EMAGE mouse gene expression spatial database

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EMAGE:31830

Lbxcor1 (Lbxcor1)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000622_01	euxassay_000622_02	euxassay_000622_03	euxassay_000622_04	euxassay_000622_05

euxassay_000622_06	euxassay_000622_07	euxassay_000622_08	euxassay_000622_09	euxassay_000622_10

euxassay_000622_11	euxassay_000622_12	euxassay_000622_13	euxassay_000622_14	euxassay_000622_15

euxassay_000622_16	euxassay_000622_17	euxassay_000622_18	euxassay_000622_19	euxassay_000622_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 03 04 06 10 11 12 17 moderate expression: see section 08 09

facial vii ganglion

moderate

homogeneous

moderate expression: see section 05 13 18 19

medulla oblongata basal plate

moderate

homogeneous

moderate expression: see section 03 07 12 17 weak expression: see section 02

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 03 04 05 06 13 14 18 19 weak expression: see section 07 17

vestibulocochlear viii ganglion vestibular component

moderate

homogeneous

moderate expression: see section 04 05 06 weak expression: see section 17

superior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 04 06

vestibulocochlear viii ganglion cochlear component

moderate

homogeneous

moderate expression: see section 04 05 06

inferior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 04 not examined expression: see section 06

superior vagus x ganglion

moderate

homogeneous

moderate expression: see section 06 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1718
Entity Detected:	Lbxcor1, (Lbxcor1)
Sequence:	sense strand is shown >T1718 TGGCCTCGAGGCAAGATTCGGACGAGGGTGCAACAGCTGCAAATTGTCAGAGATACCTTGTGTAACGAAC TGGACCAGGAGAGGAAGGCCCGCTATGCCATCCAGCAGAAATTAAAAGAAGCTCACGACGCCCTGCACCA CTTCTCCTGCAAGATGCTGACACCCCGGCACTGCACCGGCAACTGCTCCTTCAAGCCTCCGCTGCTGCCC TAGCGCCGGCTTGGCCGCGCCCACGCGCCCTCAAGCCATGCTGCTCCTTTCTGTAAATACCCGCTGCAGT GGCGGCCCAGAGCGAGGAACAAGCCATTAGGACCTGACCGTTGTAAATACAGCCGCCCGCCCGCCGGTCC CCAGCCGGGCTCCGTTGGGTTTCCCATTGTAAATACTGCCTCGCCCCTCCTTTGAACTCCAGGGCATCAG ACCTCAAGGGGTAAACTGGACCCACCGGGGAAAGAAAGGGAAGAGGGGAGACCTCTTCTACGACCCCTCC CACTCGGGCCCGAGTAGGGCCTGGGACCCCGAATGTGAATATAACGTAGCATCTTCGCTGGCTAT
Notes:	The probe template was PCR amplified from IMAGE:479310 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:479310 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000622 same experiment
	EMAGE:30170 same embryo
	EMAGE:30111 same embryo
	EMAGE:30204 same embryo
	EMAGE:31864 same embryo
	EMAGE:30202 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	BrainStars same gene
	ViBrism same gene
Data Source