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EMAGE:31552

Pcdhb19 ( MGI:2136757)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_007699_01 euxassay_007699_02 euxassay_007699_03 euxassay_007699_04 euxassay_007699_05
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euxassay_007699_06 euxassay_007699_07 euxassay_007699_08 euxassay_007699_09 euxassay_007699_10
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euxassay_007699_11 euxassay_007699_12 euxassay_007699_13 euxassay_007699_14 euxassay_007699_15
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euxassay_007699_16 euxassay_007699_17 euxassay_007699_18 euxassay_007699_19 euxassay_007699_20
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euxassay_007699_21 euxassay_007699_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
facial vii ganglion
weak weak
regionalweak expression: see section 20
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 weak expression: see section 07 08 16 17 18 19 20 21
vestibulocochlear viii ganglion
weak weak
regionalweak expression: see section 07 16 17
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 07 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36751
Entity Detected:Pcdhb19, ( MGI:2136757)
Sequence:sense strand is shown

>T36751
ATGGTGAAGTATCGTACGCCTTTTCCCATGCATCAGAGGATATTCGAAAAACATTTGCAATTAATGAACA
TTCTGGAGAAATACGTTTAAAAGGCTATTTGGACTTTGAAACAACTGAATCCTACTCGATAATCATTAAA
GCTACTGATCGGGGAGGACTTTTTGGGAAATCTACAGTAAGAATTCAGGTGATGGATGTGAACGACAATT
TTCCTGAAATCATTGTGTCATCGTTTATCAGTCCAATCCCAGAAAATACCGCAGAGACTTTAGTTATGAT
TTTTAGCATTCGAGACAAGGACTCGGGTGAGAATGGCAAGATGGTGTGTACTATTCCGGAGGGTCTCCCG
TTTGTGCTAAAATCTTCTATTGAAAATTACTACCACTTAGAAACGGATGGAGCACTGGACAGAGAGAGCA
TAGCTGAATACAACATCACCATCTCTGTTACAGACCTGGGCACACCCAGGCTCACAACCCAGCACACCAT
AATAGTGCAGGTGGCCGACATCAATGACAATGCCCCTGCCTTCACCCAAACCTCCTATACCATGTTTTTC
CATGAGAACAACAGCCCTGCATTGCACATAGGCACCATCAGCGCCACAGACTCTGACGCTGGCTCCAATG
CCCACATCACCTACTCGCTGATGTCTGCTCAGGACCTTCAAATG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92064. Forward Primer - name:092064_F_cDNA_Pcdhb19, sequence:ATGGTGAAGTATCGTACGCCTT; Reverse Primer - name:092064_N_SP6_cDNA_Pcdhb19, sequence:CATTTGAAGGTCCTGAGCAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_007699 same experiment
 EMAGE:29553 same embryo
 EMAGE:29559 same embryo
 EMAGE:31479 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS