EMAGE mouse gene expression spatial database

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EMAGE:32075

Sp6 trans-acting transcription factor 6 ( MGI:1932575)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_000590_06	euxassay_000590_07	euxassay_000590_08	euxassay_000590_09	euxassay_000590_10

euxassay_000590_11	euxassay_000590_12	euxassay_000590_13	euxassay_000590_14	euxassay_000590_15

euxassay_000590_16	euxassay_000590_17	euxassay_000590_18	euxassay_000590_19	euxassay_000590_20

euxassay_000590_21	euxassay_000590_22	euxassay_000590_23	euxassay_000590_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw incisor

moderate

regional

moderate expression: see section 03 04 15 18

upper jaw incisor

moderate

regional

moderate expression: see section 03 04 15 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T11
Entity Detected:	Sp6, trans-acting transcription factor 6 ( MGI:1932575)
Sequence:	sense strand is shown >T11 CCAGACCGTGTGTCGCTGCCCCAACTGCCTGGAGGCGGAGCGACTCGGGGCTCCGTGCGGGCCCGATGGG GGCAAGAAGAAGCATTTGCACAACTGCCACATCCCAGGCTGCGGCAAGGCATACGCTAAGACGTCGCATC TGAAGGCGCACCTGCGGTGGCACAGCGGTGACCGACCCTTCGTGTGCAACTGGCTTTTCTGCGGCAAGCG CTTCACGCGCTCCGACGAGCTGCAGCGCCACCTTCAGACCCACACCGGGACCAAGAAGTTCCCCTGTGCG GTCTGCAGCCGAGTCTTCATGCGCAGCGACCACCTGGCCAAACACATGAAAACCCACGAAGGCGCCAAAG AGGAGGCGGCGGCGGCGGCCCAAGGCGAGGGCAAGGCCGGTGGCGTGGTGGAACCACCCGGGGGCAAAGG CAAACGTGAGGCCGAAGGCAGCTCGGCGTCCTCCAACTGAGCCCCATGGATGTCACATACCTGCCGTTCT TTATTTGGGGGGGCG
Notes:	The probe template was PCR amplified from IMAGE:793227 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:793227 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000590 same experiment
	EMAGE:30171 same embryo
	EMAGE:30188 same embryo
	EMAGE:30205 same embryo
	EMAGE:30677 same embryo
	EMAGE:30422 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source