EMAGE mouse gene expression spatial database

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EMAGE:31833

Zfp830 zinc finger protein 830 ( MGI:1914233)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001953_01	euxassay_001953_02	euxassay_001953_03	euxassay_001953_04	euxassay_001953_05

euxassay_001953_06	euxassay_001953_07	euxassay_001953_08	euxassay_001953_09	euxassay_001953_10

euxassay_001953_11	euxassay_001953_12	euxassay_001953_13	euxassay_001953_14	euxassay_001953_15

euxassay_001953_16	euxassay_001953_17	euxassay_001953_18	euxassay_001953_19

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 12 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4565
Entity Detected:	Zfp830, zinc finger protein 830 ( MGI:1914233)
Sequence:	sense strand is shown >T4565 CCTGGGCGCAAACATGGCGTCCTCCACCTCTACTCGGACTCCGGCTGGGAAGCGAGTGGTGAATCAGGAA GAACTGAGGCGCTTGATGAGGGAGAAGCAGCGCCTGAGCACCAACCGGAAGAGGATCGAATCTCCATTCG CCAAGTACAACCGTCTGGGGCAGCTGAGCTGCGCACTGTGTAACACCCCGGTCAAGAGCGAGCTCCTGTG GCAGACCCATGTTCTGGGAAAGCAGCACCGGGAGAGAGTAGCGGAGCTGAAAGGCGCCAAAGGAGCAACC CAGGGCCCGTCGACCGGCACTGTACCTCAGGCCACCAAGAGGAGAGCGACGGATGTGGAGAGCCAAGACG CCAAGAAAGCCAAGGCCTCCGCGGGACCTCAGGTACAGCCCTCCACCTCAGCCTCGTCCGCCAACTTGGA TGCCGCTAGGGCGGCCCCCAGTAAACCTGGACTCGGTTTACTGCCTGATTATGACGATGAGGAAGAGGAG GAAGAAGAGGGAGGTGGAGAAGAAAGAAGGGACAGCAGCAAGCATCTTCCGGATGCACAGGGCAAAGAAC ACTCGCTTGCCTCTCCGAGGGAGACAACAAGCAATGTGCTGCCAAACGATCCGTTTAATACAAATCCTCC CAAGGCCCCCTTAGTTCCTCATTCAGGGTCCATTGAGAAAGCAGAAATACATGAAAAAGTGGTGGAAAGA AGAGAGAACACCGCCGAAGCATTACCAGAAGGGTTCTTCGACGACCCTGAGGTAGATGCAAAGGTCCGGA AGGTGGATGCCCCAAAGGATCAGATGGACAAAGAGTGGGACGAGTTTCAAAAGGCTATGAGGCAAGTCAA CACTATCTCTGAAGCGATCGTTGCTGAAGAGGATGAGGAGGGACGGTTGGACCGGCAGATTGGAGAGATT GATGAGCAGATAGAATGTTACCGCCGAGTGGAAAAGCTGCGGAATCGCCAGGATGAAATAAAAAACAAAC TTAAAGAGGTTTTGACCATAAAAGAACTTCAGAAAAAGGAAGAGGAGAATGTGGACAGTGACGACGAAGG AGAATTACAGGATTTGCTGTCTCAGGACTGGAGGGTAAAGGGGGCTTTGCTGTAAGGTTTTGAAGACCCA AGCTTTCCATCAGTCCCCGCTGTCCCTTAAAAAGTGCTGTGTCCAAGTTCTTGGGTTCCTCTGACCTTGG ATACCCCTAATTGTTGGGATAAAGTAAGCCAGTGAGGAGTAATACTTTGGGGATGGTACTGTGTGCGGTG CTATTTATTATAAAATGTTTGAGGTAAAACCGTTTTTGACATTTTTGAAATTATAACCACAAATTGCCAA CTTTTTCATCTTTACAGGTTCAATGTGGTTTTTCAGTTGTAAAATCTAAGTTGTAAATATTGCACATAGC TAAGTATATATTTATATAAGTCACTCTGTTTTGATCCTTATATTAGTCAGATAAAAAAGACACATGTTTA TATGACCTCGATGTATATATATTTTCAAGCCTTAAACTCCAAGGGTTGGCGCTTTGGGAAGGTTGTTTTT AGAAGGCCACTCATAAACACATATTCTTCATAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4981533 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4981533 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001953 same experiment
	EMAGE:31769 same embryo
	EMAGE:30558 same embryo
	EMAGE:30581 same embryo
	EMAGE:30608 same embryo
	EMAGE:30610 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source