EMAGE mouse gene expression spatial database

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EMAGE:31905

Slc28a3 solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 ( MGI:2137361)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6660
Entity Detected:	Slc28a3, solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 ( MGI:2137361)
Sequence:	sense strand is shown >T6660 GCAGGTGGCCCTCTGACATAGCAGAGGTGGTGCTTGCTCTCTCTCTGTCTCTGAGCAGAGGATGTCCAGG GCAGACCCGGGAAAGAATTCCGAGCCCAGTGAGAGCAAGATGAGCCTGGAGCTGAGGCCAACAGCCCCCA GCGACCTGGGCCGCAGCAATGAAGCCTTTCAGGATGAAGACCTTGAACGGCAGAACACTCCAGGAAACAG CACAGTCAGGAACAGAGTTGTACAAAGCGGGGAACAAGGACACGCCAAACAGGACGACAGGCAGATCACG ATTGAACAGGAGCCTCTAGGAAACAAAGAAGACCCAGAGGATGATAGCGAAGATGAGCATCAGAAAGGGT TCTTGGAGAGGAAGTATGATACAATTTGTGAGTTTTGTAGAAAGCACAGAGTTGTTCTCCGAAGCACCAT CTGGGCCGTTTTACTGACAGGTTTCCTGGCCCTGGTGATTGCAGCCTGTGCGATAAGCTTCCACAGAGCC CTTCCTCTTTTTGTGATCACCTTGGTGACCATCTTCTTTGTCATCTGGGATCACTTGATGGCCAAATATG AACAGCGAATAGATGATTTCCTGTCTCCTGGCAGAAGGCTTCTCGACAGGCATTGGTTCTGGCTGAAATG GGTGGTCTGGAGTTCATTGATCCTGGCGATTATTCTCTGGCTGTCTCTTGACACTGCCAAACTGGGCCAA CAAAACTTGGTATCCTTTGGCGGACTCATCATGTACCTTATCCTGTTATTTCTGTTTTCCAAACATCCCA CCAGAGTTTACTGGAGGCCTGTCTTTTGGGGAATTGGACTACAGTTTCTTCTTGGGCTCTTAATTCTAAG AACTAGGCCTGGATTTGTTGCTTTTGATTGGATGGGGAGACAAGTTCAGACTTTCCTGGGGTACACTGAT ACTGGAGCCAGGTTTGTCTTTGGGGAAAAGTATACAGACCACTTCTTTGCATTTAAGATCCTGCCCATCG TGGTTTTCTTCAGCACTGTGATGTCTATGCTTTACTATCTGGGGCTGATGCAGTGGATCATCAGAAAGGT CGGGTGGCTAATGCTTGTCACGATGGGGTCATCTCCCATTGAGTCTGTAGTTGCTGCTGGCAACATATTT ATTGGGCAAACAGAGTCTCCACTACTGGTCCAACCATATTTACCACACGTCACCAAGTCGGAACTCCATA CCATCATGACAGCTGGCTTTGCCACGATTGCTGGAAGTGTATTGGGCGCATATATCTCTTTTGGGGTGTC GTCCACGCATTTGCTAACTGCTTCGGTCATGTCTGCGCCTGCAGCCCTGGCTGTGGCCAAACTCTTTTGG CCTGAGACAGAGAAACCCAAAATAACCCTCAAGAGTGCCATGAAGATGGAAAATGGTGATTCAAGGAATC TCCTGGAGGCAGCCTCTCAGGGCGCATCCTCGTCCATCCCTCTGGTGGCAAACATTGCTGCGAATCTGAT CGCCTTCCTGGCCTTGCTCTCCTTTGTGAACTCTGCCCTGTCTTGGTTTGGAAGCATGTTCAACTACCCA GAGCTGAGTTTTGAGCTCATTTGTTCCTACATCTTCATGCCCTTCTCTTTCATGATGGGAGTCGACTGGC AAGACAGCTTTATGGTCGCCAAACTCATAGGTTACAAGACATTCTTCAATGAATTTGTGGCTTATGACCA CCTCTCAAAATTAATCAATTTGAGGAAAGCGGCTGGACCTAAATTTGTGAATGGCGTGCAGCAATATATG TCAATTCGCTCTGAGACCATCGCAACCTATGCCCTCTGTGGTTTTGCCAATTTTGGTTCCCTAGGAATCG TGATCGGTGGACTTACATCCATAGCACCATCCAGAAAACGAGACATCGCCTCTGGAGCCATGAGAGCCCT GATTGCTGGCACCATTGCCTGCTTCATGACAGCCTGCATCGCAGGCATCCTCTCTGATACCCCGGTGGAT ATCAACTGCCATCACGTTCTAGAGAACGGCAGAGTACTCAGCAACACAACCGAAGTGGTGTCTTGTTGCC AAAATCTGTTCAACAGCACTGTTGCCAGGGGACCAAATGACGTCGTCCCAGGAGGAAACTTCAGCCTCTA TGCTTTGAAGAGCTGCTGCAACCTGCTGAAACCACCAACGCTGAACTGCGATTGGATCCCTAATAAATTG