EMAGE mouse gene expression spatial database

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EMAGE:31582

Serpinb1a ( MGI:1913472)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009886_01	euxassay_009886_02	euxassay_009886_03	euxassay_009886_04	euxassay_009886_05

euxassay_009886_06	euxassay_009886_07	euxassay_009886_08	euxassay_009886_09	euxassay_009886_10

euxassay_009886_11	euxassay_009886_12	euxassay_009886_13	euxassay_009886_14	euxassay_009886_15

euxassay_009886_16	euxassay_009886_17	euxassay_009886_18	euxassay_009886_19	euxassay_009886_20

euxassay_009886_21	euxassay_009886_22	euxassay_009886_23	euxassay_009886_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 03 04 05 11 14 17 18 19 20 21 weak expression: see section 06 07 08 09 10 13 15 16

hindgut

moderate

regional

moderate expression: see section 14

liver lobe

strong

spotted

strong expression: see section 01 02 03 10 13 14 15 17 18 19 moderate expression: see section 04 05 06 07 08 09 11 12 20 21 22 23 weak expression: see section 24

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 05 12 14 17 18 19 weak expression: see section 06 07 08 09 10 13 15 16

mesenchyme

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

rectum

moderate

regional

moderate expression: see section 14

midgut

moderate

regional

moderate expression: see section 11 12 13 14 weak expression: see section 15

bladder

weak

regional

weak expression: see section 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30937
Entity Detected:	Serpinb1a, ( MGI:1913472)
Sequence:	sense strand is shown >T30937 TTCGCCTTGGAGCTGTTCCAAACCCTGAATGAAAGCAGCCCCACAGGAAACATCTTCTTCTCTCCCTTCA GCATTTCTTCTGCCTTGGCCATGGTCATTCTGGGGGCCAAAGGCAGCACTGCAGCTCAGCTTTCTAAGAC TTTTCATTTTGACTCTGTTGAGGACATCCATTCACGCTTCCAAAGCCTGAATGCTGAAGTGAGCAAACGT GGAGCATCTCACACTCTGAAACTTGCTAACAGACTGTATGGAGAGAAAACCTACAACTTCCTTCCTGAAT ACTTGGCTTCAACCCAGAAAATGTATGGTGCTGACTTGGCCCCTGTGGATTTTCTGCATGCCTCCGAGGA TGCAAGGAAGGAGATAAACCAGTGGGTCAAAGGTCAAACAGAAGGGAAAATCCCAGAACTGTTGTCTGTG GGTGTGGTGGACAGTATGACCAAACTTGTGCTGGTGAATGCCATCTACTTTAAGGGAATGTGGGAGGAGA AATTCATGACAGAGGACACAACGGATGCTCCATTCCGACTGAGTAAGAAAGACACAAAAACAGTGAAGAT GATGTATCAAAAGAAAAAATTTCCATTTGGTTACATTTCGGACCTGAAGTGCAAGGTGCTGGAGATGCCT TACCAGGGTGGAGAACTTAGCATGGTCATTCTGCTGCCTAAAGACATTGAGGACGAGTCCACGGGTCTTA AGAAGATTGAAAAGCAAATAACTTTGGAAAAACTGCTTGAATGGACCAAACGTGAGAACTTGGAATTCAT TGATGTCCACGTCAAACTGCCCCGGTTCAAGATAGAAGAGAGCTATACCCTCAACTCTAACCTGGGCCGC CTGGGAGTGCAGGATCTCTTTAGCAGTAGCAAGGCTGATCTCTCTGGCATGTCAGGATCCAGAGATCTTT TCATATCAAAAATTGTCCACAAGTCCTTTGTGGAAGTGAATGAGGAAGGAACAGAGGCAGCCGCTGCTAC AGGAGGCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4207111), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 35404. Forward Primer - name:035404_F_IRAV28-31_G19_Serpinb1a, sequence:TTCGCCTTGGAGCTGTTC; Reverse Primer - name:035404_R_SP6_IRAV28-31_G19_Serpinb1a, sequence:ATGCCTCCTGTAGCAGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009886 same experiment
	EMAGE:31339 same embryo
	EMAGE:31580 same embryo
	EMAGE:31562 same embryo
	EMAGE:30447 same embryo
	EMAGE:31557 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source