGTCTTTGTAGCCAAAGGGACATCTGAGGCCCAGC CTCCACCAGCCCACCTCAAGGTGCCATCTCCCTGCTGCCACACTCTGGTGACTCTTGAGCCCCAGACTTA TTGCTTCCATTCAGTGTGATTCTTGCTGCAGTCATGGTGGTATAATGTGATTATGCCAGGTGATCAATAA AGGGAAGAAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3499169 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499169 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003394 same experiment
	EMAGE:32130 same embryo
	EMAGE:32177 same embryo
	EMAGE:32129 same embryo
	EMAGE:31931 same embryo
	EMAGE:29768 same embryo
	EMAGE:32136 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source