EMAGE mouse gene expression spatial database

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EMAGE:31973

Phtf2 ( MGI:1916020)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004990_01	euxassay_004990_02	euxassay_004990_03	euxassay_004990_04	euxassay_004990_05

euxassay_004990_06	euxassay_004990_07	euxassay_004990_08	euxassay_004990_09	euxassay_004990_10

euxassay_004990_11	euxassay_004990_12	euxassay_004990_13	euxassay_004990_14	euxassay_004990_15

euxassay_004990_16	euxassay_004990_17	euxassay_004990_18	euxassay_004990_19	euxassay_004990_20

euxassay_004990_21	euxassay_004990_22	euxassay_004990_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8041
Entity Detected:	Phtf2, ( MGI:1916020)
Sequence:	sense strand is shown >T8041 AAGAGGTAATCCGGCGGCACTAGCCTTCGCGACTCTGCGCGTCTCCTAGCCGCTTCCCAGCGGCGCGGCG CCGGCGGCTACGCTTAAAATGACGAATGTGTGATCTCAATGGAATAAATGGCGTCCAGAGTCACAGATGC TATAGTCTGGTATCAGAAGAAGATTGGAGCCTATGATCAACAAATATGGGAAAAGTCTGTTGAACAAAGA GAAATCAAGGGCTTAAGGAATAAACCAAAGAAAACAGCACATGTGAAACCAGACCTCATCGATGTTGATC TTGTGAGAGGGTCTGCATTTGCTAAGGCAAAGCCTGAAAGTCCTTGGACTTCTCTGACAAGGAAAGGGAT TGTTCGAGTTGTCTTTTTCCCTTTCTTCTCCCGCTGGTGGTTACAAGTAACGTCCAGAGTCATCTTCTCC TGGCTTCTTGTTCTCTATCTTCTCCAAGTTGCTGCAATAGTATTATTCTGTTCCGCTCCTAGCCCACATA GCATACCTCTAACTGAAGTGATTGGGCCAATATGGCTGATGCTGCTTTTGGGAACCGTGCATTGCCAGAT TGTTTCAACAAGAACACCCAAGCCTCCACTAGGCACAGGCGGTAAACGAAGAAGGAAATTAAGAAAAGCA GCCCATTTGGAAGTCCACAGGGAAGGGGACGGTTCTAGCACCACAGATAACACACAGGAGGGAGCGGTGC AGAGCTACGGCGCAGGCGCCCCTTACAGTGTTGGCACTGTCTTCAGAGACCTTTGGCTTGCTGCTTTCTT CTTATCAGGGTCAAAGAAGGCAAAGAATTCAATTGATAAGTCAACCGAGACTGACAATGGCTATGTGTCC CTCGATGGGAAGAGGACTGTAAAAAGCAGTGAGGATGGAGCACAGTACCATGAGCTTCAGTGTGAAACTG TTGGACCAGAAGACGCAGCCTGGGCCACACGAACGCCAAGGAGTGTTCCTGCCAAAGATACCCAAAGAAA GATAACAAATGTCTCTGATGAAGTATCCAGTGAGGAGGGTCCCGAAACAGGATACCCATTGCGTGGTCAT GTGGACAGAACTTCTGAAAGCGGTCTTCGAAATAGAAAGCCACACCATTATAAAAAGCATTATGCAAATG AGGATGCCCCTAAATCGGGTACTAGTTGCAGCTCTCGCTGTTCAAGTTCCAGACAGGATTCTGAGAGCAC AAGGCCAGAATCTGAAACCGAAGGTGTATTATGGGAAGACTTGTTACACTGTGCGGAATGCCGCTCATCT TGTACCAGTGAGACAGATGTGGGAAACCCTCAGATAAATCCATGTGGGAAGAAAGAATATAGAGACGACC CTTTTCATCAGAGTCATTTGCCCTGGCTTCATAGTTCCCACCCAGGATTAGAAAAAATCAGTGCTATAGT GTGGGAAGGTAACGACTGTAAGAAAGCAGACATGTCTGTACTTGAAATCAGTGGGATGATAATGAACAGA GTGAACAACCATGTCCCAGGCATAGGATACCAGGTATTTGGAAATGCCATCTCTCTAATCCTGGGCTTGA CCCCATTTGTTTTCCGACTGTCACAAGCAACAGACTTGGAGCAGCTCACAGCCCATTCTGCGTCAGAACT TTATGTGATTGCGTTTGGCTCTAACGAAGATGTCATGGTTCTTTCTATGGTCCTGATAAGTTTTGTGGTT CGAGTCTCTCTTGTGTGGATCTTCTTTTTTTTGCTGTGTGTAGCAGAAAGAACATATAAGCAACGATTAC TCTTTGCAAAACTCTTTGGACACTTGACGTCTGCAAGGAGAGCTCGCAAATCTGAGGTTCCGCATTTCCG GTTGAAGAAAGTACAGAATATAAAAATGTGGCTGTCTCTGCGTTCCTATCTTAAGCGCCGAGGCCCTCAG CGATCTGTGGATGTCATTGTCTCATCGGCCTTCTTACTGACAATCTCAGTTGTGTTTATCTGCTGTGCAC AGCTGCTCCACGTGCACGAGATCTTCCTTGACTGTCACTGTAACTGGGAGCTGGTGATCTGGTGCATCTC CTTAACGCTGTTCCTCCTGAGGTTCGTTACTCTTGGATCAGAAACCAGCAAGAAATACAGCAATACCTCA ATCCTACTTACGGAACAGATAAACCTCTACTTGAAAATGGAGAAGAAACCTAACAAAAAGGAGGAGCTGA