EMAGE mouse gene expression spatial database

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EMAGE:31482

Fbp1 ( MGI:95492)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000071_01	euxassay_000071_02	euxassay_000071_03	euxassay_000071_04	euxassay_000071_05

euxassay_000071_06	euxassay_000071_07	euxassay_000071_08	euxassay_000071_09	euxassay_000071_10

euxassay_000071_11	euxassay_000071_12	euxassay_000071_13	euxassay_000071_14	euxassay_000071_15

euxassay_000071_16	euxassay_000071_17	euxassay_000071_18	euxassay_000071_19	euxassay_000071_20

euxassay_000071_21	euxassay_000071_22	euxassay_000071_23	euxassay_000071_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

weak

homogeneous

weak expression: see section 07 08

thymus primordium

strong

spotted

strong expression: see section 08 09 10 11 12

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1478
Entity Detected:	Fbp1, ( MGI:95492)
Sequence:	sense strand is shown >T1478 TCGAGNCTGTTGGCCTACTGGCAGTCTTCCCGCTGTGACTATCACCTGTGGGCTCCACCTGCCTGCACCT TTAGTCAGCACCTTTAGCCAGCACCTGCGCCAGACCCCAGCAATGGCGAACCATGCGCCCTTCGAAACGG ATATCAGCACCCTGACCCGCTTCGTCATGGAGCAGGGCAGGAAGGCTCAGGGCACGGGGGAGTTGACCCA GCTGCTGAATTCGCTCTGCACCGCGATCAAAGCCATCTCGTCTGCGGTGCGCCAGGCGGGCATCGCACAG CTCTATGGTATCGCTGGCTCAACCAATGTGACTGGGGATCAAGTAAAGAAGCTGGACATACTTTCCAATG ACCTGGTGATCAATATGCTGAAGTCGTCCTACGCTACCTGTGTTCTTGTGTCTGAAGAAAACACAAATGC CATCATAATCGAACCTGAGAAGAGGGGCAAATATGTTGTCTGTTTCGATCCCCTTGATGGCTCATCCAAC ATTGACTGCCTTGTGTCCATCGGAACCATTTTTGGCATTTACAGAAAGAAAAGTACTGATGAGCCTTCTG AGAAGGATGCTCTG
Notes:	The probe template was PCR amplified from IMAGE:2537321 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2537321 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000071 same experiment
	EMAGE:31224 same embryo
	EMAGE:30794 same embryo
	EMAGE:30359 same embryo
	EMAGE:29358 same embryo
	EMAGE:31247 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source