EMAGE mouse gene expression spatial database

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EMAGE:31401

AW549877 ( MGI:2146232)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002027_01	euxassay_002027_02	euxassay_002027_03	euxassay_002027_04	euxassay_002027_05

euxassay_002027_06	euxassay_002027_07	euxassay_002027_08	euxassay_002027_09	euxassay_002027_10

euxassay_002027_11	euxassay_002027_12	euxassay_002027_13	euxassay_002027_14	euxassay_002027_15

euxassay_002027_16	euxassay_002027_17	euxassay_002027_18	euxassay_002027_19	euxassay_002027_20

euxassay_002027_21	euxassay_002027_22	euxassay_002027_23	euxassay_002027_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 12 13 14 15 16

vagus x ganglion

moderate

regional

moderate expression: see section 08 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 weak expression: see section 21

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 15

spinal cord

weak

homogeneous

weak expression: see section 08 09 10 11 12 13

brain

weak

homogeneous

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17

thoracic ganglion

moderate

regional

moderate expression: see section 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T666
Entity Detected:	AW549877, ( MGI:2146232)
Sequence:	sense strand is shown >T666 CCTCGAGNCTGTTGGCCTACTGGCTCTTCGTGGTGTTTCTGATGCCTACAGCCTAAAGGTCTTGGGTTCT TTATTCACTTGAGGTGGTTTTCTGTCCACCGCCCAGTTCCCTTTTCTATCTTCTATACGGTTATTACATC TTTCCACAGAAACCAAAACTCTGTACTGTCTTTAGTATTACTTCCCTATTATTTTAAACACAGTATGAAG TAGCATTGTATGGAAACATTACAGTAAGAGGTACTTAATATAGACACAGCGTATGTATTATATCATGCTG GTGTTTGGTAGCTTTTGCTTTCCTGCGTCTAGTGAAAATTTATCTTCGGTTTGAATTCTCTGCTGTTGAT TAAATGTAAAATGTTACAGGATTTTCCCACAATCCTTAAACGTTTATCACTCTTTACTCAGTGTGAGTTT TCTGATGTGGAGTAGACTTTAAGACTTGATTTAAGAGCCTGTCTCTCTTGCTATGTTTATAGGCTGTCTG GCTGCTGTGCATTATTTCATGTTAAGTCACATATCATGACATCCCCTTATCCTTTGCTTAC
Notes:	The probe template was PCR amplified from IMAGE:1888204 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1888204 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002027 same experiment
	EMAGE:31571 same embryo
	EMAGE:30419 same embryo
	EMAGE:31566 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source