CCCTTGTGAATAATGTGTTAAAGCTGGCTACGAAACTGCTGAAGGAATTGGACAGTCCCTTTAGGTTGTA TGGACTTACAATGAATCCGCTGCTGTATAACATCACCCAGGTTGTCATCCTTTCGGCTGTTTCCGGTGTC ATCAGTGACTTGCTTGGATTTAATTTAAAGCTGTGGAAGATCAAATCGTGACAGTTGAGAGAAATGGAGG TGTAGCCGGGATGCCAGAGCACGAGCGCTGATGGAGCTGGCTGGAAGCAGTGGGTGGCTCTGCTCCAGGA GTCTACTGGGAAAGAAGTGTTTACATCAGAATGTATTGTGCAATTCTTATATTTATTTTACTTGTCCACT GGTTTTGTTTTGTTTTGTGTGTGTGTGTGTGTATGTTTGTTTTCTTTCTTTTTTTCCTTTTAAACATTTA GTTTGTTTTAAGCATTGAAGTTTTTCATTGTTGAAAGGGTGGCAGAGCGGATCCAGATACCTGAGATCCT GGTAATTGTTGACGTAATTGACAAGCAGATTGTGAAAACAGAAGCCATTGCTAAGCACTGTTTCTTCATT AATGCCGTGAACTTGCCTTACTTGAGGAATACGTCTTTAACTCTGAAATAGTGCGTTGTCTCAGCTAAAC ACATGTCCTCTGGTAAACCAGGGTCCGCTTAGGATCGCTCTTGAAATACTTCAGAACAGTACTGGGATCT AGACTGACTAAGCTGCAGTTGAAGCACCCTTCAGTATTAATAGATGGTGTTGTATAACAGGTCAACAAGT GCTCTTTGATAATAAAACTCTTAATGGAGCAATAATTGTAAATGGTTACCATACTGTAAAATATTTTGAT AAAAATTAATTAGTAATAAATGTATTTATTTAAAACACTGAGCTGTTTGCACAGCTCCAACTGTGCATGC TCAAAATGTGCACTTTTTAAAATGGTTTCTTTTGGTGTATATCTCTATGTGGATATGTTACAGTGTACTA GGGCATAATATGGTATCCTTTTGAGTGAGGTCTATCCTCATCTCACAAGTGAAGTGCCTATTGTTATTAC TAAATAAAGTGCGTTACGTTTACTCAAAAGAATTTCCCATGGTACACTATAGCGTATGCTGTTCCTACCA CACTTAAATAAAGCTTAAGAATAAAGTTGTCTGATTGCTAGTTATAAAATTTCCAAATTTTCCAGAAGGA CAGCTCCAGCCTACCAGTTCTAGCTTCCAGATTTAATCTGGTGCACCCCTCCCCTGGCTCCATTACATAA GGGCTATTTTTAGATGCTTTTAACGTGGAAAGCTAGCTGCTCTCCCCTTCAGTAACTTGTCCGTGTCCGG TGGGAGCTTATCATGTTTCCCTTTGGCAAATAGGCAGATATGATAGTCCCTACATCGGGCCTTGATTCTA AGTTGTTACCTTTGTACAATCAGTAGTGTTTTAAGAATTACATGAAACAATGTTTTTGTGATTGTCTTGT TTAAGCCAACCATTTGGTTTTTAAAACAAAGAAATTATTTGAAACAATCTAGGATTTTCTTGGTGCAAAG TCATATTGAATATATTCATATTTTTAGCATATTTTAAGGTCTTTAAAATAATTAATTGTAAATAATTTAT TTGATTGGTGCAGTTCTAATTCGCAAATCACTCTTAAAATCAGGATGTGTGGAGACCAGAGCCATGTGTT AAATGTTGCCCTTCCCATTCCTCTGAGCAACCTCAGCTAAGCCTCACTGGAGAGTGCTCTCTGTACAGGA AACGGCGGACAGCATTGTGTTCCGTCTGCCTGCGCTCTAATATGTCCAATAATGACCAAAGTGCATTCTG GGTATTTTTCGAGGAATGTATTACTGGAGCTTTAAGAACATACTTAGTTTCTCATGTGAAGAATTAGGCT TTGTCTGATATGTTCCTTCCTATATTGTCTAATTTGAGGTTGTTTTTAGGCACTATATTTTATAAACTTT TTCAAAATAAGGTACATACCTATACAGAACTTACCATTTTGCACAGAGTATATCAAATATATTTTGAGAA TAAAAAAGTACAGCATGAGTTCTGTTAGGAATAAAGTGAGATTGTTGTATCTTAAAAAACTTATTTCAGA TTGGAGGTATTTTTGAGAAAAGTAGCTGAGCATACTGACTTAGGAAAATGCTTGCTTTAGGTTGCAGCTG TGTTTAACGTAGAGTTGGGGTGGATTGTACAGTATCGGCCGTGCCGTTAACGTGGGCAGCAGTAGAGTTC TAGGAGGAAAATGTGCTAAGCAGCTTCATAGTTCACAGAGCCATTTCAGAGTAGTAAGTGACATCCCTGC CTTTAAGTATACCATGAATGTAAGAAGTAGAAGTTGTAACATATCACATCATAGAGAAAAAGCAACCCAG AGGTAGAATTATGGCAAATGCTGTGTGCCGTTTCTTTGCCGTGTGTTTTTAAAAGTTTGAACAGCAAGTT TAAAATAAAACATTATAAGACTGAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30050876 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30050876 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004990 same experiment
	EMAGE:30638 same embryo
	EMAGE:32011 same embryo
	EMAGE:31949 same embryo
	EMAGE:31058 same embryo
	EMAGE:31650 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source