EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31866

Slc36a2 solute carrier family 36 (proton/amino acid symporter), member 2 ( MGI:1891430)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003901_01	euxassay_003901_02	euxassay_003901_03	euxassay_003901_04	euxassay_003901_05

euxassay_003901_06	euxassay_003901_07	euxassay_003901_08	euxassay_003901_09	euxassay_003901_10

euxassay_003901_11	euxassay_003901_12	euxassay_003901_13	euxassay_003901_14	euxassay_003901_15

euxassay_003901_16	euxassay_003901_17	euxassay_003901_18	euxassay_003901_19	euxassay_003901_20

euxassay_003901_21	euxassay_003901_22	euxassay_003901_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

meckel's cartilage

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21

tibia

moderate

regional

moderate expression: see section 02 03

scapula

moderate

regional

moderate expression: see section 03 21

femur

moderate

regional

moderate expression: see section 04 05 21

liver

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

radius

moderate

regional

moderate expression: see section 01

palatal shelf

strong

regional

strong expression: see section 06 07 08 09 10 11 12 16 17 18 19 21

humerus

moderate

regional

moderate expression: see section 01 02 04 05 06 16 17 19 20 21 22

fibula

moderate

regional

moderate expression: see section 03 not examined expression: see section 02

rib

strong

regional

strong expression: see section 05 06 16 17 18 19 20 moderate expression: see section 03 04

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7320
Entity Detected:	Slc36a2, solute carrier family 36 (proton/amino acid symporter), member 2 ( MGI:1891430)
Sequence:	sense strand is shown >T7320 ATTCCAGCCAGGCCAGAGCAAGCTCTCCGGTCAGATCTACTGGGCCCAACTTGCACCTGCACATACACCT CCTTGATTGCCCTCTCCCTACCTTGAGCTTGCCCTTTCTGAAGCCATCATGTCTGTGACCAAGAGTGCCA GGAGTCCGCAGGTAGCCACCCCTCTCAATCTGGACCTTCCTGAGAGTGCCAAGAAGCTGCAGAGCCAGGA TCCCAGTCCAGCGAATGGGAGCTCTTCAGAGTCATCAAAGAAGACCAAGGGCATAACCGGGTTCCAGACA TTGGTTCATCTGGTCAAAGGCAACATGGGCACAGGGATCCTCGGACTTCCCCTGGCTGTGAAGAATGCGG GCATCTTGATGGGCCCACTCAGCTTGCTGGTAATGGGCCTCATCGCCTGCCACTGTATGCACATCCTAGT CAGATGTGCCCAGCGCTTCTGTCACAGACTGAACAAGCCTTTCATGGACTATGGGGACACAGTGATGCAC GGACTGGCTTTCAGTCCCAATGCCTGGCTGCAAAACCACGCCCACTGGGGGAGGCGTGTCGTGAGCTTCT TCCTCATCGTCACCCAGCTGGGTTTCTGCTGTGTGTACATTGTGTTTCTGGCGGACAATTTAAAACAGGT AGTAGAAGCTGTTAACAGCACAACCATCAGCTGCCACAAGAACGAGACGGTAGTTCTGACGCCCACCATG GACTCTCGACTCTACATGCTCTCCTTCCTGCCCGTTCTGGGGCTTCTGGTGTTCGTCAGGAACCTGCGTG TCCTCACCATCTTCTCCCTGCTGGCCAACATCAGCATGCTGGTCAGCCTGGTCATCATTGCCCAGTACAT CATCCAGGAAATTCCAGATGCCAGCCAGTTGCCACTGGTAGCAAGCTGGAAGACCTACCCGCTCTTCTTT GGAACAGCCATTTTTTCATTTGAAAGCATCGGTGTGGTCCTGCCTCTGGAAAACAAGATGAAGGATGCCC GCGGCTTCCCAACCATTCTGTCTTTGGGGATGTCCATCATCACCACCCTGTACATTGCTATCGGGGCTCT GGGCTACCTGCGGTTTGGAGATGACATCAAAGCCAGCATCACTCTTAACCTGCCCAACTGCTGGCTGTAC CAATCAGTCAAGCTCCTGTATGTCGTCGGTATCCTGTGTACCTATGCCCTGCAGTTCTACGTCCCTGCGG AAATCATCATCCCCCTTGCTGTCTCCCAGGTGTCAAAACGCTGGGCACTGCCCGTGGACCTGTCTATCCG GCTTGCCCTGGTCTGCCTCACATGCATGCTGGCCATCCTCATCCCACGCCTGGACCTGGTCCTCTCCCTG GTGGGCTCTGTGAGCAGCAGCGCCCTGGCCCTCATCATCCCGCCCCTGCTGGAGGTGGTCACCTACTATG GAGAGGGCATTAGCCCCCTGACCGTCACCAAGGATGCCCTCATCAGCATCCTGGGCTTCATGGGCTTTGT GGTGGGGACCTACCAGGCCCTGGATGAGCTGATCAAGTCCGGGAACTCTCCCGCCCTGTCCAACTCCACC ATGTTTATTCAGTGACAGAGCCTCTCCTCGCCCACGGTGACTTATGTTGTCTATGAATTTTTTCACTTTG CCTTTTCTCTGGTCCAAGACAGGAAAAAACAAAACAAAACAAAAAACAGGTGTAGCATATCCCCAGTACC GTGTGTGTGTGCGCGCGCGCAAAGGAGATGGGGGCCATCTACACCGCATACAAGGTGTAGATAGATGGTC ATTAAAAACAAAAGAAATGTTGGTCGTTCTCTAGTTTTCCACACTGTGGAGACCTCACCTGTGGAAACCG CCATCTTGTTTTTGTCATTTATCTGCTAATTTCCTGCCTTGAGTGGAGCTATGCAAAAAGCAACCACTAG GCGGCAGCAATAGCCAGCTTTTGGCAGAAGGTAGTCTCTGCCCGCTGAGGCTTTGGTTATTTCCTTTTCC TGACCATGATGGGGAACACTGTGCTCAAGGCAGCATCAAGATGGAGGGGGCCAGGGCCAGTCACAATGCT CAGATAAAATGTCACCATCTATGTTACAGATAAGTGTGTTGAACTTTCTAAACCACTAAGTCTTAGCCCA ACTCAGAAATCCAGCAGTGAACAAGAAAAACATAGGGTCATGCCTATGGGCCAGGGGCAGCAGACAGAGA CATCATTGGAGTCGTTTTTTTACTTCTGAATTCTTGCAATGAACATCTTCTGTTACCTGTCGGGCACCAA GCATAATACAGGGCTCTTATATAATAGTTTGGGTAAAGGTGTTATAAAAACAAGAGCTTATATAACATTG TAGCTTTGTGTACCCAACAGTAGAGTCTACGGGTCATCTCCTGAGTCTTGACCTTGTAACTTGCCATGGT CAACAGTAAAAGTGTTACAAATGGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4189221 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4189221 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003901 same experiment
	EMAGE:31945 same embryo
	EMAGE:29749 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source