TGATCTCAGCCATGTGTCCAGTGGGATTCTGCATGCAGATGCTGGAGTTCCTGCCCTGGAGGGGATAGAA AACTCCGTCATGGACTCAGACTTAATGGCAAAGGAGGAACAAGGTCAGGAGTGAGTCCTCCTGAGCTACA GTGTCTCCCTTCTCCTTTCTCTCCTTAGCCCACCATCTCTGCATGGAGAACTAGGATGTTGTCCCTAGCT GAGGTGTGATCCTAAGCCCCAACAATATTTTCTGACTTTATAGTTTCAAGGCATTTCATGAAAAAATATG TGTATATAAACAAATATATACACATATATATGTCTGTGTGTATATGTATATATATACACATGTAATAGTA TATATATGCATACCATATATATTTATATAAATATATATATGGTAGTTCCCAGCAACTGGGCCAGATAGAT AGATAGATAGATAGATAGATAGATAGATAGATAGATAGATGTGTGTGTGTGTGTGTGTGTTAGTTCCCAA TATGATTTAAACACATCTCAGGCAAGGTCATTCCACCCTCACTCAAGGTCGTCTGAAATACCTTCAACTT GAATCTCATGTGAAGGAGGGCTATTCTGCAATGGCCCATTCATTCGCTACCATCCAGAAGTTTCTCATAG ATCAAGAGTCCTTCAGAAAAGCACGCAGGCCATACGAAAGCCAATACTTGAGTCCATAAACATAGAGCCT TGTAAGCAGCGAGCTTCCATCCACCTATGCTGCAGAGACTTCCCGCTAACTTCAACATCTTACAAATCTT TTATGGATATGTTTGAAATGCTCGGCTTCAAGATTCTAAGTCTCATCAGCCTCAGAGGCTTGTAAGCAGT CGTCCTCCATCTTTTTAAAGTTAACATTGTATTTTTTGAATGAATATCTTTTATTCTTTGGCAATTTTAC ACATGTGTATAATGTACTTTGATCATACCTACCCTTAATTCCCACTGCCCCCACCCTCCTAGACACCCCT CAGCTTGTCTCCACCTCACCTTCTTGCCCTCTCCTCTATGTTTTGTAACCTTCTGAGTCTAGGGCTGCCT GCCACAATGTTGCCTGGCACTGTTGGCTTGATCTTGTGGGAGTGACCATTTCTTCTGTAACTTACTAAGT CTAGGGTTGCCTGCAACAATGTTGCCTGGCACTGTTGGCTTGATCTTGTGGGAGTGACCATTTCTACCTT GAGCTCATGAGTGCAAAGTCTGTCCTGTTCGGAAGACAGCATCTCACAGCTCTTCCCCAGCCCCTGGCTC TCACATTCTTTCCTCCCCATCTTGAGCTCTATTCCCTGTGCCTCAAGGAAGTAGCTATAAATGTTCCAGT TGGGGCTGAGCACTCTGTAGTTGTTTATTCTCACATTTATCAGCCATGAGTCTCTGCATTGCTAAACTGG GGTTTAATCGTGTCTGTTTTCAGTACCCTGGCTAAAAGAAGTGGTCCCTATATGGGCTGACCTTAGGGAA ACATGCAGAAACAGTGTAATAGCTCTTAACTTTGCTGCTAGGAGCCAGATTTGGTGCACACTCCTGTATT CCAGCTCTGGGAAGCTGAGACAGGAAGATTATGAGCTCAAAACCAGCACAGGGTACATAGCAAGAGTGTG TCTCCAAGCAGCAACAGCAAAATTTATACAAGGTAATAACACTCTTCATTTCCACTCTGTGCTGTGAGCC AGAGGAAGTTCTGTGACAAGGAGGTGTCTGAGGCCATAAATGAACTATCCCTGTCACAAGAGGAAAACAG TGGACACTTTGAAGAAGTCAATAGACTACCATGGTGCCTGATATCCAAGTCTCTGTGATTGTGTATATAG ACCTGTTGGTGTGCAATTTTGTTACATTCCTGATTAAGACTGTAAAACATTAGTTAGTGCTCAGTCAAAA AGCCACGTAAGCGCCAACTATGAAGGACACTCAATGCCCACTGAGGTGGGGATGGCTTTCACTGGCTCTC CTGTCAATCAGCAAGACACCAGCATCATGAGTGCTCCCTGGGGGTTATCAGCAGAACTCGCTTTAACGAA ATAAGTATCGACGGTGCAGAGAACACACTGAGGAAAACCTGAGCCAAAGACGACTCAAAAGGAAGAGTGT CAGCGGGGAAAGGGTTATGCAATACCTTAGATGATGTATTCTGTTTCCCCTTCTGACCATGCACGAGAGC AATTAAAAAATAATCTATGCATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3600786 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3600786 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006705 same experiment
	EMAGE:31355 same embryo
	EMAGE:31908 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source