EMAGE mouse gene expression spatial database

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EMAGE:31374

6330577E15Rik ( MGI:1915038)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002043_01	euxassay_002043_02	euxassay_002043_03	euxassay_002043_04	euxassay_002043_05

euxassay_002043_06	euxassay_002043_07	euxassay_002043_08	euxassay_002043_09	euxassay_002043_10

euxassay_002043_11	euxassay_002043_12	euxassay_002043_13	euxassay_002043_14	euxassay_002043_15

euxassay_002043_16	euxassay_002043_17	euxassay_002043_18	euxassay_002043_19	euxassay_002043_20

euxassay_002043_21	euxassay_002043_22	euxassay_002043_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 05 06 07 moderate expression: see section 04 08 09 10 11 12 14 15 16 17 18 19 20

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4618
Entity Detected:	6330577E15Rik, ( MGI:1915038)
Sequence:	sense strand is shown >T4618 CCCACGCGTCCGGCGCTCCGCTGGTTGTGTGTACGGTGTGTCGCCGCTGTGAGCATGGCTGAGGAAGGAA ATCAGGAGTTCACTTCCAAGATGGAAAACTCATCAGATTCAGCATCGACTTCACCTGATGCTCCTCAGCC CAGTGAGAATCCACCATCACCACCGACTTCACCTGCCGCTCCTCAGACCAGTGAGAATCCACCATCACCA CCGACTTCACCTGCCGCTCCTCAGCCCAGGGAGAATCCACCATCACCACCGACTTCACCTGCCGCTCCTC AGCCCAGGGAGAATCCACCATCACCACCGACTTCACCTGCCGCTCCTCAGCCCAGGGAGAATCCACCATC ACCCCATTCGAATAGTTCAGGAAAACAACCTCTGAGTGGAACACCTAAAGAAAGGTTAAAGAAAGCAAGA TCTTCATCTCATTCATTTTGTAGTGTGGTGAAGCGTATGAAGGTAGAGAATGACGAAAATAATGAGACCC TTTCAGAACCAGGAGAATCTTCAAAGGAAGAAAATTGTTCAAAGGCCCAAGAAAGCTTGAAAAACAAAGA CAGTGAGCCTGGAGAAAAGAGCTCAGAGGAGAAAAACACGTGTGAATCTAAGTCGTCGGATACTGGGTCA TCTAATGCCCTCCCAAAGGAGTCTGAGAATGCGATCATTAGAGAAAAATTAAAACAAGAAAAAATAAGAT TGATTAGGCAGGTTGAAGAGAAGGAAGACCTTCTTCGGAGGTTAAAGTTAGTCAAGATGTATAGAATAAA GAACGACGTGACTGAGTTAGAGAATCTAATAAAGAAGTGGAGGAAGTGTGGTCAGCGGCTGCTGTGTGAG CTGCAGTCTATCATGTCGGAGGACGAGGACGAGAAGCTGACGCTCACCGAGCTGATCGACTTCTACGGGA TAGATGACAACTTGCTGCACTACAATCGAAGTGAAGAAGAGTTTACAGGGGTGTGATACATTTTCCAGGC TATTTTCTCCCTCTCTTCTTTGGGTGATGTGATCATACCTAGGGCCTCAAAACATGTGCTCTGTTCCTGA GCTGTACACTTGGCCCATTTACAGATTGTCTTTGAGAATGGCAATGTAAAGAGAAATGTTTTAAAGGGAA AAAAAAAGATCTAAGCTGTTGTGGCTTAGAGGGAGGAATGTTGAACAGTTTGGGATATTCTGACAAAATT ATAAGACGAAGCTTAGTATTTTGGATATGTTTGCTAAAGAAAATATTTAAGTATAAAAAAATTAAAATTG TGTTTAAAAGGAAGTTTGGATAGTTCTAGAAACCAGGGTTAATTCATTGATTTGGGGGGCAAAAAGTTTA TATTCAGTATTAGAAAGTAGTATGTTGAATGTCTAAATCTCAAGTGGCATAAATGTAGCCTGCCATTCCT AAGTTTATGGACCTATGCTCATCTGCTTAACATATCTGTTTGCTAATCTAGCAACTAATTAAACTTAAAT GTCTACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5003059 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5003059 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002043 same experiment
	EMAGE:29490 same embryo
	EMAGE:31391 same embryo
	EMAGE:31364 same embryo
	EMAGE:31397 same embryo
	EMAGE:29503 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source