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EMAGE:32018

Chrng cholinergic receptor, nicotinic, gamma polypeptide ( MGI:87895)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32018 EMAGE:32018 EMAGE:32018 EMAGE:32018 EMAGE:32018
euxassay_000346_21 euxassay_000346_01 euxassay_000346_02 euxassay_000346_03 euxassay_000346_04
EMAGE:32018 EMAGE:32018 EMAGE:32018 EMAGE:32018 EMAGE:32018
euxassay_000346_05 euxassay_000346_06 euxassay_000346_07 euxassay_000346_08 euxassay_000346_09
EMAGE:32018 EMAGE:32018 EMAGE:32018 EMAGE:32018 EMAGE:32018
euxassay_000346_10 euxassay_000346_11 euxassay_000346_12 euxassay_000346_13 euxassay_000346_14
EMAGE:32018 EMAGE:32018 EMAGE:32018 EMAGE:32018 EMAGE:32018
euxassay_000346_15 euxassay_000346_16 euxassay_000346_17 euxassay_000346_18 euxassay_000346_19
EMAGE:32018 EMAGE:32018 EMAGE:32018 EMAGE:32018
euxassay_000346_20 euxassay_000346_22 euxassay_000346_23 euxassay_000346_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
masseter
strong strong
regionalstrong expression: see section 19 20 moderate expression: see section 03 04 07
supraspinatus
strong strong
regionalstrong expression: see section 22 23 24
mylohyoid
strong strong
regionalstrong expression: see section 18 19 20 21 22 23 moderate expression: see section 03 04 07 08 10 11
sterno-mastoid
strong strong
homogeneousstrong expression: see section 21 moderate expression: see section 04 07 08 10 11
pectoralis minor
strong strong
homogeneousstrong expression: see section 18 22 23 24 moderate expression: see section 04 08 09 10 11
psoas minor
strong strong
regionalstrong expression: see section 12 19 20
subscapularis
strong strong
regionalstrong expression: see section 22 23 24
tongue intrinsic muscle
strong strong
regionalstrong expression: see section 11 12 13 14 16
erector spinae
strong strong
regionalstrong expression: see section 10 11 12 18 19 20
ilio-psoas
strong strong
regionalstrong expression: see section 13 14 15 16 21 moderate expression: see section 10
pectoralis major
strong strong
homogeneousstrong expression: see section 18 21 22 23 24 moderate expression: see section 04 08 09 10 11
extrinsic ocular muscle
strong strong
regionalstrong expression: see section 20 21 22 23 moderate expression: see section 03 04
intercostal muscle
strong strong
regionalstrong expression: see section 12 16 23 moderate expression: see section 09 10
psoas major
strong strong
regionalstrong expression: see section 12 13 14 15 18 20 21 22 moderate expression: see section 10
tongue
strong strong
regionalstrong expression: see section 11 13 14 moderate expression: see section 16
tongue extrinsic muscle
strong strong
regionalstrong expression: see section 11 12 13 14 16 moderate expression: see section 17
deltoid
strong strong
regionalstrong expression: see section 21
tongue muscle
strong strong
regionalstrong expression: see section 11
latissimus dorsi
strong strong
regionalstrong expression: see section 22 23
diaphragm
strong strong
homogeneousstrong expression: see section 20 21 22 23 moderate expression: see section 04 05 06 07 08 16
anterior abdominal wall muscle
moderate moderate
regionalmoderate expression: see section 04
quadratus lumborum
strong strong
regionalstrong expression: see section 13 23 moderate expression: see section 10
pectoral girdle and thoracic body wall muscle
strong strong
regionalstrong expression: see section 23
gluteus maximus
strong strong
regionalstrong expression: see section 22 23
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3863
Entity Detected:Chrng, cholinergic receptor, nicotinic, gamma polypeptide ( MGI:87895)
Sequence:sense strand is shown

>T3863
TGGCCTCGAGGCNAGATTCGGACGAGGCTGCAGAGGAGGCGGGCCACCAGAAGGTGGTGTTCTACCTGCT
TATCCAGCGCAAGCCCCTCTTCTACGTCATCAACATCATCGCCCCCTGTGTGCTCATCTCCTCAGTCGCC
ATCCTCATCTACTTCCTTCCTGCTAAGGCGGGCGGGCAGAAATGCACAGTGGCCACCAACGTGCTCCTGG
CCCAGACTGTCTTCCTTTTCCTTGTGGCTAAGAAGGTGCCTGAGACCTCCCAGGCAGTGCCACTCATCAG
CAAGTACCTGACCTTCCTCATGGTGGTGACCATCCTCATCGTCGTGAACTCTGTGGTCGTGCTCAATGTG
TCCTTGCGGTCCCCCCACACACACTCCATGGCCCGTGGGGTCCGCAAGGTGTTCCTGAGGCTCCTGCCCC
AGCTGTTACGGATGCATGTGCGCCCACTAGCTCCAGCTGCTGTCCAGGATGCCCGGTTCCGACTCCAGAA
TGGCTCTTCCTCAGGGTGGCCCATCATGGCTCGAGAGGAAGGGGACCTCTGTCTGCCTCGAAGCG
Notes:The probe template was PCR amplified from IMAGE:425717 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:425717 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000346 same experiment
 EMAGE:32044 same embryo
 EMAGE:29705 same embryo
 EMAGE:29707 same embryo
 EMAGE:30058 same embryo
 EMAGE:29667 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS