EMAGE mouse gene expression spatial database

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EMAGE:31640

Hoxc5 homeobox C5 ( MGI:96196)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008981_01	euxassay_008981_02	euxassay_008981_03	euxassay_008981_04	euxassay_008981_05

euxassay_008981_06	euxassay_008981_07	euxassay_008981_08	euxassay_008981_09	euxassay_008981_10

euxassay_008981_11	euxassay_008981_12	euxassay_008981_13	euxassay_008981_14	euxassay_008981_15

euxassay_008981_16	euxassay_008981_17	euxassay_008981_18	euxassay_008981_19	euxassay_008981_20

euxassay_008981_21	euxassay_008981_22	euxassay_008981_23	euxassay_008981_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

renal cortex

moderate

regional

moderate expression: see section 05 06 07 14 15 16 17

medulla oblongata

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15

mesenchyme

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 01 02 03 04 24

stomach

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 04 05

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35462
Entity Detected:	Hoxc5, homeobox C5 ( MGI:96196)
Sequence:	sense strand is shown >T35462 GAATCGGGTGCTTTCAACTAACCTTTGTAACTTCTAAACTGTCTTTGCTCATTAATTATTTGCATATGAA AGAGGGAGATAAGTGAAAGTGGGCCTGTAACCCCGGAGGTTGTCCTATTCTCTCTATACCTTCCAATCCC CTTCCCAAAGCCCGGGAACTTCCCTAGCCTGGCTTGCTGCATGCACTCTCAGGTTCACAGTTCCAGCCTG CTAGCTAGCTCAACTATTGGGGTCTCTTGTCTTTGGACCCTAGCAAGTTGTTCTTTGAGGCTTTTTTGCT ATCCTATATATCTTGCCATGACTTTCTCACCTTCCTGCCCCATTGCAGTTGCCCAACTATTTATTGACTC CAAGCCCTTCCTGAAACTATATTTTGTCATATCAAATAAAGCCAGAAAAAGAACAGGACTAAAGATTCAA TTACTTCTGTCTGTTTAGGGCATGCATTGAATTGATGTGTCTAAATAGGCTCTAAGATGTATTGGGAGGC CCAGTCTTTAAACCATCCCTTAAAAAATATTTTTAAACACCTTTAGGAGCAGCAAGAATAGGGAACAATG GAACTGAGTTCTTTTCTTGATTCATGGTTCCAAGAAGGGCCTCTGGGGTGAAGGGGAGTACACTTGAGGA CTCCTGTACTGTTGTTTCCACTTGCTGTGTGTGTCTAAGTTACCCAGACTTTTCTATATCCTATTCTCAA CGTTGGCTAAAACAACTTGAGACTTGAAGCTTCTAGACTGTAGTCTGTACTTTATGCCAGAGAATAGTAG CTCTGTGGCTCCACTGACTTAGATTCAGGCTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68517. Forward Primer - name:068517_F_cDNA_Hoxc5, sequence:GAATCGGGTGCTTTCAACTAAC; Reverse Primer - name:068517_N_SP6_cDNA_Hoxc5, sequence:GAGCCTGAATCTAAGTCAGTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008981 same experiment
	EMAGE:31625 same embryo
	EMAGE:30524 same embryo
	EMAGE:30512 same embryo
	EMAGE:31632 same embryo
	EMAGE:30521 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source