EMAGE mouse gene expression spatial database

EMAGE:32062

Necab2 N-terminal EF-hand calcium binding protein 2 ( MGI:2152211)
TS23 (14.5 dpc)
in situ hybridisation

Data Images

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 14 15 16 17 18

telencephalon mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 18 19 20 weak expression: see section 21

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 10 11 12 moderate expression: see section 05 06 07 09 13

pons mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36329
Entity Detected:	Necab2, N-terminal EF-hand calcium binding protein 2 ( MGI:2152211)
Sequence:	sense strand is shown >T36329 TACCATCGATTCAGACAACACCAACCACGTGGACACCAAGGAACTGTGTGACTACTTTGTGGAGCACATG GGAGACTATGAAGATGTCCTGGCCTCCCTGGAGACTTTGAACCACTCAGTCCTGAAGGCCATGGGCTACA CTAAGAAGGTGTATGAAGGTGGGAGCAATGTGGACCAGTTTGTGACACGCTTCCTTCTGAAGGAGACAGC CAATCAGATCCAGTCGCTGCTCAGCTCTGTGGAAAGTGCTGTAGAAGCCATCGAAGAACAGACCAGCCAG ATCCGACAGGACCACTGCAAACCCAGCCATGCCGTCAACGAGAGCCGCTACGGAGGCCCTACTCCTCCCT ACATCCCCAACCACAAGCTTGTGGCCCCGGAGCCGATGAAGAGCCTGCCTGTGGCCACAGGGGAGCCAAA GGAGGATGGGCTGGAGGGCCAGATAAGCCGGTTGGCAGAGCTGATTGGGAGACTGGAGAGCAAGACCCTC TCGTTTGACCTGCAACAGCGCCTCTCGGATGAAGAAGGCACCAACATGCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92164. Forward Primer - name:092164_F_cDNA_Efcbp2, sequence:TACCATCGATTCAGACAACACC; Reverse Primer - name:092164_N_SP6_cDNA_Efcbp2, sequence:GTGCATGTTGGTGCCTTCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008081 same experiment
	EMAGE:30680 same embryo
	EMAGE:30592 same embryo
	EMAGE:30687 same embryo
	EMAGE:32027 same embryo
	EMAGE:30653 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source