EMAGE mouse gene expression spatial database

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EMAGE:31488

Carkl ( MGI:1921887)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001932_01	euxassay_001932_02	euxassay_001932_03	euxassay_001932_04	euxassay_001932_05

euxassay_001932_06	euxassay_001932_07	euxassay_001932_08	euxassay_001932_09	euxassay_001932_10

euxassay_001932_11	euxassay_001932_12	euxassay_001932_13	euxassay_001932_14	euxassay_001932_15

euxassay_001932_16	euxassay_001932_17	euxassay_001932_18	euxassay_001932_19	euxassay_001932_20

euxassay_001932_21	euxassay_001932_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 15 16 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T632
Entity Detected:	Carkl, ( MGI:1921887)
Sequence:	sense strand is shown >T632 TCAGNCTGTTGGCTACTTGCTTGGTCTGGAGGTTGAAGAATCCACTGTGTATTCACGCATGATCCAGGCG GCTGCACAGCAGAAAGACACCCATCTCACCATCACCCCAACAGTGCTGGGGGAGAGGCACCTGCCAGACC AGCTGGCCTCTGTGACTAGAATCTCCTCCTCTGACCTCTCCCTGGGCCATGTGACCCGGGCCCTCTGCAG AGGCATCGTTCAGAACCTGCACTCCATGCTTCCTTTCCAACAGCTCAAAGAGTGGGGTGTGGCCCGGGTG GTGGGGAGTGGGAGTGCACTGTCCAGGAACGAGGTGCTGAAGCAGGAGGTCCAGAGGGCGTTTCCTTTCC CAGTGTGCTTTGGGCAGGATGTAGATGCAGCCTTTGGGGCAGCTCTGGTCATGCTCCAGAGAGACCTTAG CCAGAAGGAGCCTTAGTTCATCAAGCTCCTTGGCTAAAGGAATGCTGGGAATCTTACCTAATTGACAATG TCAGCATGATCTCTGGGTTGTCCTTTTCTGGGACGGCTCTGT
Notes:	The probe template was PCR amplified from IMAGE:1886637 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1886637 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001932 same experiment
	EMAGE:31548 same embryo
	EMAGE:31492 same embryo
	EMAGE:30397 same embryo
	EMAGE:31511 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source